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2.4.2.8: hypoxanthine phosphoribosyltransferase

This is an abbreviated version!
For detailed information about hypoxanthine phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.8

Reaction

IMP
+
diphosphate
=
hypoxanthine
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

6-hydroxypurine phosphoribosyltransferase, 6-mercaptopurine phosphoribosyltransferase, 6-oxo PRTase, 6-oxopurine phosphoribosyltransferase, 6-oxopurine PRTase, Cthe_2254, EcHPRT, GMP pyrophosphorylase, GPRT, gpT-2, guanine phosphoribosyltransferase, guanine PRTase, guanine-hypoxanthine phosphoribosyltransferase, guanosine 5'-phosphate pyrophosphorylase, guanosine phosphoribosyltransferase, guanylate pyrophosphorylase, guanylic pyrophosphorylase, HGPRT, HGPRT-1, HGPRTase, HGXPRT, HGXPRTase, HG[X]PRT, HPRT, HPRT1, HPRTJerusalem, HPT, HXGPRT, hypoxanthine guanine phosphoribosyltransferase, hypoxanthine guanine xanthine phosphoribosyltransferase, hypoxanthine phosphoribosyl transferase, hypoxanthine phosphoribosyltransferase, hypoxanthine phosphoribosyltransferase 1, hypoxanthine PRTase, hypoxanthine-guanine phosphoribosyl transferase, hypoxanthine-guanine phosphoribosyltransferase, hypoxanthine-guanine phosphoribosyltransferase/adenylate kinase, hypoxanthine-guanine-(xanthine) phosphoribosyltransferase, hypoxanthine-guanine-xanthine phosphoribosyltransferase, hypoxanthine-guanine-[xanthine]-phosphoribosyltransferase, hypoxanthine-xanthine-guanine phosphoribosyltransferase, IMP pyrophosphorylase, IMP-GMP pyrophosphorylase, inosinate pyrophosphorylase, inosine 5'-phosphate pyrophosphorylase, inosine monophosphate:pyrophosphate phosphoribosyltransferase, inosinic acid pyrophosphorylase, inosinic pyrophosphorylase, More, MSMEG_6110, MtHGPRT, Pf HGXPRT, PF3D7_1012400, PfHGXPRT, PhHGXPRT, phosphoribosyltransferase, 6-mercaptopurine, phosphoribosyltransferase, hypoxanthine, PhPRT, plasma hypoxanthine-guanine phosphoribosyl transferase, PRT, purine-6-thiol phosphoribosyltransferase, PvHGPRT, SmHGPRT1, SSO2424, Tb927.10.1400, TcHPRT, transphosphoribosidase, TthHPRT, TT_C1766, ZgHGPRT/AMPK

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.8 hypoxanthine phosphoribosyltransferase

Purification

Purification on EC 2.4.2.8 - hypoxanthine phosphoribosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 methods
3 isoenzymes
-
from brain and liver
-
from erythrocytes and from brain
-
native enzyme by anion exchange and cation exchange chromatographies and affinity chromatography on immobilized Reactive Red 120, method development, overview, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
partial
-
recombinant chimeric mutant enzyme from Escherichia coli strain Su609 by anion exchange chromatography
recombinant enzyme
recombinant enzyme from Escherichia coli strain BL21 (DE3) 1-2fold to homogeneity by anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography
-
recombinant enzyme from Escherichia coli, 16fold to homogeneity by ion exchange and adsorption chromatography, and gel filtration
recombinant from Escherichia coli
recombinant from Escherichia coli , large scale
-
recombinant His-tagged enzyme from Escherichia coli strain Lemo21(DE3) by cobalt affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, recombinant nontagged enzyme from Escherichia coli by anion exchange chromatography, ammonium sulfate fractionation, dialysis, and gel filtration, both to over 95% purity
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity xhromatography
recombinant HPRT from Escherichia coli strain C3030/pER by hydrophobic interaction chromatography and ultrafiltration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli by GMP affinity and cation exchange chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain DH5alpha by GMP affinity chromatography
-
recombinant wild-type and mutant from Escherichia coli
-
recombinant wild-type and mutant L44F from Escherichia coli
-
recombinant wild-type and mutant L44F from Escherichia coli, the enzyme shows very low activity
-
recombinant wild-type and mutants F36L, F36G, F36K from Escherichia coli
recombinant wild-type enzyme and His-tagged mutant L160I enzyme from Escherichia coli, wild-type enzyme to homogeneity by ion exchange and adsorption chromatography, and gel filtration, mutant in a multistep procedure, overview
-
recombinant wild-type enzyme and loop II-deletion mutant from Escherichia coli
-
recombinant wild-type enzyme and mutant/s from Escherichia coli
-
recombinant wild-type enzyme and mutants from Escherichia coli
-
thermal treatment (1 h, 70°C), immobilized metal affinity chromatography with Hi-Trap nickel chelating column (pH 7.5, elution with 200 mM imidazole), cleavage of His-tag with thrombin followed by second IMAC
-