Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.6.1.1 extracted from

  • Orlacchio, A.; Campos-Cavieres, M.; Pashev, I.; Mun, E.A.
    Some kinetic and other properties of the isoenzymes of aspartate aminotransferase isolated from sheep liver (1979), Biochem. J., 177, 583-593.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.6.1.1

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate cytoplasmic enzyme inhibited above 0.25 mM, mitochondrial enzyme not Ovis aries
phosphate inhibition of cofactor binding to the apoenzyme, cytosolic and mitochondrial isozymes Ovis aries
potassium phosphate
-
 Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Ovis aries
0.093
-
 2-oxoglutarate cytosolic isozyme, pH 7.4, 25°C Ovis aries
0.098
-
 2-oxoglutarate mitochondrial isozyme, pH 7.4, 25°C Ovis aries
0.4
-
 L-aspartate mitochondrial isozyme, pH 7.4, 25°C Ovis aries
3
-
 L-aspartate cytosolic isozyme, pH 7.4, 25°C Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Ovis aries 5829
-
mitochondrion
-
 Ovis aries 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87100
-
 sedimentation equilibrium analysis Ovis aries

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-aspartate + 2-oxoglutarate Ovis aries
-
 oxaloacetate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Ovis aries
-
 cytosolic and mitochondrial isozymes
-

Purification (Commentary)

Purification (Comment) Organism
mitochondrial isozyme, 57.3fold Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
151.5
-
 mitochondrial isozyme Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate + 2-oxoglutarate
-
 Ovis aries oxaloacetate + L-glutamate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
 Ovis aries

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate a pyridoxal 5'-phosphate protein Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0195
-
 phosphate mitochondrial isozyme, pH 7.4, 37°C Ovis aries
0.032
-
 phosphate cytosolic isozyme, pH 7.4, 37°C Ovis aries