Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-UMP | - |
Bos taurus | |
5'-UMP | the native enzyme is completely insensitive to inhibition, the desensitized enzyme is strongly inhibited. Desensitization by heat converts the enzyme to its ultimate catalytic form | Escherichia coli | |
5'-UMP | - |
Saccharomyces fragilis | |
5'-UMP | - |
Torulopsis candida | |
5-(adenosine-5'-diphosphoryl)-D-ribose | reductive inhibition | Escherichia coli | |
5-(thymidine-5'-diphosphoryl)-D-glucose | reductive inhibition | Escherichia coli | |
5-(Thymidine-5'-diphosphoryl)-D-ribose | reductive inhibition | Escherichia coli | |
D-galactose | - |
Torulopsis candida | |
glucose plus UMP | - |
Escherichia coli | |
glucose plus UMP | - |
Torulopsis candida | |
NaBH4 | reductive inhibition | Escherichia coli | |
NADH | - |
Bos taurus | |
NADH | - |
Escherichia coli | |
Thymidine diphospho-6-deoxy-D-xylo-4-hexosulose | reductive inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
UDPgalactose | - |
Escherichia coli | |
1.2 | - |
UDPgalactose | - |
Torulopsis candida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Escherichia coli |
60000 | - |
2 * 60000, enzyme exists in an active dimeric and an active tetrameric form | Kluyveromyces marxianus |
60000 | - |
4 * 60000, enzyme exists in an active dimeric and an active tetrameric form | Kluyveromyces marxianus |
80000 | - |
sedimentation equilibrium studies | Escherichia coli |
120000 | - |
sedimentation equilibrium measurement, addition of NAD+, active dimeric enzyme form | Kluyveromyces marxianus |
240000 | - |
sedimentation equilibrium measurement, addition of NAD+, 0.01 M Tris buffer with cations, active tetrameric form | Kluyveromyces marxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Escherichia coli | - |
- |
- |
Kluyveromyces marxianus | - |
- |
- |
Saccharomyces fragilis | - |
- |
- |
Torulopsis candida | - |
- |
- |
Vigna radiata var. radiata | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glucose | - |
Escherichia coli | ? | - |
? | |
CDP-D-glucose | - |
Escherichia coli | ? | - |
? | |
Deoxy-UDP-D-glucose | - |
Escherichia coli | ? | - |
? | |
TDP-6-deoxy-D-galactose | - |
Escherichia coli | ? | - |
? | |
TDP-D-glucose | - |
Escherichia coli | TDP-D-galactose | - |
? | |
UDP-6-deoxy-D-glucose | - |
Escherichia coli | ? | - |
? | |
UDP-D-fucose | - |
Escherichia coli | ? | - |
? | |
UDP-D-galactose-hexodialose | - |
Escherichia coli | ? | - |
? | |
UDP-D-xylose | - |
Escherichia coli | ? | - |
? | |
UDP-galactose | - |
Vigna radiata var. radiata | UDP-glucose | - |
? | |
UDP-galactose | - |
Escherichia coli | UDP-glucose | - |
? | |
UDP-galactose | - |
Bos taurus | UDP-glucose | - |
? | |
UDP-galactose | - |
Saccharomyces fragilis | UDP-glucose | - |
? | |
UDP-galactose | - |
Kluyveromyces marxianus | UDP-glucose | - |
? | |
UDP-galactose | - |
Torulopsis candida | UDP-glucose | - |
? | |
UDP-L-arabinose | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | Escherichia coli |
dimer | 2 * 60000, enzyme exists in an active dimeric and an active tetrameric form | Kluyveromyces marxianus |
tetramer | - |
Escherichia coli |
tetramer | 4 * 60000, enzyme exists in an active dimeric and an active tetrameric form | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NAD+ | - |
Bos taurus | |
NAD+ | depends on addition of NAD+ for catalytic acitivity, Km: 0.14 mM. Cannot be replaced by NADP+ | Torulopsis candida |