Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21 | Bombyx mori |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli | Xenopus laevis |
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21 | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dCTP | competitive versus ATP, feedback inhibition | Xenopus laevis | |
dTTP | competitive versus ATP, feedback inhibition | Bombyx mori | |
dTTP | competitive versus ATP, feedback inhibition | Drosophila melanogaster | |
dTTP | competitive versus ATP, feedback inhibition | Xenopus laevis | |
additional information | no inhibition by dNTPs other than dTTP | Bombyx mori | |
additional information | no inhibition by dNTPs other than dTTP | Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Bombyx mori | |
additional information | - |
additional information | kinetics | Xenopus laevis | |
0.0014 | - |
ATP | - |
Bombyx mori | |
0.0025 | - |
ATP | - |
Xenopus laevis | |
0.0061 | - |
2'-deoxycytidine | - |
Bombyx mori | |
0.0153 | - |
2'-deoxythymidine | - |
Bombyx mori | |
0.031 | - |
2'-deoxycytidine | - |
Xenopus laevis | |
0.138 | - |
2'-deoxythymidine | - |
Xenopus laevis | |
0.429 | - |
2'-deoxyguanosine | - |
Bombyx mori | |
0.501 | - |
2'-deoxyadenosine | - |
Bombyx mori | |
1.2 | - |
2'-deoxyguanosine | - |
Xenopus laevis | |
2.1 | - |
2'-deoxyadenosine | - |
Xenopus laevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | no sorting signals in the amino acid sequence | Bombyx mori | - |
- |
nucleus | enzyme possesses 2 potential nuclear targeting sequences | Drosophila melanogaster | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bombyx mori | |
Mg2+ | - |
Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26300 | - |
2 * 26300, recombinant enzyme, SDS-PAGE | Xenopus laevis |
26900 | - |
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
29100 | - |
2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
31300 | - |
2 * 31300, recombinant enzyme, SDS-PAGE | Bombyx mori |
44600 | - |
recombinant N-terminally truncated enzyme, gel filtration | Drosophila melanogaster |
46000 | - |
recombinant enzyme, native PAGE | Xenopus laevis |
48500 | - |
recombinant full length enzyme, gel filtration | Drosophila melanogaster |
49000 | - |
recombinant N-terminally truncated enzyme, native PAGE | Drosophila melanogaster |
49800 | - |
recombinant enzyme, gel filtration | Xenopus laevis |
57700 | - |
recombinant full length enzyme, native PAGE | Drosophila melanogaster |
64400 | - |
recombinant enzyme, gel filtration | Bombyx mori |
69300 | - |
recombinant enzyme, native PAGE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxycytidine | Xenopus laevis | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | Xenopus laevis | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | Q9BKL3 | putative | - |
Drosophila melanogaster | - |
- |
- |
Xenopus laevis | Q8UVZ9 | putative | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography | Bombyx mori |
recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin | Drosophila melanogaster |
recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
substrate specificity | Bombyx mori |
additional information | - |
substrate specificity | Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-beta-D-arabinofuranosylcytosine | - |
Bombyx mori | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosylcytosine | - |
Xenopus laevis | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosylthymine | - |
Bombyx mori | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
ATP + 1-beta-D-arabinofuranosylthymine | - |
Xenopus laevis | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
ATP + 2',3'-didehydro-3'-deoxythymidine | - |
Bombyx mori | ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2',3'-dideoxycytidine | - |
Bombyx mori | ADP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
ATP + 2',3'-dideoxycytidine | low activity | Xenopus laevis | ADP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
ATP + 2',3'-dideoxythymidine | - |
Bombyx mori | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 2',3'-dideoxythymidine | low activity | Xenopus laevis | ADP + 2',3'-dideoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Bombyx mori | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyadenosine | - |
Xenopus laevis | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Bombyx mori | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxycytidine | - |
Xenopus laevis | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Bombyx mori | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxyguanosine | - |
Xenopus laevis | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | - |
Xenopus laevis | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | best substrate | Xenopus laevis | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxythymidine | high activity | Bombyx mori | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2-chloro-2'-deoxyadenosine | low activity | Xenopus laevis | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 2-chloro-2'-deoxyadenosine | high activity | Bombyx mori | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine | low activity | Bombyx mori | ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine | low activity | Xenopus laevis | ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 3'-deoxyadenosine | - |
Bombyx mori | ADP + 3'-deoxyadenosine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | high activity | Bombyx mori | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | high activity | Xenopus laevis | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyladenine | - |
Bombyx mori | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
ATP + 9-beta-D-arabinofuranosyladenine | very low activity | Xenopus laevis | ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
ATP + aciclovir | - |
Bombyx mori | ADP + aciclovir phosphate | - |
? | |
ATP + cytidine | - |
Bombyx mori | ADP + cytidine 5'-phosphate | - |
? | |
ATP + cytidine | very low activity | Xenopus laevis | ADP + cytidine 5'-phosphate | - |
? | |
ATP + deoxyuridine | best substrate | Bombyx mori | ADP + deoxyuridine 5'-phosphate | - |
? | |
ATP + deoxyuridine | high activity | Xenopus laevis | ADP + deoxyuridine 5'-phosphate | - |
? | |
ATP + fludarabine | - |
Bombyx mori | ADP + fludarabine phosphate | - |
? | |
ATP + stavudine | anti-HIV nucleoside analogue | Bombyx mori | ADP + stavudine phosphate | - |
? | |
ATP + uridine | very low activity | Xenopus laevis | ADP + uridine 5'-phosphate | - |
? | |
ATP + uridine | low activity | Bombyx mori | ADP + uridine 5'-phosphate | - |
? | |
additional information | substrate specificity | Drosophila melanogaster | ? | - |
? | |
additional information | substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine | Bombyx mori | ? | - |
? | |
additional information | substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine | Xenopus laevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26300, recombinant enzyme, SDS-PAGE | Xenopus laevis |
dimer | 2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE | Drosophila melanogaster |
dimer | 2 * 31300, recombinant enzyme, SDS-PAGE | Bombyx mori |
More | quarternary structure determination | Bombyx mori |
More | quarternary structure determination | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
dNK | - |
Drosophila melanogaster |
dNK | - |
Bombyx mori |
More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Drosophila melanogaster |
More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Bombyx mori |
More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Xenopus laevis |
multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
multisubstrate deoxyribonucleoside kinase | - |
Bombyx mori |
pyrimidine deoxyribonucleoside kinase | - |
Xenopus laevis |
Xen-PyK | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bombyx mori | |
ATP | - |
Xenopus laevis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Drosophila melanogaster | |
additional information | - |
additional information | inhibition kinetics, overview | Bombyx mori | |
additional information | - |
additional information | inhibition kinetics, overview | Xenopus laevis |