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2.7.1.25: adenylyl-sulfate kinase

This is an abbreviated version!
For detailed information about adenylyl-sulfate kinase, go to the full flat file.

Word Map on EC 2.7.1.25

Reaction

ATP
+
adenylyl sulfate
=
ADP
+
3'-phosphoadenylyl sulfate

Synonyms

3'-phosphoadenosine 5'-phosphosulfate synthetase1, 3'-phosphoadenosine-5'-phosphosulfate synthetase, 5'-phosphoadenosine sulfate kinase, adenosine 5'-phosphosulfate kinase, adenosine 5'-phosphosulphate kinase, adenosine 5-phosphosulfate kinase, adenosine 5’-phosphosulfate kinase, adenosine phosphosulfate kinase, adenosine phosphosulfokinase, adenosine-5'-phosphosulfate 3'-phosphotransferase, adenosine-5'-phosphosulfate kinase, adenosine-5'-phosphosulfate-3'-phosphokinase, adenosine-5'phosphosulfate kinase, adenosine-5’-phosphosulfate kinase, adenylyl-sulfate kinase, adenylylsulfate 3'-phosphotransferase, adenylylsulfate kinase, adenylylsulphate kinase, APK, APK1, APK2, APK3, APK4, APS kinase, APS-kinase, APSK, APSK1, ATP adenosine-5'-phosphosulfate 3'-phosphotransferase, ATP sulfurylase-APS kinase, CysC, EhAPSK, EHI_179080, kinase, adenylylsulfate (phosphorylating), MgATP:APS 3'-phosphotransferase, More, MXAN3487, MXAN_3487, MXAN_RS16905, PAPS 2, PAPS synthase, PAPS synthetase, PAPSS, PAPSS 1, PAPSS1

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.25 adenylyl-sulfate kinase

Crystallization

Crystallization on EC 2.7.1.25 - adenylyl-sulfate kinase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are grown by hanging drop vapor diffusion at 25°C in 40% (v/v) ethanol, 1.0% (w/v) PEG 6000, 50 mM sodium acetate and 5 mM MgATP
APSK isoform 1 in complex with beta,gamma-imidoadenosine-5'-triphosphate, Mg2+, and adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.5, 200 mM MgCl2, and 15-17.5% (w/v) PEG 2000
in complex with adenosine 5'-phosphosulfate, hanging drop vapor diffusion method, using 100 mM HEPES, pH 7.25, 200 mM MgCl2, and 15–17.5% (w/v) PEG 2000
crystal structures of the PAPSS1 APS-kinase domain in complex with APS and in complex with the products PAPS and ADP are solved, both structures of isolated domain, obtained in different crystal forms, reveal symmetrical dimers
-
crystals are grown at room temperature using hanging drops containing equal volumes of protein and reservoir solution
hanging drop vapor diffusion method
in complex with ligands, hanging drop vapor diffusion method, using 0.2 M NH4NO3, 20% (w/v) PEG3350
crystals are grown in 1.7 M NaH2PO4, 300 mM K2HPO4 and 100 mM Na-succinate, pH 4.0 by hanging drop vapor diffusion at room temperature, crystal structure of E-ADP-APS ternary complex at 1.43 A, crystal structure of E-ADP binary complex at 2.0 A
in complex with AMP-PNP and adenosine-5'-phosphosulfate, hanging drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate, pH 6.5, 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) PEG 8000
hanging-drop vapor diffusion method