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2.7.1.25: adenylyl-sulfate kinase

This is an abbreviated version!
For detailed information about adenylyl-sulfate kinase, go to the full flat file.

Word Map on EC 2.7.1.25

Reaction

ATP
+
adenylyl sulfate
=
ADP
 +
3'-phosphoadenylyl sulfate

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.25 adenylyl-sulfate kinase

Cloned

Cloned on EC 2.7.1.25 - adenylyl-sulfate kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APS kinase domain (amino acids 25-227) of the PAPSS1 coding region are expressed as a GST-fusion protein in Escherichia coli
expressed as His-tag fusion protein in Escherichia coli BL21(DE3)
expressed in COS-1 and HEK293 cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-DE3
expression in Escherichia coli
expression in Escherichia coli as fusion protein with glutathione-S-transferase or maltose binding protein
expression in Escherichia coli cysD-mutant
-
expression of full-length PAPS synthase and 1-268 N-terminal fragment in COS-1 cells and Escherichia coli
expression of wild-type and S107A mutant enzyme in Escherichia coli
expresssion in Escherichia coli
-
gene MXAN3487 or cysC, DNA and amino acid sequence determination and analysis, sequence comparisons, the gene is encoded in the mcuABC, an operon coding for part of the chaperone-usher (CU) system
in vitro transcription/translation
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
structural gene cysC
-
the APS-kinase domain (25-227 amino acid resiudes) of the PAPSS1 coding region are expressed as a GST-fusion protein in Escherichia coli BL-21
-