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Literature summary for 2.6.1.1 extracted from

  • Sung, M.H.; Tanizawa, K.; Tanaka, H.; Kuramitsu, S.; Kagamiyama, H.; Soda, K.
    Purification and characterization of thermostable aspartate aminotransferase from a thermophilic Bacillus species (1990), J. Bacteriol., 172, 1345-1351.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
 2-oxoglutarate pH 8.0, 50°C Bacillus sp. (in: Bacteria)
3
-
 L-aspartate pH 8.0, 50°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 2 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)
97000 98000 gel filtration, sedimentation equilibrium Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Bacillus sp. (in: Bacteria)
-
 oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate Bacillus sp. (in: Bacteria) YM-2
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) YM-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
220
-
-
 Bacillus sp. (in: Bacteria)

Storage Stability

Storage Stability Organism
-20°C, protein concentration above 10 mg/ml Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) oxaloacetate + L-glutamate
-
 ?
L-aspartate + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) YM-2 oxaloacetate + L-glutamate
-
 ?
L-cysteine sulfinic acid + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ir
L-cysteine sulfinic acid + 2-oxoglutarate
-
 Bacillus sp. (in: Bacteria) YM-2 2-oxo-3-sulfinopropionic acid + L-glutamate
-
 ir
L-tryptophan + 2-oxoglutarate 0.1% of the activity with aspartate Bacillus sp. (in: Bacteria) 3-indole-2-oxopropionic acid + L-glutamate
-
 ?
L-tryptophan + 2-oxoglutarate 0.1% of the activity with aspartate Bacillus sp. (in: Bacteria) YM-2 3-indole-2-oxopropionic acid + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 1 h, pH 6.0-11.0, stable Bacillus sp. (in: Bacteria)
80
-
 20 min, 35% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11 37°C, 1 h, stable Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 mol of pyridoxal phosphate per mol of subunit Bacillus sp. (in: Bacteria)