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2.7.1.25: adenylyl-sulfate kinase

This is an abbreviated version!
For detailed information about adenylyl-sulfate kinase, go to the full flat file.

Word Map on EC 2.7.1.25

Reaction

ATP
+
adenylyl sulfate
=
ADP
 +
3'-phosphoadenylyl sulfate

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.25 adenylyl-sulfate kinase

Temperature Stability

Temperature Stability on EC 2.7.1.25 - adenylyl-sulfate kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 43
-
10 min incubation at 30°C and 35.5°C do not affect the enzyme's activity, however, treatments of 38.8°C and 41.6°C decrease its activity to 80% and 60% of control, respectively. Temperatures higher than 43°C almost completely deactivate the enzyme. After incubation at 25°C for 17, 26, 41 and 68 h, the enzyme activity is 91%, 109%, 101% and 114% of the control, respectively
35
-
inactivation above, reversible by cooling to 0-30°C
36
-
equilibrium between active and inactive enzyme form
37
-
no loss of activity after 2 h, recombinant enzyme
40
-
reversible inactivation above by subunit dissociation, kinetics, MgATP2- or MgADP- stimulate reactivation
43
-
reversible inactivation, t1/2: 1 min
60
-
complete inactivation after 15 min
60 - 100
-
1 min, 85% reversible inactivation
70
-
no significant loss of activity after 15 min
80
-
irreversible inactivation, t1/2: 47 min, pH 8, 0.023 mg protein/ml
95
purified recombinant enzyme, 10 min, inactivation and denaturation