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2.7.1.25: adenylyl-sulfate kinase

This is an abbreviated version!
For detailed information about adenylyl-sulfate kinase, go to the full flat file.

Word Map on EC 2.7.1.25

Reaction

ATP
+
adenylyl sulfate
=
ADP
+
3'-phosphoadenylyl sulfate

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.25 adenylyl-sulfate kinase

Purification

Purification on EC 2.7.1.25 - adenylyl-sulfate kinase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alumina C-gamma gel, ammonium sulfate, hydroxyapatite, Cellex CM, unstable upon column chromatography
-
ammonium sulfate, Affi-gel blue, Matrix-gel green, Agarose A
-
ammonium sulfate, affinity chromatography, gel filtration, ion-exchange chromatography
-
ammonium sulfate, Blue Sepharose, Sephacryl S-200, PBE 94, Red Sepharose, phenyl-sepharose
-
ammonium sulfate, Giogel P2, Blue Sepharose, Sephacryl S-300, PBE 94
-
ammonium sulfate, phenyl-Sepharose, Q-Sepharose, Sephacryl S-300, hydroxylapatite, pentyl- agarose, aminohexyl agarose, ATP-agarose
-
ammonium sulfate, Sephacryl S-200, partially purified
-
ammonium sulfate, Sephacryl S-300, chromatofocusing, 2'5'-ADP-agarose
-
DEAE-Sepharose, Matrex gel blue A, phenyl Cellofine
-
immobilized metal ion affinity chromatography (Ni2+)
Ni-NTA column chromatography and Superdex-200 gel filtration
Ni-NTA column chromatography, Ni-NTA/benzamidine-Sepharose column chromatography, and Superdex 200 gel filtration
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Ni2+-column, recombinant enzyme
nickel affinity column chromatography and Superdex-200 gel filtration
PAP-agarose column chromatography
-
partial
protein solution is loaded on a Glutathione-Sepharose Fast Flow column and further purified by gel-filtration chromatography using Superdex 200
-
protein solutions are applied on Blue-Sepharose columns and further purified by ion-exchange chromatography
recombinant enzyme using His-tag
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant protein using His-tag