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Literature summary for 2.4.2.8 extracted from
- Purification of human malaria parasite hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) using immobilized Reactive Red 120 (2007), Protein Expr. Purif., 52, 153-158.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Plasmodium falciparum |  |
| additional information | no inhibition by EGTA | Plasmodium falciparum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Plasmodium falciparum | |
| additional information | no activation by Ca2+ | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | the enzyme is involved in the purine salvage pathway | IMP + diphosphate | - |
? | |
| additional information | Plasmodium falciparum | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange and cation exchange chromatographies and affinity chromatography on immobilized Reactive Red 120, method development, overview, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0000021 | 0.0000072 | purified native enzyme | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme is involved in the purine salvage pathway | Plasmodium falciparum | IMP + diphosphate | - |
? | |
| additional information | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | Plasmodium falciparum | ? | - |
? | |
| xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme also performs the reaction of xanthine phosphoribosyltransferase, EC 2.4.2.22 | Plasmodium falciparum | XMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGXPRT | - |
Plasmodium falciparum |
| hypoxanthine guanine xanthine phosphoribosyltransferase | - |
Plasmodium falciparum |
| More | cf. EC 2.4.2.22 | Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at, substrates hypoxanthine and guanine | Plasmodium falciparum |
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