Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Plasmodium falciparum | |
additional information | no inhibition by EGTA | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Plasmodium falciparum | |
additional information | no activation by Ca2+ | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | the enzyme is involved in the purine salvage pathway | IMP + diphosphate | - |
? | |
additional information | Plasmodium falciparum | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by anion exchange and cation exchange chromatographies and affinity chromatography on immobilized Reactive Red 120, method development, overview, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000021 | 0.0000072 | purified native enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme is involved in the purine salvage pathway | Plasmodium falciparum | IMP + diphosphate | - |
? | |
additional information | the parasite depends on the human host purine salvage pathway enzyme hypoxanthine guanine xanthine phosphoribosyltransferase for survival, because it does not have a de novo pathway for synthesis of nucleotides on its own | Plasmodium falciparum | ? | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | the enzyme also performs the reaction of xanthine phosphoribosyltransferase, EC 2.4.2.22 | Plasmodium falciparum | XMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HGXPRT | - |
Plasmodium falciparum |
hypoxanthine guanine xanthine phosphoribosyltransferase | - |
Plasmodium falciparum |
More | cf. EC 2.4.2.22 | Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at, substrates hypoxanthine and guanine | Plasmodium falciparum |