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5.84 - 12.07
2-methoxyphenylpyruvate
0.77 - 0.86
2-oxo-4-phenyl-butanoic acid
0.007 - 6.9
2-oxoglutarate
1.01 - 10.32
3,4-dimethoxyphenylpyruvate
-
4.92 - 11.56
3-methoxyphenylpyruvate
4.12 - 10.14
4-methoxyphenylpyruvate
29.4
kynurenine
at pH 7.4 and 37°C
4.1 - 20.3
L-2-amino-4-methoxy-4-oxobutanoic acid
29
L-Asp
pH 8, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S
2.05
L-cysteine sulfinic acid
-
pH 8.5, 60°C, recombinant enzyme
0.724
L-kynurenine
-
at pH 7.4
0.3
L-Phe
pH 8, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S
1.1 - 6.6
L-phenylalanine
0.92 - 4.24
phenylpyruvate
0.00025
pyridoxyl 5'-phosphate
-
pH 7.6, 25°C
additional information
additional information
-
5.84
2-methoxyphenylpyruvate
-
mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C
12.07
2-methoxyphenylpyruvate
-
wild type enzyme, at pH 8.0 and 40°C
0.77
2-oxo-4-phenyl-butanoic acid
-
wild-type enzyme
0.86
2-oxo-4-phenyl-butanoic acid
-
mutant enzyme R292E/L18H
0.007
2-oxoglutarate
-
recombinant deletion mutant, pH 7.5, 25°C
0.062
2-oxoglutarate
-
30°C
0.07
2-oxoglutarate
-
isozyme pAspAT, gamma-form, pH 8.0, 25°C
0.071
2-oxoglutarate
-
pH 7.6, 25°C
0.075
2-oxoglutarate
-
isozyme pAspAT, beta-form, pH 8.0, 25°C
0.08
2-oxoglutarate
-
recombinant mutant C166S, pH 7.5, 25°C
0.08
2-oxoglutarate
-
isozyme Asp-DEAE-1, pH 8.0
0.087
2-oxoglutarate
-
isozyme pAspAT, alpha-form, pH 8.0, 25°C
0.088 - 0.095
2-oxoglutarate
-
pH 7.4, 30°C
0.093
2-oxoglutarate
-
cytosolic isozyme, pH 7.4, 25°C
0.098
2-oxoglutarate
-
mitochondrial isozyme, pH 7.4, 25°C
0.105
2-oxoglutarate
-
isoenzyme AAT-2, pH 7.8, 37°C
0.11
2-oxoglutarate
-
pH 8.3, 25°C
0.11
2-oxoglutarate
-
isoenzyme AAT-1, pH 7.8, 37°C
0.12
2-oxoglutarate
-
isozyme Asp-DEAE-2, pH 8.0
0.13
2-oxoglutarate
-
isozyme mAspAT, pH 8.0, 25°C
0.13
2-oxoglutarate
allozyme AAT-2b, pH 7.5, 25°C
0.13
2-oxoglutarate
allozyme AAT-2c, pH 7.5, 25°C
0.13
2-oxoglutarate
allozyme AAT-3a, pH 7.5, 25°C
0.14
2-oxoglutarate
-
isozyme cAspAT, beta-form, pH 8.0, 25°C
0.17
2-oxoglutarate
-
pH 7.4, 25°C
0.17
2-oxoglutarate
-
isozyme cAspAT, alpha-form, pH 8.0, 25°C
0.17
2-oxoglutarate
allozyme AAT-2a, pH 7.5, 25°C
0.18
2-oxoglutarate
allozyme AAT-3c, pH 7.5, 25°C
0.2
2-oxoglutarate
-
pH 7.5, 37°C
0.2
2-oxoglutarate
-
pH 8.0, 45°C
0.2
2-oxoglutarate
-
isozyme AAT-P2, pH 7.6, 25°C
0.2
2-oxoglutarate
-
isozyme AspAT-3, 25°C, pH 8.0
0.2
2-oxoglutarate
-
isozyme cAspAT, gamma-form, pH 8.0, 25°C
0.22
2-oxoglutarate
-
pH 8.5, 60°C, recombinant enzyme
0.23
2-oxoglutarate
-
isozyme AspAT-1, 25°C, pH 8.0
0.24
2-oxoglutarate
-
25°C, pH 8.0
0.25
2-oxoglutarate
allozyme AAT-3b, pH 7.5, 25°C
0.26
2-oxoglutarate
-
isozyme AAT-P1, pH 7.6, 25°C
0.3
2-oxoglutarate
pH 8.0, 45°C
0.3
2-oxoglutarate
-
recombinant isozyme mAspAT, pH 7.5, 25°C
0.3
2-oxoglutarate
-
cytosolic isozyme, pH 8.0, 37°C
0.33
2-oxoglutarate
-
recombinant premature isozyme pmAspAT, pH 7.5, 25°C
0.39
2-oxoglutarate
-
isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0
0.46
2-oxoglutarate
pH 7.5, 25°C, recombinant mutant T84V
0.48
2-oxoglutarate
pH 8, cosubstrate: L-Phe, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S
0.5
2-oxoglutarate
-
pH 7.5, 37°C
0.55
2-oxoglutarate
-
pH 7.8, 25°C
0.55
2-oxoglutarate
-
isozyme AAT-1, pH 8.0
0.59
2-oxoglutarate
-
wild-type, pH 8.4, 25°C
0.67
2-oxoglutarate
-
isozyme AST II, 37°C, pH 7.5
0.8
2-oxoglutarate
-
recombinant mutant C166A, pH 7.5, 25°C
1
2-oxoglutarate
pH 7.5, 25°C, recombinant mutant E108K
1.1
2-oxoglutarate
pH 7.5, 25°C, recombinant wild-type enzyme
1.2
2-oxoglutarate
pH 7.5, 25°C, recombinant mutant A168G
1.25
2-oxoglutarate
-
cytoplasmic enzyme
1.33
2-oxoglutarate
-
cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
1.4
2-oxoglutarate
-
pH 7.5, 35°C
1.47
2-oxoglutarate
-
enzyme from nonsynaptic mitochondrion, pH 7.8, 30°C
1.5
2-oxoglutarate
pH 7.5, 37°C
1.7
2-oxoglutarate
-
cytosolic isozyme
1.75
2-oxoglutarate
-
mitochondrial enzyme
1.95
2-oxoglutarate
-
enzyme from synaptic mitochondrion, pH 7.8, 30°C
1.95
2-oxoglutarate
-
mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
2.06
2-oxoglutarate
-
cytosolic isozyme, pH 6.8, 37°C
2.2
2-oxoglutarate
-
mitochondrial isozyme
2.4
2-oxoglutarate
wild-type, pH 8.0, 25°C
2.5
2-oxoglutarate
using L-aspartate as a cosubstrate, at pH 8.0 and 60°C
2.6
2-oxoglutarate
-
pH 8.0, 50°C
2.6
2-oxoglutarate
pH 8, cosubstrate: L-Asp, mutant enzyme A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297S
3.2
2-oxoglutarate
pH 7.5, 37°C
6.9
2-oxoglutarate
-
mitochondrial isozyme, pH 8.0, 37°C
1.01
3,4-dimethoxyphenylpyruvate
-
mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C
-
10.32
3,4-dimethoxyphenylpyruvate
-
wild type enzyme, at pH 8.0 and 40°C
-
4.92
3-methoxyphenylpyruvate
-
mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C
11.56
3-methoxyphenylpyruvate
-
wild type enzyme, at pH 8.0 and 40°C
4.12
4-methoxyphenylpyruvate
-
mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C
10.14
4-methoxyphenylpyruvate
-
wild type enzyme, at pH 8.0 and 40°C
4.1
L-2-amino-4-methoxy-4-oxobutanoic acid
-
isozyme AAT2, 25°C
20.3
L-2-amino-4-methoxy-4-oxobutanoic acid
-
isozyme AAT1, 25°C
3.5
L-alanine
-
pH 7.5, 37°C
0.039
L-aspartate
at pH 7.4 and 30°C
0.09
L-aspartate
-
recombinant deletion mutant, pH 7.5, 25°C
0.108
L-aspartate
at pH 7.4 and 30°C
0.25
L-aspartate
-
recombinant premature isozyme pmAspAT, pH 7.5, 25°C
0.29
L-aspartate
-
recombinant mutant C166A, pH 7.5, 25°C
0.33
L-aspartate
-
recombinant mutant C166S, pH 7.5, 25°C
0.35
L-aspartate
-
cytoplasmic enzyme
0.36
L-aspartate
-
recombinant isozyme mAspAT, pH 7.5, 25°C
0.4
L-aspartate
-
mitochondrial isozyme, pH 7.4, 25°C
0.43
L-aspartate
-
cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
0.5
L-aspartate
-
isozyme Asp-DEAE-2, pH 8.0
0.51
L-aspartate
-
mitochondrial isozyme
0.75
L-aspartate
-
mitochondrial enzyme
0.78
L-aspartate
-
mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
0.85
L-aspartate
-
isozyme Asp-DEAE-1, pH 8.0
1.2
L-aspartate
-
isozyme AAT-1, pH 8.0
1.3
L-aspartate
-
isozyme mAspAT, pH 8.0, 25°C
1.3
L-aspartate
-
25°C, pH 8.0
1.5
L-aspartate
-
isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0
1.5
L-aspartate
at pH 8.0 and 60°C
1.6
L-aspartate
pH 7.5, 25°C, recombinant mutant T84V
1.7
L-aspartate
-
isozyme AspAT-3, 25°C, pH 8.0
1.7
L-aspartate
wild-type, pH 8.0, 25°C
1.9
L-aspartate
-
wild-type, pH 8.4, 25°C
1.9
L-aspartate
-
mitochondrial isozyme, pH 8.0, 37°C
1.9
L-aspartate
pH 7.5, 25°C, recombinant wild-type enzyme
2
L-aspartate
-
pH 7.4, 25°C
2.1
L-aspartate
pH 7.5, 37°C
2.1 - 2.9
L-aspartate
-
pH 7.4, 30°C
2.2
L-aspartate
-
isozyme AAT-P1, pH 7.6, 25°C
2.2
L-aspartate
-
isozyme pAsp-AT, beta-form, pH 8.0, 25°C
2.3
L-aspartate
-
pH 8.3, 25°C
2.3
L-aspartate
-
isozyme pAsp-AT, gamma-form, pH 8.0, 25°C
2.34
L-aspartate
at pH 7.4 and 37°C
2.4
L-aspartate
-
isoenzyme AAT-1, pH 7.8, 37°C
2.53
L-aspartate
-
pH 7.8, 25°C
2.6
L-aspartate
-
isozyme AAT-P2, pH 7.6, 25°C
2.6
L-aspartate
-
isozyme pAsp-AT and cAspAT, alpha-form, pH 8.0, 25°C
2.8
L-aspartate
pH 7.5, 25°C, recombinant mutant E108K
3
L-aspartate
-
cytosolic isozyme, pH 7.4, 25°C
3
L-aspartate
-
isozyme cAspAT, gamma-form, pH 8.0, 25°C
3
L-aspartate
-
pH 8.0, 50°C
3.1
L-aspartate
pH 7.5, 25°C, mutant I33Q/Y214Q/R280Y
3.2
L-aspartate
-
isozyme cAspAT, beta-form, pH 8.0, 25°C
3.7
L-aspartate
-
pH 7.5, 37°C
3.7
L-aspartate
-
isoenzyme AAT-2, pH 7.8, 37°C
3.8
L-aspartate
pH 7.5, 25°C, recombinant mutant A168G
3.9
L-aspartate
-
cytosolic isozyme, pH 8.0, 37°C
4
L-aspartate
pH 7.5, 25°C, wild-type
4.6
L-aspartate
-
pH 7.5, 35°C
5
L-aspartate
-
pH 8.0, 45°C
6.5
L-aspartate
-
cytosolic isozyme
6.7
L-aspartate
pH 8.0, 45°C
6.8
L-aspartate
pH 7.5, 37°C
7.1
L-aspartate
allozyme AAT-3c, pH 7.5, 25°C
7.5
L-aspartate
-
isozyme AspAT-1, 25°C, pH 8.0
8.3
L-aspartate
allozyme AAT-2a, pH 7.5, 25°C
8.5
L-aspartate
allozyme AAT-2c, pH 7.5, 25°C
9.3
L-aspartate
allozyme AAT-3a, pH 7.5, 25°C
9.9
L-aspartate
allozyme AAT-2b, pH 7.5, 25°C
10
L-aspartate
allozyme AAT-3b, pH 7.5, 25°C
15.1
L-aspartate
-
isozyme AST II, 37°C, pH 7.5
22.5
L-aspartate
-
cytosolic isozyme, pH 6.8, 37°C
26
L-aspartate
pH 7.5, 37°C
37
L-aspartate
pH 7.5, 37°C
8.57
L-cysteine
at pH 7.4 and 30°C
20.62
L-cysteine
at pH 7.4 and 30°C
0.5
L-glutamate
-
cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
0.58
L-glutamate
-
mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
0.75
L-glutamate
-
isozyme Asp-DEAE-2, pH 8.0
1.6
L-glutamate
-
isozyme Asp-DEAE-1, pH 8.0
3.88
L-glutamate
-
pH 7.8, 25°C
5.2
L-glutamate
pH 7.5, 25°C, mutant I33Q/Y214Q/R280Y
5.7
L-glutamate
-
pH 8.0, 45°C
7.5
L-glutamate
-
isozyme mAspAT, pH 8.0, 25°C
8
L-glutamate
pH 8.0, 45°C
8.2
L-glutamate
allozyme AAT-2b, pH 7.5, 25°C
8.3
L-glutamate
allozyme AAT-3a, pH 7.5, 25°C
12
L-glutamate
-
isozyme AAT-P2, pH 7.6, 25°C
12
L-glutamate
allozyme AAT-2a, pH 7.5, 25°C
12.2
L-glutamate
-
isozyme AspAT-3, 25°C, pH 8.0
13
L-glutamate
-
pH 8.0, 25°C
13
L-glutamate
-
isozyme cAspAT, gamma-form, pH 8.0, 25°C
13.2
L-glutamate
-
isoenzyme AAT-2
14
L-glutamate
-
isozyme cAspAT, beta-form, pH 8.0, 25°C
14
L-glutamate
allozyme AAT-3c, pH 7.5, 25°C
15
L-glutamate
-
25°C, pH 8.0
16
L-glutamate
allozyme AAT-2c, pH 7.5, 25°C
17
L-glutamate
-
isozyme cAspAT, alpha-form, pH 8.0, 25°C
17
L-glutamate
allozyme AAT-3b, pH 7.5, 25°C
17.4
L-glutamate
-
isozyme AspAT-1, 25°C, pH 8.0
18
L-glutamate
-
isozyme pAspAT, gamma-form, pH 8.0, 25°C
18.5
L-glutamate
-
isozyme AAT-1, pH 8.0
19.4
L-glutamate
-
isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0
20
L-glutamate
-
isozyme pAspAT, beta-form, pH 8.0, 25°C
22
L-glutamate
-
isozyme AAT-P1, pH 7.6, 25°C
32
L-glutamate
-
isozyme pAspAT, alpha-form, pH 8.0, 25°C
37
L-glutamate
pH 7.5, 25°C, wild-type
105
L-Lys
-
mutant enzyme R292E/L18H
189
L-Lys
-
wild-type enzyme
6.9
L-methionine
pH 7.5, 37°C
56
L-methionine
pH 7.5, 37°C
1.1
L-phenylalanine
pH 7.5, 37°C
6.6
L-phenylalanine
pH 7.5, 37°C
2.3
L-tryptophan
pH 7.5, 37°C
5
L-tryptophan
-
pH 7.6, 25°C
5
L-tryptophan
-
L-glutamate, isoenzyme AAT-1
1.43
L-tyrosine
-
pH 7.6, 25°C
1.5
L-tyrosine
pH 7.5, 37°C
1.6
L-tyrosine
pH 7.5, 37°C
0.01
oxaloacetate
-
25°C, pH 8.0
0.017
oxaloacetate
pH 8.0, 30°C
0.02
oxaloacetate
-
isozyme AAT-P2, pH 7.6, 25°C
0.023
oxaloacetate
-
isozyme pAspAT, gamma-form, pH 8.0, 25°C
0.027
oxaloacetate
-
isozyme AAT-2, including forms 2a, 2b and 2c, pH 8.0
0.03
oxaloacetate
-
isozyme pAspAT, alpha-form, pH 8.0, 25°C
0.031
oxaloacetate
-
isozyme AAT-1, pH 8.0
0.032
oxaloacetate
-
pH 8.0, 45°C
0.033
oxaloacetate
-
isozyme pAspAT, beta-form, pH 8.0, 25°C
0.037
oxaloacetate
allozyme AAT-3a, pH 7.5, 25°C
0.039
oxaloacetate
allozyme AAT-2b, pH 7.5, 25°C
0.043
oxaloacetate
-
isoenzyme AAT-1
0.043
oxaloacetate
allozyme AAT-3c, pH 7.5, 25°C
0.044
oxaloacetate
-
pH 7.8, 25°C
0.045
oxaloacetate
-
isozyme Asp-DEAE-2, pH 8.0
0.048
oxaloacetate
-
isoenzyme AAT-2
0.049
oxaloacetate
-
pH 8.0, 25°C
0.052
oxaloacetate
allozyme AAT-2a, pH 7.5, 25°C
0.056
oxaloacetate
-
isozyme mAspAT, pH 8.0, 25°C
0.057
oxaloacetate
allozyme AAT-2c, pH 7.5, 25°C
0.057
oxaloacetate
allozyme AAT-3b, pH 7.5, 25°C
0.065
oxaloacetate
-
isozyme cAspAT, alpha-form, pH 8.0, 25°C
0.08
oxaloacetate
-
isozyme cAspAT, gamma-form, pH 8.0, 25°C
0.085
oxaloacetate
-
isozyme cAspAT, beta-form, pH 8.0, 25°C
0.1
oxaloacetate
-
isozyme Asp-DEAE-1, pH 8.0
0.1
oxaloacetate
-
isozyme AAT-P1, pH 7.6, 25°C
0.2
oxaloacetate
-
isozyme AspAT-1, 25°C, pH 8.0
0.32
oxaloacetate
-
isozyme AspAT-3, 25°C, pH 8.0
0.37
oxaloacetate
-
pH 7.6, 25°C
0.6
oxaloacetate
pH 8.0, 45°C
2.04
oxaloacetate
-
mitochondrial enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 1.6 mM L-aspartate, 4 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
2.45
oxaloacetate
-
cytoplasmic enzyme, in 0.05 M potassium phosphate buffer (pH 7.5) containing 0.9 mM L-aspartate, 2.7 mM 2-oxoglutarate, 0.1 mM NADH, and 1 U/ml malate dehydrogenase
0.92
phenylpyruvate
-
mutant enzyme N165Y/R316H/A381T/P399L, at pH 8.0 and 40°C
4.24
phenylpyruvate
-
wild type enzyme, at pH 8.0 and 40°C
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
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additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
Leptosphaeria michotii
-
-
-
additional information
additional information
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-
-
additional information
additional information
dolphin
-
-
-
additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
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additional information
additional information
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kinetics
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additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
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additional information
additional information
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kinetics
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additional information
additional information
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kinetics
-
additional information
additional information
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kinetics
-
additional information
additional information
kinetics
-
additional information
additional information
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kinetics
-
additional information
additional information
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recombinant enzyme
-
additional information
additional information
-
mutants
-
additional information
additional information
-
recombinant enzyme, high temperature dependence of Km
-
additional information
additional information
-
wild-type and mutants
-
additional information
additional information
-
wild-type and mutants
-
additional information
additional information
wild-type and mutants
-
additional information
additional information
-
wild-type and mutants
-
additional information
additional information
steady-state kinetics analysis
-
additional information
additional information
-
steady-state kinetics analysis
-
2.6.1.1 aspartate transaminase
results (
results (
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