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26.4
D-galactose
in McIlvaine buffer (pH 7.5), at 35°C
2 - 37
D-tagatose
in McIlvaine buffer (pH 7.5), at 35°C
0.23 - 12.9
UDP-alpha-D-galactose
0.06 - 260
UDP-alpha-D-glucose
0.057 - 0.29
UDP-D-galactose
0.31
UDP-D-glucose
50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme
0.15
UDP-D-xylose
pH 8.6, 25°C
0.035 - 3.9
UDP-galactose
0.16
UDP-L-arabinose
pH 8.6, 25°C
1.06
UDP-N-acetyl-alpha-D-galactosamine
pH 7.0, 80°C
0.146 - 2.4
UDP-N-acetyl-alpha-D-glucosamine
0.15
UDP-xylose
pH 8.6, 25°C
0.0083 - 1.67
UDPgalactose
additional information
additional information
-
0.23
UDP-alpha-D-galactose
pH 6.5, 60°C
5.53
UDP-alpha-D-galactose
pH 7.0, 80°C
12.9
UDP-alpha-D-galactose
recombinant enzyme, pH 7.0, 80°C
0.06
UDP-alpha-D-glucose
mutant L320Y, pH 8.5, 25°C
0.1 - 1
UDP-alpha-D-glucose
wild-type enzyme, pH 8.5, 25°C
2 - 6
UDP-alpha-D-glucose
-
pH 6.2, temperature not specified in the publication, recombinant mutant Y149F
2.24
UDP-alpha-D-glucose
pH 6.5, 60°C
5.53
UDP-alpha-D-glucose
recombinant enzyme, pH 7.0, 80°C
12.9
UDP-alpha-D-glucose
pH 7.0, 80°C
83
UDP-alpha-D-glucose
-
pH 6.2, temperature not specified in the publication, recombinant mutant K153M
110
UDP-alpha-D-glucose
-
pH 6.2, temperature not specified in the publication, recombinant mutant S124A
230
UDP-alpha-D-glucose
-
pH 6.2, temperature not specified in the publication, recombinant wild-type enzyme
260
UDP-alpha-D-glucose
-
pH 6.2, temperature not specified in the publication, recombinant S124T
0.057
UDP-D-galactose
recombinant enzyme UGE4, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase
0.068
UDP-D-galactose
recombinant enzyme UGE3, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase
0.087
UDP-D-galactose
recombinant Arabidopsis thaliana enzyme UGE1, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-Glc dehydrogenase
0.095
UDP-D-galactose
recombinant Arabidopsis thaliana enzyme UGE2, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDPGlc dehydrogenase
0.15
UDP-D-galactose
recombinant enzyme UGE5, 100 mM glycine/NaOH (pH 8.6), 0.015-1 mM UDP-Gal, 2 mM NAD+ and 40 mU of UDP-glucose dehydrogenase
0.29
UDP-D-galactose
50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme
0.04
UDP-Gal
-
0.035
UDP-galactose
-
37°C, pH 8.8, mutant enzyme L313M
0.04
UDP-galactose
pH 8.0, 25°C
0.048
UDP-galactose
-
wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C
0.066
UDP-galactose
-
37°C, pH 8.8, mutant enzyme K257R
0.069
UDP-galactose
-
37°C, pH 8.8, wild-type enzyme
0.078
UDP-galactose
-
37°C, pH 8.8, mutant enzyme G319E
0.082
UDP-galactose
-
37°C, pH 8.8, mutant enzyme N34S
0.093
UDP-galactose
-
37°C, pH 8.8, mutant enzyme G90E
0.097
UDP-galactose
-
37°C, pH 8.8, mutant enzyme L183P
0.099
UDP-galactose
-
37°C, pH 8.8, mutant enzyme R335H
0.1 - 0.13
UDP-galactose
-
Different enzyme concentrations give slight variations in activity.
0.11
UDP-galactose
-
25°C, pH 8.8
0.14
UDP-galactose
-
37°C, pH 8.8, mutant enzyme D103G
0.16
UDP-galactose
-
24°C, pH 7.0
0.16
UDP-galactose
-
37°C, pH 8.8, mutant enzyme V94M
0.17 - 0.22
UDP-galactose
-
Different enzyme concentrations give slight variations in activity.
0.19 - 0.22
UDP-galactose
-
Different enzyme concentrations give slight variations in activity.
0.2
UDP-galactose
-
mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C
0.23 - 0.3
UDP-galactose
-
Different enzyme concentrations give slight variations in activity.
0.25
UDP-galactose
-
mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C
0.255
UDP-galactose
in McIlvaine buffer (pH 7.5), at 35°C
0.258
UDP-galactose
-
pH 8.9, 50°C, recombinant GalE
0.258
UDP-galactose
-
recombinant enzyme, 100 mM glycine-NaOH (pH 8.9), 4 mM UDP-galactose, 1 mM beta-NAD+, 8.3 mM MgCl2, and 5.4 U of UDP-glucose dehydrogenase
0.29
UDP-galactose
pH 8.6, 25°C
0.38
UDP-galactose
-
wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C
0.5
UDP-galactose
-
wild type recombinant enzyme, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C
0.784
UDP-galactose
-
37°C
0.8
UDP-galactose
-
mutant enzyme K153N, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C
1
UDP-galactose
-
mutant enzyme Y149G, in 20 mM sodium phosphate buffer, pH 8.0, at 25°C
3.9
UDP-galactose
in 100 mM MES-NaOH (pH 5.5), at 70°C
0.16
UDP-GalNAc
-
0.16
UDP-GalNAc
pH 8.0, 25°C
0.055
UDP-Glc
-
0.2
UDP-GlcNAc
-
0.2
UDP-GlcNAc
pH 8.0, 25°C
0.055
UDP-glucose
pH 8.0, 25°C
0.09
UDP-glucose
-
values are indirectly determined from the Haldane relationship
0.13
UDP-glucose
-
values are indirectly determined from the Haldane relationship
0.19
UDP-glucose
-
values are indirectly determined from the Haldane relationship
0.31
UDP-glucose
pH 8.6, 25°C
0.56
UDP-glucose
-
values are indirectly determined from the Haldane relationship
0.76
UDP-glucose
-
values are indirectly determined from the Haldane relationship
1.2
UDP-glucose
-
24°C, pH 7.0
0.146
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, mutant G118S/G119S
0.243
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, mutant G118A/G119A
0.316
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, mutant S279Y
0.362
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, wild-type enzyme
0.426
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, mutant T117S
0.437
UDP-N-acetyl-alpha-D-glucosamine
-
pH 7.5, 45°C, mutant S116A
2.4
UDP-N-acetyl-alpha-D-glucosamine
pH 7.0, 80°C
0.0083
UDPgalactose
-
mammary enzyme
0.017
UDPgalactose
-
liver enzyme
0.026
UDPgalactose
-
mutant Y149F
0.048
UDPgalactose
-
mutant S124A
0.1
UDPgalactose
-
native and renatured enzyme
0.12
UDPgalactose
Saccharomyces fragilis
-
-
0.14
UDPgalactose
-
fusion enzyme consisting of UDP-galactose 4-epimerase and galactose-1-phosphate uridylyltransferase with an intervening Ala3 linker
0.18
UDPgalactose
-
native and renatured enzyme
0.225
UDPgalactose
-
wild type enzyme
0.256
UDPgalactose
-
mutant S124T
1.2
UDPgalactose
Torulopsis candida
-
-
0.25
UDPglucose
-
-
additional information
additional information
Saccharomyces fragilis
-
classical hyperbolic kinetics with UDPgalactose, allosteric kinetics with UDPglucose
-
additional information
additional information
Saccharomyces fragilis
-
enzyme shows Michaelis kinetics with UDPgalactose as the substrate and allosteric kinetics with UDPglucose as the substrate
-
additional information
additional information
kinetic analysis
-
additional information
additional information
-
kinetic analysis
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
-
biphasic Michaelis-Menten kinetics, kinetic patterns, overview. Michaelis-Menten relationship of the monomeric epimerase shows hyperbolic dependency
-
additional information
additional information
kinetic analyis of wild-type and mutant enzymes, overview
-
additional information
additional information
-
kinetic analyis of wild-type and mutant enzymes, overview
-