Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the N-terminal truncation variant (APSKDELTA96) is completely insensitive to substrate inhibition by adenosine 5Â’-phosphosulfate | Arabidopsis thaliana |
R93A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme. The mutation modestly reduces kcat by 2fold and increases the Km for adenosine 5‘-phosphosulfate by 3fold | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00043 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.00048 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.00172 | - |
adenosine 5'-phosphosulfate | mutant enzyme R93A, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.00263 | - |
adenosine 5'-phosphosulfate | N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and Superdex-200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + adenosine 5'-phosphosulfate | - |
Arabidopsis thaliana | ADP + 3'-phosphoadenosine 5'-phosphosulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosine 5'-phosphosulfate kinase | - |
Arabidopsis thaliana |
APSK | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.1 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
82.3 | - |
adenosine 5'-phosphosulfate | N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C | Arabidopsis thaliana | |
136 | - |
adenosine 5'-phosphosulfate | mutant enzyme R93A, at pH 7.5 and 17°C | Arabidopsis thaliana | |
272 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00251 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
0.0375 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
31300 | - |
adenosine 5'-phosphosulfate | N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C | Arabidopsis thaliana | |
32700 | - |
adenosine 5'-phosphosulfate | oxidized wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana | |
189000 | - |
adenosine 5'-phosphosulfate | mutant enzyme R93A, at pH 7.5 and 17°C | Arabidopsis thaliana | |
567000 | - |
adenosine 5'-phosphosulfate | reduced wild type enzyme, at pH 7.5 and 17°C | Arabidopsis thaliana |