2.7.1.25: adenylyl-sulfate kinase This is an abbreviated version! For detailed information about adenylyl-sulfate kinase, go to the full flat file .
Word Map on EC 2.7.1.25
Reaction
ATP +
adenylyl sulfate =
ADP +
3'-phosphoadenylyl sulfate
Synonyms 3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1
ECTree
Metals Ions
Metals Ions on EC 2.7.1.25 - adenylyl-sulfate kinase
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Cd2+
-
0.42 and 1.1 mM, maximal activation of forward reaction in the presence of 0.42 and 1.1 mM ATP respectively
NaCl
increased activity at high salt conditions, 100 mM
Zn2+
-
309% of activation with Mg2+
Co2+
-
0.42 and 1.1 mM, maximal activation of forward reaction in the presence of 0.42 and 1.1 mM ATP respectively, inhibitory in excess
Co2+
-
164% of activation with Mg2+
Co2+
-
5 mM, 58% of activation with Mg2+ or Mn2+
EDTA
assay with 0.25 mM EDTA
EDTA
-
assay with 0.25 mM EDTA
K+
-
10 mM, 60% stimulation compared to Mg2+
K+
-
assay with 100 mM KCl
Mg2+
-
activation
Mg2+
-
0.42 and 1.1 mM, maximal activation of forward reaction in the presence of 0.42 and 1.1 mM ATP respectively, maximal activation of reverse reaction at concentrations that equals the ADP concentration at 0.03 and 0.07 mM
Mg2+
-
affinity for adenosine 5'-phosphosulfate decreases by 15fold in the absence of Mg2+
Mg2+
assay with 2.5 mM MgCl2
Mg2+
-
assay with 2.5 mM MgCl2
Mg2+
Penicillium duponti
-
activation
Mn2+
-
-
Mn2+
-
0.42 and 1.1 mM, maximal activation of forward reaction in the presence of 0.42 and 1.1 mM ATP respectively, inhibitory in excess
Mn2+
-
1 Mn2+ ion per subunit in the absence of substrate, 2 Mn2+ bind per subunit when APS or the ATP analog 5'-adenylylimidodiphosphate are present, afffinity for Mn2+ increases 23fold when the enzyme is phosphorylated
Mn2+
-
274% of activation with Mg2+
Mn2+
-
5 mM yield the same reaction velocity as 5 mM Mg2+, excess inhibits slightly
additional information
-
not activated by Cu2+ or Na+
additional information
-
no monovalent cations required, stereochemistry of divalent metal ion coordination
additional information
-
enzyme uses multiple metal ions to catalyze phosphoryl group transfer
additional information
-
a divalent cation is required for activity
additional information
-
a divalent cation is required for activity