Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged protein | Trypanosoma cruzi |
expression in Escherichia coli, His-tagged protein | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
L-phenylalanine | pH 7.5, 37°C | Trypanosoma cruzi | |
1.5 | - |
2-oxoglutarate | pH 7.5, 37°C | Trypanosoma brucei | |
1.5 | - |
L-tyrosine | pH 7.5, 37°C | Trypanosoma cruzi | |
1.6 | - |
L-tyrosine | pH 7.5, 37°C | Trypanosoma brucei | |
2.1 | - |
L-aspartate | pH 7.5, 37°C | Trypanosoma cruzi | |
2.3 | - |
L-tryptophan | pH 7.5, 37°C | Trypanosoma cruzi | |
3.2 | - |
2-oxoglutarate | pH 7.5, 37°C | Trypanosoma cruzi | |
6.6 | - |
L-phenylalanine | pH 7.5, 37°C | Trypanosoma brucei | |
6.8 | - |
L-aspartate | pH 7.5, 37°C | Trypanosoma brucei | |
6.9 | - |
L-methionine | pH 7.5, 37°C | Trypanosoma cruzi | |
26 | - |
L-aspartate | pH 7.5, 37°C | Trypanosoma brucei | |
37 | - |
L-aspartate | pH 7.5, 37°C | Trypanosoma cruzi | |
56 | - |
L-methionine | pH 7.5, 37°C | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | enzyme is constitutively expressed | Trypanosoma brucei | 5829 | - |
cytosol | enzyme is specifically induced in the mammalian stages of Trypanosoma cruzi | Trypanosoma cruzi | 5829 | - |
mitochondrion | enzyme is constitutively expressed | Trypanosoma cruzi | 5739 | - |
mitochondrion | enzyme is down-regulated in bloddstream forms of Trypanosoma brucei | Trypanosoma brucei | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | Q964F0 | mitochondrial isoform | - |
Trypanosoma brucei | Q964F1 | cytosolic isoform | - |
Trypanosoma cruzi | Q4D080 | cytosolic isoform | - |
Trypanosoma cruzi | Q4D1Q4 | mitochondrial isoform | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + 2-oxoglutarate | - |
Trypanosoma cruzi | oxaloacetate + L-glutamate | - |
? | |
L-aspartate + 2-oxoglutarate | - |
Trypanosoma brucei | oxaloacetate + L-glutamate | - |
? | |
L-methionine + 2-oxoglutarate | - |
Trypanosoma cruzi | L-glutamate + 4-methylsulfanyl-2-oxobutyric acid | - |
? | |
L-methionine + 2-oxoglutarate | - |
Trypanosoma brucei | L-glutamate + 4-methylsulfanyl-2-oxobutyric acid | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Trypanosoma cruzi | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
L-phenylalanine + 2-oxoglutarate | - |
Trypanosoma brucei | 2-oxo-3-phenylpropionic acid + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Trypanosoma cruzi | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tryptophan + 2-oxoglutarate | - |
Trypanosoma brucei | 3-indole-2-oxopropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Trypanosoma cruzi | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
L-tyrosine + 2-oxoglutarate | - |
Trypanosoma brucei | 3-(4-hydroxyphenyl)-2-oxopropionic acid + L-glutamate | - |
? | |
additional information | the cytosolic isoform displays broad substrate specificity | Trypanosoma cruzi | ? | - |
? | |
additional information | the cytosolic isoform displays broad substrate specificity | Trypanosoma brucei | ? | - |
? | |
additional information | the mitochondrial isoform is highly specific for substrates L-aspartate and 2-oxoglutarate | Trypanosoma brucei | ? | - |
? | |
additional information | the mitochondrial isoform is highly specific for substrates L-aspartate and 2-oxoglutarate | Trypanosoma cruzi | ? | - |
? |