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Literature summary for 2.7.1.25 extracted from

  • Ravilious, G.E.; Westfall, C.S.; Jez, J.M.
    Redox-linked gating of nucleotide binding by the N-terminal domain of adenosine 5’-phosphosulfate kinase (2013), J. Biol. Chem., 288, 6107-6115.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information the N-terminal truncation variant (APSKDELTA96) is completely insensitive to substrate inhibition by adenosine 5Â’-phosphosulfate Arabidopsis thaliana
R93A the mutant shows reduced catalytic efficiency compared to the wild type enzyme. The mutation modestly reduces kcat by 2fold and increases the Km for adenosine 5‘-phosphosulfate by 3fold Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-phosphosulfate
-
Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00043
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
0.00048
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
0.00172
-
adenosine 5'-phosphosulfate mutant enzyme R93A, at pH 7.5 and 17°C Arabidopsis thaliana
0.00263
-
adenosine 5'-phosphosulfate N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and Superdex-200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5'-phosphosulfate
-
Arabidopsis thaliana ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
?

Synonyms

Synonyms Comment Organism
adenosine 5'-phosphosulfate kinase
-
Arabidopsis thaliana
APSK
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.1
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
82.3
-
adenosine 5'-phosphosulfate N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C Arabidopsis thaliana
136
-
adenosine 5'-phosphosulfate mutant enzyme R93A, at pH 7.5 and 17°C Arabidopsis thaliana
272
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00251
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
0.0375
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
31300
-
adenosine 5'-phosphosulfate N-terminal truncation variant APSKDELTA96, at pH 7.5 and 17°C Arabidopsis thaliana
32700
-
adenosine 5'-phosphosulfate oxidized wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana
189000
-
adenosine 5'-phosphosulfate mutant enzyme R93A, at pH 7.5 and 17°C Arabidopsis thaliana
567000
-
adenosine 5'-phosphosulfate reduced wild type enzyme, at pH 7.5 and 17°C Arabidopsis thaliana