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Literature summary for 2.4.2.8 extracted from

  • Nagy, M.; Ribet, A.M.
    Purification and comparative study of adenine and guanine phosphoribosyltransferases from Schizosaccharomyces pombe (1977), Eur. J. Biochem., 77, 77-85.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Schizosaccharomyces pombe
CDP
-
Schizosaccharomyces pombe
diphosphate
-
Schizosaccharomyces pombe
GDP
-
Schizosaccharomyces pombe
GMP
-
Schizosaccharomyces pombe
GTP
-
Schizosaccharomyces pombe
IDP
-
Schizosaccharomyces pombe
IMP
-
Schizosaccharomyces pombe
ITP weak Schizosaccharomyces pombe
Mg2+ inhibitory effects are noncompetitive against 5-phosphoribose 1-diphosphate Schizosaccharomyces pombe
additional information mechanism of product inhibition; no inhibition by ADP, ATP, dAMP, UMP, and UTP Schizosaccharomyces pombe
UDP weak Schizosaccharomyces pombe
XMP
-
Schizosaccharomyces pombe

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
guanine
-
Schizosaccharomyces pombe
0.1
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement Schizosaccharomyces pombe
Mg2+ maximal activity at: 0.5-1 mM MgCl2 Schizosaccharomyces pombe

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
gel filtration in presence of MgCl2 Schizosaccharomyces pombe
48000
-
gel filtration in absence of MgCl2 Schizosaccharomyces pombe

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.75
-
-
Schizosaccharomyces pombe

Storage Stability

Storage Stability Organism
-20°C, purified enzyme, 6 mM 5-phospho-alpha-D-ribose 1-diphosphate, 2 mM MgCl2, stable for at least a month Schizosaccharomyces pombe

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schizosaccharomyces pombe IMP + diphosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1.5 mM GMP, 10 min, complete loss of activity Schizosaccharomyces pombe

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8 2 zones of pH-optima: pH 7.6-8.0 and pH 9.2-9.5 Schizosaccharomyces pombe
9.2 9.5 2 zones of pH-optima: pH 7.6-8.0 and pH 9.2-9.5 Schizosaccharomyces pombe