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0.0139 - 74.8
5-phospho-alpha-D-ribose 1-diphosphate
0.001 - 77
inosine monophosphate
additional information
additional information
-
0.0139
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 25°C, recombinant enzyme, with guanine
0.04
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.05
5-phospho-alpha-D-ribose 1-diphosphate
-
loop II-deletion mutant
0.1
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.1
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.1
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181F
0.1
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181Y
0.2
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.2
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, at pH 7.4 and 23°C
0.2
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.2
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.2
5-phospho-alpha-D-ribose 1-diphosphate
unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.2
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181F
0.2
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated wild-type enzyme
0.3
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.32
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant nonactivated mutant W181S
0.4
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.4
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.48
5-phospho-alpha-D-ribose 1-diphosphate
-
+/-0.02, 50°C
0.5
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181Y
0.66
5-phospho-alpha-D-ribose 1-diphosphate
-
+/-0.03, 40°C
0.67
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated mutant W181S
0.97
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 25°C, recombinant enzyme, with hypoxanthine
1
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C
1.6
5-phospho-alpha-D-ribose 1-diphosphate
activated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C
1.6
5-phospho-alpha-D-ribose 1-diphosphate
IMP-activated enzyme, at pH 7.4 and 23°C
1.6
5-phospho-alpha-D-ribose 1-diphosphate
pH 7.4, 37°C, recombinant IMP-activated wild-type enzyme
1.68
5-phospho-alpha-D-ribose 1-diphosphate
-
+/-0.06, 60°C
2.5
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type enzyme
2.8
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant E196R
3.05
5-phospho-alpha-D-ribose 1-diphosphate
-
+/-0.07, 70°C
4.26
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.4, 12 mM MgCl2
4.7
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant mutant L67M
6
5-phospho-alpha-D-ribose 1-diphosphate
recombinant enzyme, pH 8.0, 70°C
10.9
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant E196Q
14.2
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant E196D
20
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant mutant G69S
20.3
5-phospho-alpha-D-ribose 1-diphosphate
-
mutant E196A
23.2
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type enzyme
23.2
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant wild-type enzyme
23.2
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant enzyme, with hypoxanthine
32.2
5-phospho-alpha-D-ribose 1-diphosphate
-
pH 7.5, 37°C, recombinant enzyme, with guanine
36.3
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type enzyme
74.8
5-phospho-alpha-D-ribose 1-diphosphate
-
wild-type enzyme, forward reaction
0.002
diphosphate
-
loop II-deletion mutant
0.041
diphosphate
-
wild-type enzyme
0.1
diphosphate
-
pH 7.5, 37°C, recombinant enzyme, with GMP
0.12
diphosphate
-
pH 7.5, 37°C, recombinant mutant L67M
0.31
diphosphate
-
pH 7.5, 37°C, recombinant mutant G69S
0.33
diphosphate
-
mutant E196D
0.47
diphosphate
-
pH 7.5, 37°C, recombinant enzyme, with IMP
0.477
diphosphate
-
wild-type enzyme
0.48
diphosphate
-
pH 7.5, 37°C, recombinant wild-type enzyme
90
diphosphate
-
wild-type enzyme
0.09
GMP
-
pH 7.5, 37°C, recombinant enzyme
5.8
GMP
-
wild-type enzyme, reverse reaction
0.0024
guanine
pH 7.4, 25°C
0.0027
guanine
pH 7.4, 50°C
0.0033
guanine
pH 7.4, 37°C
0.013
guanine
-
purified recombinant chimeric enzyme DS1
0.013
guanine
-
purified recombinant chimeric enzyme DS1
0.039
guanine
-
loop II-deletion mutant
0.06
guanine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.1
guanine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.14
guanine
-
pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.193
guanine
at pH 7.5 and 25°C
0.21
guanine
-
+/-0.01, 50°C
0.3
guanine
activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.37
guanine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.37
guanine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.4
guanine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
1.1
guanine
-
pH 7.4, 22°C, recombinant mutant L44F
1.2
guanine
-
pH 7.4, 22°C, recombinant wild-type enzyme
1.2
guanine
pH 7.4, 28°C, wild-type enzyme
1.43
guanine
-
+/-0.02, 70°C
2
guanine
recombinant enzyme, pH 8.0, 70°C
3.4
guanine
pH 7.4, 22°C, recombinant mutant F36G
4.3
guanine
-
native enzyme
5
guanine
-
recombinant enzyme
5.7
guanine
-
wild-type enzyme
6.3
guanine
pH 7.4, 22°C, recombinant mutant F36K
10.5
guanine
-
wild-type enzyme
12.1
guanine
-
wild-type enzyme
15.8
guanine
-
mutant enzyme
18
guanine
pH 7.4, 22°C, recombinant mutant F36L
22.1
guanine
-
pH 7.5, 37°C, recombinant mutant G69S
23.8
guanine
recombinant wild-type enzyme, pH 7.4, 25°C
25.5
guanine
pH 7.4, 22°C, recombinant wild-type enzyme
25.5
guanine
pH 7.4, 28°C, wild-type enzyme
32.9
guanine
-
wild-type enzyme
32.9
guanine
-
pH 7.5, 37°C, recombinant wild-type enzyme
32.9
guanine
-
pH 7.5, 37°C, recombinant enzyme
41.3
guanine
-
wild-type enzyme
76.7
guanine
-
wild-type, forward reaction
0.06
hypoxanthine
-
loop II-deletion mutant
0.073
hypoxanthine
-
purified recombinant chimeric enzyme DS1
0.073
hypoxanthine
-
purified recombinant chimeric enzyme DS1
0.1
hypoxanthine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.2
hypoxanthine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.3
hypoxanthine
-
pH 7.5, 37°C, recombinant mutant L67M
0.3
hypoxanthine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.3
hypoxanthine
activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.38
hypoxanthine
-
+/-0.01, 40°C
0.47
hypoxanthine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.47
hypoxanthine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.83
hypoxanthine
-
+/-0.04, 50°C
0.89
hypoxanthine
at pH 7.5 and 25°C
0.9
hypoxanthine
-
pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate
0.94
hypoxanthine
-
pH 7.4, 22°C, recombinant mutant L44F
1.13
hypoxanthine
-
pH 7.4, 22°C, recombinant wild-type enzyme
1.13
hypoxanthine
pH 7.4, 28°C, wild-type enzyme
1.38
hypoxanthine
-
+/-0.03, 60°C
2.4
hypoxanthine
-
native enzyme
2.6
hypoxanthine
-
wild-type enzyme
2.6
hypoxanthine
-
recombinant enzyme
3.1
hypoxanthine
pH 7.4, 22°C, recombinant mutant F36G
3.16
hypoxanthine
-
+/-0.08, 70°C
3.4
hypoxanthine
pH 7.4, 22°C, recombinant mutant F36K
3.8
hypoxanthine
-
mutant E196R
4.12
hypoxanthine
pH 7.4, 25°C
5.4
hypoxanthine
pH 7.4, 22°C, recombinant mutant F36L
5.7
hypoxanthine
-
wild-type enzyme
5.7
hypoxanthine
-
wild-type enzyme
6.2
hypoxanthine
-
mutant enzyme
6.71
hypoxanthine
-
mutant T47K
7.1
hypoxanthine
pH 7.4, 22°C, recombinant wild-type enzyme
7.1
hypoxanthine
pH 7.4, 28°C, wild-type enzyme
7.97
hypoxanthine
pH 7.4, 37°C
8.5 - 9.3
hypoxanthine
-
recombinant enzyme
8.54
hypoxanthine
-
wild-type enzyme
9
hypoxanthine
recombinant enzyme, pH 8.0, 70°C
9.1
hypoxanthine
pH 7.9, 25°C
13.7
hypoxanthine
-
mutant E196Q
13.8
hypoxanthine
-
mutant E196D
15.9
hypoxanthine
pH 7.4, 50°C
17.1
hypoxanthine
recombinant wild-type enzyme, pH 7.4, 25°C
20.3
hypoxanthine
-
mutant E196A
22
hypoxanthine
-
pH 7.5, 37°C, recombinant mutant G69S
22.9
hypoxanthine
-
wild-type enzyme
22.9
hypoxanthine
-
pH 7.5, 37°C, recombinant wild-type enzyme
22.9
hypoxanthine
-
pH 7.5, 37°C, recombinant enzyme
0.0036
IMP
unactivated enzyme, at pH 7.4 and 23°C
0.0042
IMP
IMP-activated enzyme, at pH 7.4 and 23°C
0.038
IMP
-
wild-type enzyme
0.07
IMP
-
pH 7.5, 37°C, recombinant mutant L67M
0.19
IMP
-
pH 7.5, 37°C, recombinant mutant G69S
0.46
IMP
-
pH 7.5, 37°C, recombinant enzyme
0.47
IMP
-
pH 7.5, 37°C, recombinant wild-type enzyme
0.001
inosine monophosphate
-
loop II-deletion mutant
0.23 - 0.3
inosine monophosphate
-
recombinant enzyme, reverse reaction
0.472
inosine monophosphate
-
wild-type enzyme
77
inosine monophosphate
-
wild-type enzyme
0.062
xanthine
-
purified recombinant chimeric enzyme DS1
0.062
xanthine
-
purified recombinant chimeric enzyme DS1
0.07
xanthine
pH 7.4, 22°C, recombinant mutant F36L
0.08
xanthine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.08
xanthine
pH 7.4, 28°C, recombinant chimeric mutant enzyme
0.2
xanthine
unactivated enzyme, at pH 7.4 and 23°C
0.2
xanthine
unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
0.3
xanthine
unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
0.68
xanthine
-
+/-0.04, 50°C
0.8
xanthine
activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C
1
xanthine
activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C
1.2
xanthine
-
pH 7.4, 22°C, recombinant mutant L44F
1.6
xanthine
IMP-activated enzyme, at pH 7.4 and 23°C
2.2
xanthine
-
+/-0.08, 70°C
2.9
xanthine
-
pH 7.4, 22°C, recombinant wild-type enzyme
2.9
xanthine
pH 7.4, 28°C, wild-type enzyme
additional information
additional information
-
kinetics
-
additional information
additional information
-
kcat of mutant enzymes
-
additional information
additional information
-
wild-type and mutants: kcat for substrates hypoxanthine, guanine, xanthine, IMP, GMP, XMP, diphosphate, 5-phosphoribosyl 1-diphosphate
-
additional information
additional information
-
wild-type and diverse mutant enzymes, overview
-
additional information
additional information
-
increasing kcat with temperature (40-70°C) for all substrates tested
-
additional information
additional information
-
kcat/KM for 5-phospho-alpha-D-ribose 1-diphosphate = 0.00093 mM(-1)*min(-1) at 40°C, 0.00069 mM(-1)*min(-1) at 50°C, 0.00357 mM(-1)*min(-1) at 60°C, 0.00496 mM(-1)*min(-1) at 70°C
-
additional information
additional information
-
kcat/KM for guanine = 0.00262 mM(-1)*min(-1) at 50°C, 0.02631 mM(-1)*min(-1) at 70°C
-
additional information
additional information
-
kcat/KM for hypoxanthine = 0.00579 mM(-1)*min(-1) at 40°C, 0.00708 mM(-1)*min(-1) at 50°C, 0.01719 mM(-1)*min(-1) at 60°C, 0.03107 mM(-1)*min(-1) at 70°C
-
additional information
additional information
-
kcat/KM for hypoxanthine similar to kcat/KM for guanine, kcat/KM for xanthine approximately 20 times lower
-
additional information
additional information
-
kcat/KM for xanthine = 0.00040 mM(-1)*min(-1) at 50°C, 0.00139 mM(-1)*min(-1) at 70°C
-