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2.7.1.145: deoxynucleoside kinase

This is an abbreviated version!
For detailed information about deoxynucleoside kinase, go to the full flat file.

Word Map on EC 2.7.1.145

Reaction

ATP
+
a 2'-deoxyribonucleoside
=
ADP
+
a 2'-deoxyribonucleoside 5'-phosphate

Synonyms

AGCK, AgdNK, BmdNK, D. melanogaster deoxynucleoside kinase, deoxyribonucleoside kinase, deoxyribonucleoside kinase , deoxyribonucleoside phosphotransferase, Dm-dNK, DmdNK, dNK, kinase (phosphorylating), deoxynucleoside, More, ms-dNK, multi-substrate deoxyribonucleoside kinase, multifunctional deoxynucleoside kinase, multispecific deoxynucleoside kinase, multisubstrate deoxyribonucleoside kinase, nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase, pyrimidine deoxyribonucleoside kinase, white stripe leaf8, WSL8, Xen-PyK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.145 deoxynucleoside kinase

Cloned

Cloned on EC 2.7.1.145 - deoxynucleoside kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
deoxynucleoside kinase gene is introduced into HeLa cells with cationic lipids, to allow its transient expression. Cytotoxicities of 1-beta-D-arabinofuranosylcytosine, 5-fluorodeoxyuridine, and 1-(2-deoxy-2-methylene-beta-D-erythro-pentofuranosyl)cytosine are increased by the deoxynucleoside kinase gene. The combination of the transient expression of the Drosophila deoxynucleoside kinase gene and these nucleoside analogs is a candidate for suicide gene therapy
-
DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895
DNA and amino acid sequence determination and analysis, overexpression in TK-deficient Escherichia coli strain KY895 as GST-fusion protein
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli K-12 cells
-
expressed in human keloid fibroblasts
expressed in MDA-MB-231 and MDA-MB-231R cells
-
expression in a thymidine kinase 1-deficient osteosarcoma cell line. Mitochondrial targeting of Dm-dNK facilitates nucleoside and nucleoside analog phosphorylation and could be used as a strategy to enhance the efficacy of nucleoside analog phosphorylation and concomitantly their cytostatic potential
-
expression in Escherichia coli
expression in HeLa cells from replicating plasmid, using pYK-CMV-luc, pCMV-dNK-T, and pCMV-dNK, is highly cytotoxic and causes cell death without help of nucleoside analogues. Caspase 3/7 activities are not enhanced by transfection of the Dm-dNK gene on a replicating plasmid
-
expression in osteosarcoma cell line
-
expression of the wild-type and mutant enzymes in enzyme-deficient Escherichia coli strain KY895
-
expression of wild-type and mutant enzymes as glutathione-S-transferase fusion proteins in Escherichia coli
-
expression of wild-type and mutant enzymes in Escherichia coli as GST-fusion proteins
-
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 as C-terminally His8-tagged proteins
-
gemcitabine resistance due to deoxycytidine kinase deficiency can be reverted by fruitfly deoxynucleoside kinase, DmdNK, in human uterine sarcoma cells
-
gene dnk, isolated from a phage library, DNA and amino acid sequence determination and analysis, recombinant expression of randomly mutated genes for deoxyribonucleoside kinase in Escherichia coli strain BL21, recombinant expression of catalytically active His10-tagged mutant 4 enzyme in Spodoptera frugiperda SF9 cells
heterologous expression of Drosophila melanogaster enzyme Dm-dNK increases the sensitivity of cancer cells to several cytotoxic nucleoside analogues, recombinant expression of GFP-tagged mutant and wild-type enzymes in TK-1-deficient osteosarcoma cells. dThd kinase activities increased about 40fold in the cells expressing the nuclear dNK-GFP and about 35fold in the cells expressing the mitochondrial mito-dNK-GFP, compared with the untransduced parent cells
overexpression of wild-type and mutant enzyme fused to GFP in cancer cell lines in the nucleus and cytosol, respectively
-
the enzyme with a deletion of the last 20 amino acid residues is expressed in MCF-7-R cells
upon expression in Escherichia coli, enzyme sensitizes cells against various nucleic acid analogs such as gemcitabine
-
wild-type and mutant enzymes, expression in Escherichia coli
-
wild-type and truncated enzyme forms with removal of 10, 20 or 30 C-terminal amino acids, expression as a fusion protein with glutathione S-transferase