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Literature summary for 2.6.1.1 extracted from

  • Martins, L.L.; Mourato, M.P.; de Varennes, A.
    Effects of substrate structural analogues on the enzymatic activities of aspartate aminotransferase isoenzymes (2001), J. Enzyme Inhib., 16, 251-257.
    View publication on PubMed
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
fumarate competitive Lupinus albus
Glutarate isozyme AAT1: competitive Lupinus albus
L-2-amino-5-methoxy-5-oxopentanoic acid only isozyme AAT2, competitive against L-asparate Lupinus albus
L-cysteic acid competitive Lupinus albus
malate competitive Lupinus albus
Maleate
-
 Lupinus albus
succinate competitive Lupinus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1
-
 L-2-amino-4-methoxy-4-oxobutanoic acid isozyme AAT2, 25°C Lupinus albus
20.3
-
 L-2-amino-4-methoxy-4-oxobutanoic acid isozyme AAT1, 25°C Lupinus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Lupinus albus enzyme plays an important role in numerous metabolic processes oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Lupinus albus
-
 cv. Estoril
-
Lupinus albus
-
 2 isozymes: AAT1 and AAT2
-

Purification (Commentary)

Purification (Comment) Organism
isozymes AAT1 and AAT2 Lupinus albus

Reaction

Reaction Comment Organism Reaction ID
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate bi bi ping pong reaction kinetic Lupinus albus
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf isozymes AAT1 and AAT2 Lupinus albus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Lupinus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-amino-4-methoxy-4-oxobutanoic acid + 2-oxoglutarate isozymes AAT1 and AAT2, the latter shows lower activity Lupinus albus 4-methoxy-2,4-dioxobutanoic acid + L-glutamate
-
 ?
L-2-amino-4-methoxy-4-oxobutanoic acid + oxaloacetate isozymes AAT1 and AAT2, low activity Lupinus albus 4-methoxy-2,4-dioxobutanoic acid + L-aspartate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Lupinus albus oxaloacetate + L-glutamate
-
 r
L-aspartate + 2-oxoglutarate enzyme plays an important role in numerous metabolic processes Lupinus albus oxaloacetate + L-glutamate
-
 ?
L-cysteic acid + 2-oxoglutarate no activity Lupinus albus 2-oxo-3-sulfopropionate + L-glutamate
-
 ?
additional information substrate specificity Lupinus albus ?
-
 ?
additional information poor substrates are: glyoxylic acid, pyruvate, succinate, maleate, L-asparagine, L-glutamine, L-alanine, glycine Lupinus albus ?
-
 ?
additional information no activity with L-2-amino-5-methoxy-5-oxopentanoic acid, fumarate, hydroxypyruvate, glutarate, L-proline, L-arginine, and L-lysine Lupinus albus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Lupinus albus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.21
-
 Maleate isozyme AAT2, versus 2-oxoglutarate, 25°C Lupinus albus
1.4
-
 Maleate isozyme AAT1, versus 2-oxoglutarate, 25°C Lupinus albus
6.78
-
 fumarate isozyme AAT2, 25°C, versus 2-oxoglutarate Lupinus albus
8.5
-
 Maleate isozyme AAT2, versus L-aspartate, 25°C Lupinus albus
8.86
-
 succinate isozyme AAT2, 25°C, versus 2-oxoglutarate Lupinus albus
10.58
-
 Maleate AAT1, versus L-aspartate, 25°C Lupinus albus
10.66
-
 succinate isozyme AAT1, 25°C, versus 2-oxoglutarate Lupinus albus
15.05
-
 Glutarate isozyme AAT1, 25°C, versus 2-oxoglutarate Lupinus albus
16.29
-
 L-cysteinic acid isozyme AAT1, 25°C, versus 2-oxoglutarate Lupinus albus
16.84
-
 succinate isozyme AAT2, 25°C, versus L-aspartate Lupinus albus
20.73
-
 L-cysteinic acid isozyme AAT2, 25°C, versus 2-oxoglutarate Lupinus albus
24.59
-
 fumarate isozyme AAT1, 25°C, versus 2-oxoglutarate Lupinus albus
25.85
-
 L-2-amino-5-methoxy-5-oxopentanoic acid isozyme AAT2, 25°C, versus 2-oxoglutarate Lupinus albus
26.55
-
 Glutarate isozyme AAT1, 25°C, versus L-aspartate Lupinus albus
33.72
-
 fumarate isozyme AAT2, 25°C, versus L-aspartate Lupinus albus
36.89
-
 succinate isozyme AAT1, 25°C, versus L-aspartate Lupinus albus
44.12
-
 fumarate isozyme AAT1, 25°C, versus L-aspartate Lupinus albus
97.63
-
 L-cysteinic acid isozyme AAT1, 25°C, versus L-aspartate Lupinus albus
150.8
-
 L-cysteinic acid isozyme AAT2, 25°C, versus L-aspartate Lupinus albus