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0.0045
(E)-5-(2-bromovinyl)-2'-deoxyuridine
-
37°C, fusion protein with glutathione S-transferase
0.0243 - 1.441
1-(beta-D-arabinofuranosyl)-cytosine
0.062 - 0.357
1-(beta-D-arabinofuranosyl)-thymine
0.028
1-beta-D-arabinofuranosylcytosine
-
-
1.093 - 1.124
2',3'-dideoxycytidine
0.0207
2'-deoxy-2',2'-difluorocytidine
-
0.0181 - 3.82
2'-deoxyadenosine
0.0009 - 2.162
2'-deoxycytidine
0.0095 - 20.35
2'-deoxyguanosine
0.0009 - 5.02
2'-deoxythymidine
0.0217 - 0.09
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
-
0.0111 - 0.0117
3'-azido-2',3'-didehydro-3'-deoxythymidine
0.0072 - 0.0083
3'-azido-2',3'-dideoxythymidine
0.0022 - 0.0049
5-(E)-(2-bromvinyl)-2'-deoxyuridine
0.00068 - 0.1017
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
-
0.292 - 1.124
Dideoxycytidine
additional information
additional information
-
0.0243
1-(beta-D-arabinofuranosyl)-cytosine
-
37°C, pH 7.5, wild-type enzyme
1.441
1-(beta-D-arabinofuranosyl)-cytosine
-
37°C, pH 7.5, mutant enzyme Y70W
0.062
1-(beta-D-arabinofuranosyl)-thymine
-
37°C, pH 7.5, wild-type enzyme
0.357
1-(beta-D-arabinofuranosyl)-thymine
-
37°C, pH 7.5, mutant enzyme Y70W
1.093
2',3'-dideoxycytidine
-
mutant enzyme N45D/N64D
1.124
2',3'-dideoxycytidine
-
-
0.0181
2'-deoxyadenosine
pH 8.0, recombinant enzyme
0.109
2'-deoxyadenosine
-
pH 8.0, 22°C
0.119
2'-deoxyadenosine
-
recombinant mutant N45D
0.15
2'-deoxyadenosine
-
recombinant mutant V84S
0.153
2'-deoxyadenosine
-
recombinant mutant V84A/M88R/A110D
0.225
2'-deoxyadenosine
-
recombinant wild-type enzyme
0.225
2'-deoxyadenosine
pH 7.8, 22°C, recombinant enzyme
0.239
2'-deoxyadenosine
-
recombinant mutant A110D
0.36
2'-deoxyadenosine
-
recombinant mutant M88R/A110D
0.373
2'-deoxyadenosine
pH 8, 37°C
0.373
2'-deoxyadenosine
-
pH 7.6, 37°C, recombinant wild-type enzyme
0.388
2'-deoxyadenosine
-
recombinant mutant V84S/M88R/A110D
0.501
2'-deoxyadenosine
-
0.625
2'-deoxyadenosine
-
pH 7.6, 37°C, recombinant mutant Q81N
0.7
2'-deoxyadenosine
-
recombinant mutant V84A
0.852
2'-deoxyadenosine
-
recombinant mutant V84M
2.088
2'-deoxyadenosine
-
recombinant mutant M88R
3.16
2'-deoxyadenosine
-
mutant enzyme N45D/N64D
3.82
2'-deoxyadenosine
-
recombinant mutant N64D
0.0009
2'-deoxycytidine
-
recombinant mutant N45D
0.001
2'-deoxycytidine
-
pH 8.0, 22°C
0.0023
2'-deoxycytidine
-
recombinant wild-type enzyme
0.0023
2'-deoxycytidine
pH 7.8, 22°C, recombinant enzyme
0.0023
2'-deoxycytidine
-
37°C, pH 7.5, wild-type enzyme
0.0025
2'-deoxycytidine
-
recombinant mutant V84A
0.0026
2'-deoxycytidine
pH 8, 37°C
0.0037
2'-deoxycytidine
-
37°C, pH 7.5, mutant enzyme E52D
0.0058
2'-deoxycytidine
-
37°C, pH 7.5, mutant enzyme E52H
0.0061
2'-deoxycytidine
-
0.0087
2'-deoxycytidine
wild type enzyme, at pH 7.6 and 37°C
0.0113
2'-deoxycytidine
mutant enzyme Q81E, at pH 7.6 and 37°C
0.0126
2'-deoxycytidine
pH 8.0, recombinant enzyme
0.0129
2'-deoxycytidine
-
recombinant mutant V84S
0.091
2'-deoxycytidine
-
recombinant mutant A110D
0.0964
2'-deoxycytidine
-
mutant enzyme N45D/N64D
0.113
2'-deoxycytidine
-
37°C, pH 7.5, mutant enzyme R105H
0.118
2'-deoxycytidine
-
recombinant mutant N64D
0.205
2'-deoxycytidine
-
37°C, pH 7.5, mutant enzyme Q81N
0.246
2'-deoxycytidine
-
37°C, pH 7.5, mutant enzyme Y70W
0.584
2'-deoxycytidine
-
recombinant mutant M88R/A110D
0.627
2'-deoxycytidine
-
recombinant mutant V84A/M88R/A110D
0.674
2'-deoxycytidine
-
recombinant mutant V84M
1.039
2'-deoxycytidine
-
recombinant mutant M88R
2.162
2'-deoxycytidine
-
recombinant mutant V84S/M88R/A110D
0.0095
2'-deoxyguanosine
pH 8.0, recombinant enzyme
0.04
2'-deoxyguanosine
-
recombinant mutant V84A/M88R/A110D
0.147
2'-deoxyguanosine
-
recombinant mutant V84A
0.159
2'-deoxyguanosine
-
recombinant mutant V84S/M88R/A110D
0.257
2'-deoxyguanosine
-
recombinant mutant A110D
0.375
2'-deoxyguanosine
-
recombinant mutant V84S
0.412
2'-deoxyguanosine
-
recombinant mutant N45D
0.429
2'-deoxyguanosine
-
0.4307
2'-deoxyguanosine
wild type enzyme, at pH 7.6 and 37°C
0.549
2'-deoxyguanosine
-
recombinant mutant M88R/A110D
0.645
2'-deoxyguanosine
-
recombinant mutant M88R
0.654
2'-deoxyguanosine
-
pH 8.0, 22°C
0.665
2'-deoxyguanosine
-
recombinant wild-type enzyme
0.665
2'-deoxyguanosine
pH 7.8, 22°C, recombinant enzyme
0.985
2'-deoxyguanosine
-
pH 7.6, 37°C, recombinant mutant Q81N
1.612
2'-deoxyguanosine
mutant enzyme Q81E, at pH 7.6 and 37°C
1.911
2'-deoxyguanosine
-
recombinant mutant V84M
2
2'-deoxyguanosine
pH 8, 37°C
2
2'-deoxyguanosine
-
pH 7.6, 37°C, recombinant wild-type enzyme
2.004
2'-deoxyguanosine
-
mutant enzyme N45D/N64D
20.35
2'-deoxyguanosine
-
recombinant mutant N64D
0.0009
2'-deoxythymidine
-
recombinant mutants N45D
0.0012
2'-deoxythymidine
-
37°C, pH 7.5, wild-type enzyme
0.0025
2'-deoxythymidine
-
pH 7.6, 37°C, recombinant wild-type enzyme
0.0025
2'-deoxythymidine
-
recombinant wild-type enzyme, pH 7.6, 37°C
0.0031
2'-deoxythymidine
-
pH 7.6, 37°C, recombinant mutant F114A
0.0037
2'-deoxythymidine
-
37°C, pH 7.5, mutant enzyme E52H
0.0038
2'-deoxythymidine
-
37°C, pH 7.5, mutant enzyme E52D
0.0047
2'-deoxythymidine
-
pH 7.6, 37°C, recombinant mutant Q81N
0.0058
2'-deoxythymidine
pH 8.0, recombinant enzyme
0.0089
2'-deoxythymidine
-
37°C, pH 7.5, mutant enzyme R105H
0.0153
2'-deoxythymidine
-
0.022
2'-deoxythymidine
-
pH 7.6, 37°C, recombinant mutant F114Y
0.0232
2'-deoxythymidine
-
recombinant mutant N64D
0.1
2'-deoxythymidine
-
above, pH 7.6, 37°C, recombinant mutants I29H and Q81D
0.138
2'-deoxythymidine
-
0.231
2'-deoxythymidine
-
37°C, pH 7.5, mutant enzyme Q81N
0.251
2'-deoxythymidine
-
37°C, pH 7.5, mutant enzyme Y70W
0.79
2'-deoxythymidine
-
recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C
0.93
2'-deoxythymidine
-
recombinant mutant M88R, pH 7.6, 37°C
3.3
2'-deoxythymidine
-
recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C
5.02
2'-deoxythymidine
-
recombinant mutant V84A/M88R, pH 7.6, 37°C
0.0217
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
mutant enzyme Q81E, at pH 7.6 and 37°C
-
0.09
2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
wild type enzyme, at pH 7.6 and 37°C
-
0.0111
3'-azido-2',3'-didehydro-3'-deoxythymidine
-
recombinant mutant N64D
0.0117
3'-azido-2',3'-didehydro-3'-deoxythymidine
-
recombinant mutant N45D
0.0072
3'-azido-2',3'-dideoxythymidine
-
mutant enzyme N45D/N64D
0.0083
3'-azido-2',3'-dideoxythymidine
-
-
0.0022
5-(E)-(2-bromvinyl)-2'-deoxyuridine
-
37°C, pH 7.5, wild-type enzyme
0.0049
5-(E)-(2-bromvinyl)-2'-deoxyuridine
-
37°C, pH 7.5, mutant enzyme Y70W
0.00068
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
mutant enzyme Q81E, at pH 7.6 and 37°C
-
0.1017
6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
wild type enzyme, at pH 7.6 and 37°C
-
0.0014
ATP
-
-
0.0014
ATP
-
pH 8.0, 22°C, reaction with 2'-deoxycytidine
0.0022
ATP
-
pH 8.0, 22°C, reaction with thymidine
0.292
Dideoxycytidine
-
recombinant mutant V84A
1.124
Dideoxycytidine
-
recombinant wild-type enzyme
0.92
ganciclovir
-
recombinant mutant M88R, pH 7.6, 37°C
1.2
ganciclovir
-
recombinant N-terminally truncated mutant M88R, pH 7.6, 37°C
1.75
ganciclovir
-
recombinant N-terminally truncated mutant V84A/M88R, pH 7.6, 37°C
2.68
ganciclovir
-
recombinant mutant V84A/M88R, pH 7.6, 37°C
5.06
ganciclovir
-
recombinant mutant V84A/M88R/A110D, pH 7.6, 37°C
0.0009
thymidine
-
pH 8.0, 22°C
0.001
thymidine
-
pH 8.0, 22°C
0.0012
thymidine
-
recombinant wild-type enzyme
0.0012
thymidine
pH 7.8, 22°C, recombinant enzyme
0.0016
thymidine
pH 8, 37°C
0.0113
thymidine
-
recombinant mutant V84A
0.0242
thymidine
-
mutant enzyme N45D/N64D
0.0247
thymidine
-
recombinant mutant V84S
0.064
thymidine
-
recombinant mutant A110D
0.237
thymidine
-
recombinant mutant M88R
0.829
thymidine
-
recombinant mutant V84M
1.512
thymidine
-
recombinant mutant V84A/M88R/A110D
2.425
thymidine
-
recombinant mutant M88R/A110D
6.56
thymidine
-
recombinant mutant V84S/M88R/A110D
additional information
additional information
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
Km-values of chimeric enzymes
-
additional information
additional information
-
positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics
-
additional information
additional information
straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex
-
2.7.1.145 deoxynucleoside kinase
results (
results (
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