2.7.1.145: deoxynucleoside kinase
This is an abbreviated version!
For detailed information about deoxynucleoside kinase, go to the full flat file.
Word Map on EC 2.7.1.145
-
2.7.1.145
-
thymidine
-
drosophila
-
deoxycytidine
-
suicide
-
deoxyguanosine
-
herpes
-
medicine
-
deoxyadenosine
-
e-5-2-bromovinyl-2'-deoxyuridine
-
kinase-deficient
-
dttp
-
deoxythymidine
-
dado
-
1-beta-d-arabinofuranosylthymine
-
synthesis
-
agriculture
- 2.7.1.145
- thymidine
- drosophila
- deoxycytidine
-
suicide
- deoxyguanosine
-
herpes
- medicine
- deoxyadenosine
-
e-5-2-bromovinyl-2'-deoxyuridine
-
kinase-deficient
- dttp
- deoxythymidine
-
dado
- 1-beta-d-arabinofuranosylthymine
- synthesis
- agriculture
Reaction
Synonyms
AGCK, AgdNK, BmdNK, D. melanogaster deoxynucleoside kinase, deoxyribonucleoside kinase, deoxyribonucleoside kinase
ECTree
2.7.1.145 deoxynucleoside kinaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.1.145 - deoxynucleoside kinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP
1-beta-D-arabinofuranosylcytosine + ATP
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
1-beta-D-arabinofuranosylthymine + ATP
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
2',3'-didehydro-3'-deoxythymidine + ATP
2',3'-didehydro-3'-deoxythymidine 5'-phosphate + ADP
-
-
-
-
?
2',3'-dideoxythymidine + ATP
2',3'-dideoxythymidine 5'-phosphate + ADP
4.44% of the activity with thymidine
-
-
?
2'-deoxy-2'-fluoroadenosine 3'-triphosphate + 2'-deoxyadenosine
2'-deoxy-2'-fluoroadenosine 3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
low activity
-
-
?
3'-fluoro-2',3'-dideoxythymidine + ATP
3'-fluoro-2',3'-dideoxythymidine 5'-phosphate + ADP
-
0.03% of the activity with thymidine and 2'-deoxycytidine
-
-
?
adenosine-2'-deoxy-2'-fluoro-3'-triphosphate + 2'-deoxycytidine
adenosine-2'-deoxy-2'-fluoro-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
low activity
-
-
?
adenosine-2'-deoxy-3'-triphosphate + 2'-deoxyadenosine
adenosine-2'-deoxy-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
low activity
-
-
?
adenosine-2'-deoxy-3'-triphosphate + 2'-deoxycytidine
adenosine-2'-deoxy-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
low activity
-
-
?
adenosine-3'-deoxy-2'-triphosphate + 2'-deoxyadenosine
adenosine-3'-deoxy-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
-
low activity
-
-
?
adenosine-3'-deoxy-2'-triphosphate + 2'-deoxycytidine
adenosine-3'-deoxy-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
low activity
-
-
?
adenosine-3'-deoxy-3'-fluoro-2'-triphosphate + 2'-deoxycytidine
adenosine-3'-deoxy-3'-fluoro-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
-
low activity
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
high activity
-
-
?
ATP + 1-(beta-D-arabinofuranosyl)-cytosine
ADP + 1-(beta-D-arabinofuranosyl)-cytosine 5'-phosphate
-
-
-
-
?
ATP + 1-(beta-D-arabinofuranosyl)-thymine
ADP + 1-(beta-D-arabinofuranosyl)-thymine 5'-phosphate
-
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
ATP + 1-beta-D-arabinofuranosylguanine
ADP + 1-beta-D-arabinofuranosylguanine 5'-phosphate
-
poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
ATP + 2',2'-difluorodeoxyguanosine
ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate
-
poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R
-
-
?
ATP + 2',3'-didehydro-3'-deoxythymidine
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
ATP + 2'-deoxy-2',2'-difluorocytidine
ADP + difluorodeoxycytidine 5'-phosphate
2'-deoxy-2',2'-difluorocytidine is gemcitabine, dNK is an activator of gemcitabine
-
-
?
ATP + 2'-deoxyinosine
ADP + 2'-deoxyinosine 5'-phosphate
-
-
-
?
ATP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one
ADP + 2-amino-8-(1'-beta-D-2'-deoxyribofuranosyl)-imidazo[1,2-a]-1,3,5-triazin-4(8H)-one 5'-phosphate
-
-
-
?
ATP + 2-chlorodeoxyadenosine
ADP + 2-chlorodeoxyadenosine 5'-phosphate
-
-
-
-
?
ATP + 2-hydroxy-2'-deoxyadenosine
ADP + 2-hydroxy-2'-deoxyadenosine 5'-monophosphate
preferred substrate for enzyme mutant 4
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
ATP + 5-(E)-(2-bromvinyl)-2'-deoxyuridine
ADP + 5-(E)-(2-bromvinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone
ADP + 6-amino-5-nitro-3-(1'-beta-D-2'-deoxyribofuranosyl)-2(1H)-pyridone 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
ATP + adenosine
ADP + adenosine 5'-phosphate
low activity
-
-
?
ATP + arabinosyl-2-fluoroadenine
ADP + arabinosyl-2-fluoroadenine 5'-monophosphate
-
-
-
?
ATP + arabinosyladenine
ADP + arabinosyladenine 5'-monophosphate
-
-
-
?
ATP + arabinosyluridine
ADP + arabinosyluridine 5'-monophosphate
-
-
-
?
ATP + dideoxycytidine
ADP + dideoxycytidine 5'-phosphate
-
wild-type enzyme: low activity
-
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
ATP + ganciclovir
ADP + ganciclovir phosphate
-
poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R
-
-
?
ATP + guanosine
ADP + guanosine 5'-phosphate
low activity
-
-
?
ATP + inosine
ADP + inosine 5'-phosphate
very low activity
-
-
?
bromovinyldeoxyuridine + ATP
bromovinyldeoxyuridine 5'-phosphate + ADP
-
specificity constant 930 per s and M
-
-
?
difluorodeoxycytidine + ATP
difluorodeoxycytidine 5'-phosphate + ADP
-
i.e. gemcitabine. Efficient in vivo phosphorylation, specificity constant 4800 per s and M
-
-
?
(E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP
-
-
-
-
?
(E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP
-
54% of the activity with thymidine
-
-
?
(E)-5-(2-bromovinyl)-2'-deoxyuridine + ATP
(E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate + ADP
54% of the activity with thymidine
-
-
?
1-beta-D-arabinofuranosylcytosine + ATP
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
10% of the activity with thymidine and 2'-deoxycytidine
-
-
?
1-beta-D-arabinofuranosylcytosine + ATP
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
58.2% of the activity with thymidine
-
-
?
1-beta-D-arabinofuranosylcytosine + ATP
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
58.2% of the activity with thymidine
-
-
?
1-beta-D-arabinofuranosylcytosine + ATP
1-beta-D-arabinofuranosylcytosine 5'-phosphate + ADP
-
50% of the activity with thymidine and 2'-deoxycytidine
-
-
?
1-beta-D-arabinofuranosylthymine + ATP
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
15% of the activity with thymidine and 2'-deoxycytidine
-
-
?
1-beta-D-arabinofuranosylthymine + ATP
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
-
53.9% of the activity with thymidine
-
-
?
1-beta-D-arabinofuranosylthymine + ATP
1-beta-D-arabinofuranosylthymine 5'-phosphate + ADP
53.9% of the activity with thymidine
-
-
?
2',3'-dideoxyadenosine + ATP
2',3'-dideoxyadenosine 5'-phosphate + ADP
-
0.9% of the activity with thymidine
-
-
?
2',3'-dideoxyadenosine + ATP
2',3'-dideoxyadenosine 5'-phosphate + ADP
0.9% of the activity with thymidine
-
-
?
2',3'-dideoxycytidine + ATP
2',3'-dideoxycytidine 5'-phosphate + ADP
-
-
-
-
?
2',3'-dideoxycytidine + ATP
2',3'-dideoxycytidine 5'-phosphate + ADP
11.1% of the activity with thymidine
-
-
?
2',3'-dideoxycytidine + ATP
2',3'-dideoxycytidine 5'-phosphate + ADP
-
0.02% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2'-deoxyadenosine + ATP
2'-deoxyadenosine 5'-phosphate + ADP
-
specificity constant 33000 per s and M
-
-
?
2'-deoxyadenosine + ATP
2'-deoxyadenosine 5'-phosphate + ADP
-
-
-
-
?
2'-deoxyadenosine + ATP
2'-deoxyadenosine 5'-phosphate + ADP
96% of the activity with thymidine
-
-
?
2'-deoxyadenosine + ATP
2'-deoxyadenosine 5'-phosphate + ADP
-
5% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2'-deoxyadenosine + ATP
2'-deoxyadenosine 5'-phosphate + ADP
-
15% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
specificity constant 18000 per s and M
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
134% of the activity with thymidine
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
-
?
2'-deoxycytidine + ATP
2'-deoxycytidine 5'-phosphate + ADP
-
-
-
?
2'-deoxyguanosine + ATP
2'-deoxyguanosine 5'-phosphate + ADP
-
specificity constant 10000 per s and M
-
-
?
2'-deoxyguanosine + ATP
2'-deoxyguanosine 5'-phosphate + ADP
-
-
-
-
?
2'-deoxyguanosine + ATP
2'-deoxyguanosine 5'-phosphate + ADP
-
1% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2'-deoxyguanosine + ATP
2'-deoxyguanosine 5'-phosphate + ADP
53% of the activity with thymidine
-
-
?
2'-deoxyguanosine + ATP
2'-deoxyguanosine 5'-phosphate + ADP
-
4% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2'-deoxyuridine + ATP
2'-deoxyuridine 5'-phosphate + ADP
-
specificity constant 410 per s and M
-
-
?
2'-deoxyuridine + ATP
2'-deoxyuridine 5'-phosphate + ADP
-
113% of the activity with thymidine
-
-
?
2'-deoxyuridine + ATP
2'-deoxyuridine 5'-phosphate + ADP
113% of the activity with thymidine
-
-
?
2'-deoxyuridine + ATP
2'-deoxyuridine 5'-phosphate + ADP
-
90% of the activity with thymidine and 2'-deoxycytidine
-
-
?
2-chloro-2'-deoxyadenosine + ATP
2-chloro-2'-deoxyadenosine 5'-phosphate + ADP
-
126% of the activity with thymidine
-
-
?
2-chloro-2'-deoxyadenosine + ATP
2-chloro-2'-deoxyadenosine 5'-phosphate + ADP
126% of the activity with thymidine
-
-
?
3'-azido-2',3'-dideoxythymidine + ATP
3'-azido-2',3'-dideoxythymidine 5'-phosphate + ADP
-
-
-
-
?
3'-azido-2',3'-dideoxythymidine + ATP
3'-azido-2',3'-dideoxythymidine 5'-phosphate + ADP
2% of the activity with thymidine
-
-
?
3'-azidothymidine + ATP
3'-azidothymidine 5'-phosphate + ADP
-
0.5% of the activity with thymidine and 2'-deoxycytidine
-
-
?
3'-azidothymidine + ATP
3'-azidothymidine 5'-phosphate + ADP
-
0.3% of the activity with thymidine and 2'-deoxycytidine
-
-
?
5-fluoro-2'-deoxyuridine + ATP
5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
92% of the activity with thymidine
-
-
?
5-fluoro-2'-deoxyuridine + ATP
5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
92% of the activity with thymidine
-
-
?
5-fluoro-2'-deoxyuridine + ATP
5-fluoro-2'-deoxyuridine 5'-phosphate + ADP
-
activity is 10% higher than with thymidine and 2'-deoxycytidine
-
-
?
adenosine + ATP
adenosine 5'-phosphate + ADP
-
0.82% of the activity with thymidine
-
-
?
adenosine + ATP
adenosine 5'-phosphate + ADP
0.82% of the activity with thymidine
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
low activity
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylcytosine
ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
low activity
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 2',2'-difluorodeoxycytidine
ADP + 2',2'-difluorodeoxycytidine 5'-phosphate
-
best substrate for the N-terminally truncated mutant M88R
-
-
?
ATP + 2',3'-didehydro-3'-deoxythymidine
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
low activity
-
-
?
ATP + 2',3'-didehydro-3'-deoxythymidine
ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-phosphate
low activity
-
-
?
ATP + 2',3'-dideoxythymidine
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
ADP + 2',3'-dideoxythymidine 5'-phosphate
low activity
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
48% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
2.7% of the activity with 2'-deoxycytidine
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
-
best substrate for the N-terminally truncated mutant V84A/M88R
-
-
?
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
1% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
high activity
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
99% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
best substrate
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
wild-type enzyme: second-best substrate
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
88% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
high activity
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
83% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
0.5% of the activity with 2'-deoxycytidine
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
poor substrate for the wild-type enzyme
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
-
poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R
-
-
?
ATP + 2'-deoxyguanosine
ADP + 2'-deoxyguanosine 5'-phosphate
0.2% of the activity with 2'-deoxythymidine
-
-
?
ATP + 2'-deoxynucleoside
ADP + 2'-deoxynucleoside 5'-phosphate
-
-
-
-
ir
ATP + 2'-deoxynucleoside
ADP + 2'-deoxynucleoside 5'-phosphate
-
dNK has a significant preference for pyrimidine deoxynucleosides over purine deoxynucleosides, but it does phosphorylate deoxyadenosine and deoxyguanosine at significant rates
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + 2'-deoxyribonucleoside
ADP + 2'-deoxyribonucleoside 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
best substrate
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
high activity
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
65% of the activity with 2'-deoxycytidine
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
best substrate
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
2'-deoxythymidine binding structure at the active site, overview
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
best substrate for the wild-type enzyme
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
substrate binding structure of mutant N64D
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
best substrate
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
best substrate
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
high activity
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
-
-
?
ATP + 2-chloro-2'-deoxyadenosine
ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
low activity
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
low activity
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine
ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
low activity
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
high activity
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
high activity
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
poor substrate for the wild-type enzyme but moderate activity with N-terminally truncated mutants M88R and V84A/M88R
-
-
?
ATP + 9-beta-D-arabinofuranosyladenine
ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
very low activity
-
-
?
ATP + a 2'-deoxyribonucleoside
ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
-
-
-
?
ATP + a 2'-deoxyribonucleoside
ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
-
-
?
ATP + a 2'-deoxyribonucleoside
ADP + a 2'-deoxyribonucleoside 5'-phosphate
-
-
-
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
-
-
-
?
ATP + E-5-(2-bromovinyl)-2'-deoxyuridine
ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate
-
-
-
?
ATP + stavudine
ADP + stavudine phosphate
anti-HIV nucleoside analogue
-
-
?
ATP + stavudine
ADP + stavudine phosphate
anti-HIV nucleoside analogue
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
-
wild-type enzyme: preferred substrate
-
-
?
ATP + uridine
ADP + uridine 5'-phosphate
very low activity
-
-
?
cytidine + ATP
cytidine 5'-phosphate + ADP
-
16% of the activity with thymidine
-
-
?
cytidine + ATP
cytidine 5'-phosphate + ADP
16% of the activity with thymidine
-
-
?
guanosine + ATP
guanosine 5'-phosphate + ADP
-
1.18% of the activity with thymidine
-
-
?
guanosine + ATP
guanosine 5'-phosphate + ADP
1.18% of the activity with thymidine
-
-
?
thymidine + ATP
thymidine 5'-phosphate + ADP
-
-
-
-
?
uridine + ATP
uridine 5'-phosphate + ADP
-
6.45% of the activity with thymidine
-
-
?
uridine + ATP
uridine 5'-phosphate + ADP
6.45% of the activity with thymidine
-
-
?
additional information
?
-
substrate specificity, no activity with cytidine, adenine, guanine, and thymine, no activity with dideoxynucleosides, and with 3'-azido-2',3'-didehydro-3'-deoxythymidine
-
-
?
additional information
?
-
-
substrate specificity, no activity with cytidine, adenine, guanine, and thymine, no activity with dideoxynucleosides, and with 3'-azido-2',3'-didehydro-3'-deoxythymidine
-
-
?
additional information
?
-
the multisubstrate kinase from from Anopheles gambiae (AgdNK) prefers purines
-
-
?
additional information
?
-
substrate binding of non-TK1-like dNKs, overview
-
-
?
additional information
?
-
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
-
-
?
additional information
?
-
-
substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
-
-
?
additional information
?
-
-
substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides
-
-
?
additional information
?
-
the multisubstrate kinase from Bombyx mori (BmdNK) prefers pyrimidines
-
-
?
additional information
?
-
substrate binding of non-TK1-like dNKs, overview
-
-
?
additional information
?
-
-
activity with CTP is 110% of the activity ATP
-
-
?
additional information
?
-
the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed
-
-
?
additional information
?
-
-
the recombinant enzyme preferentially phosphorylates the pyrimidine nucleosides, but phosphorylation of the purine nucleosidesis also efficiently catalyzed
-
-
?
additional information
?
-
-
CTP has the same phosphotransfer capability as ATP
-
-
?
additional information
?
-
-
enzyme evolution by gene duplication of a progenitor kinase
-
-
?
additional information
?
-
enzyme shows broad substrate specificity, this enzyme specificity-type is the earliest in evolution probably encoded by the so-called dCK/dGK/TK2-like gene, several duplications of the progenitor gene are the reason for evolutionary occurring of nucleoside kinases with strict substrate specificities in other organism, phylogenetic tree, insects lost all but 1 nucleotide kinase, overview
-
-
?
additional information
?
-
-
amino acid residues responsible for substrate specificity are V84, M88, and A110, wild-type enzyme shows broad substrate specificity preferring pyrimidines to purines, substrate specificities of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
enzyme shows broad substrate specificity involving V84, M88, and A110
-
-
?
additional information
?
-
-
no activity with adenosine-3'-deoxy-3'-fluoroxylo-2'-triphosphate and adenosine-2'-deoxy-3'-fluoroara-3'-triphosphate as phosphate donors
-
-
?
additional information
?
-
-
no activity with diphosphate, or triphosphate as phosphate donors
-
-
?
additional information
?
-
-
substrate specificities of wild-type and mutant enzymes, overview, no or poor activity with ganciclovir
-
-
?
additional information
?
-
the enzyme shows broad substrate specificity probably due to a wide substrate binding cleft
-
-
?
additional information
?
-
-
dNK plays a role in cell proliferation in physiological conditions
-
-
?
additional information
?
-
-
transient expression of Drosophila melanogaster deoxynucleoside kinase gene in HeLa cells enhances cytotoxicity of nucleoside analogs
-
-
?
additional information
?
-
the multisubstrate kinase from Drosophila prefers pyrimidines
-
-
?
additional information
?
-
Dm-dNK phosphorylates a broad range of substrates including analogues of both purine and pyrimidine nucleosides, and exhibits a higher activity than other nucleoside kinases
-
-
?
additional information
?
-
-
Dm-dNK phosphorylates a broad range of substrates including analogues of both purine and pyrimidine nucleosides, and exhibits a higher activity than other nucleoside kinases
-
-
?
additional information
?
-
enzyme DmdNK is able to phosphorylate all four natural 2'-deoxyribonucleosides with high efficiency, it shows broad specificity and high turnover towards the naturally occurring 2'-deoxyribonucleosides and a similar broad specificity towards nucleoside analogues used in chemotherapy, substrate specificity towards natural and non-natural nucleosides, overview
-
-
?
additional information
?
-
substrate binding of non-TK1-like dNKs, overview
-
-
?
additional information
?
-
-
CTP has the same phosphotransfer capability as ATP
-
-
?
additional information
?
-
-
activity with CTP is 110% of the activity ATP
-
-
?
additional information
?
-
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
-
-
?
additional information
?
-
-
substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine
-
-
?
