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Literature summary for 2.7.1.25 extracted from

  • Venkatachalam, K.V.; Fuda, H.; Koonin, E.V.; Strott, C.A.
    Site-selected mutagenesis of a conserved nucleotide binding HXGH motif located in the ATP sulfurylase domain of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase (1999), J. Biol. Chem., 274, 2601-2604.
    View publication on PubMed
Search for more literature for 2.7.1.25

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
G427A slightly lower APS kinase activity than wild-type Homo sapiens
G427A/H428A no APS kinase activity Homo sapiens
H425A no APS kinase activity Homo sapiens
H428A no APS kinase activity Homo sapiens
N426K 2fold higher APS kinase activity than wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O43252
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-column, recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine 5-phosphosulfate i.e. adenylylsulfate or APS Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate i.e. 3'-phosphoadenylylsulfate or PAPS, via a phosphorylated enzyme intermediate ?