Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Artemia sp. | |
Ca2+ | strong | Artemia sp. | |
GMP | mixed inhibition | Artemia sp. | |
guanine | substrate inhibition | Artemia sp. | |
hypoxanthine | substrate inhibition | Artemia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
guanine | below | Artemia sp. | |
0.001 | - |
hypoxanthine | below | Artemia sp. | |
0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Artemia sp. | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Artemia sp. | |
Mg2+ | maximal activity at: 1 mM | Artemia sp. | |
Mg2+ | Km: 0.04-0.05 mM | Artemia sp. | |
Mn2+ | can replace Mg2+ | Artemia sp. | |
Mn2+ | Km: 0.015-0.020 mM | Artemia sp. | |
Zn2+ | activates, can replace Mg2+, Km: 0.066-0.075 mM | Artemia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
4 * 19000, SDS-PAGE | Artemia sp. |
66000 | - |
gel filtration | Artemia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemia sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Artemia sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cyst | - |
Artemia sp. | - |
nauplius | - |
Artemia sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.65 | - |
purified enzyme | Artemia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Artemia sp. | IMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 19000, SDS-PAGE | Artemia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Artemia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
2 buffer-independent pH-optima: pH 7.0 and pH 9.5 | Artemia sp. |
9.5 | - |
2 buffer-independent pH-optima: pH 7.0 and pH 9.5 | Artemia sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
guanine | - |
Artemia sp. | |
0.08 | - |
hypoxanthine | - |
Artemia sp. |