2.7.1.25: adenylyl-sulfate kinase
This is an abbreviated version!
For detailed information about adenylyl-sulfate kinase, go to the full flat file.
Word Map on EC 2.7.1.25
Reaction
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Synonyms
3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1
ECTree
Purification
Purification on EC 2.7.1.25 - adenylyl-sulfate kinase
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alumina C-gamma gel, ammonium sulfate, hydroxyapatite, Cellex CM, unstable upon column chromatography
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ammonium sulfate, Affi-gel blue, Matrix-gel green, Agarose A
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ammonium sulfate, affinity chromatography, gel filtration, ion-exchange chromatography
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ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose
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ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94
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ammonium sulfate, phenyl-Sepharose, Q-Sepharose, Sephacryl S-300, hydroxylapatite, pentyl- agarose, aminohexyl agarose, ATP-agarose
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ammonium sulfate, Sephacryl S-200, partially purified
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ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose
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DEAE-Sepharose, Matrex gel blue A, phenyl Cellofine
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immobilized metal ion affinity chromatography (Ni2+)
Ni-NTA column chromatography and Superdex-200 gel filtration
Ni-NTA column chromatography, Ni-NTA/benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Ni2+-column, recombinant enzyme
nickel affinity column chromatography and Superdex-200 gel filtration
PAP-agarose column chromatography
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protein solution is loaded on a Glutathione-Sepharose Fast Flow column and further purified by gel-filtration chromatography using Superdex 200
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protein solutions are applied on Blue-Sepharose columns and further purified by ion-exchange chromatography
recombinant enzyme using His-tag
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant protein using His-tag
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Ni-NTA column chromatography and Superdex-200 gel filtration
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Ni-NTA column chromatography and Superdex-200 gel filtration
nickel affinity column chromatography and Superdex-200 gel filtration
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nickel affinity column chromatography and Superdex-200 gel filtration
partial
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partial
Penicillium duponti
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recombinant protein using His-tag
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recombinant protein using His-tag
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