General Stability | Organism |
---|---|
PCMB dissociates the native epimerase into inactive mercurated monomers, reconstitution of the functional holoenzyme is done by reduction with dithiothreitol and addition of extra NAD+, reactivation is most effective at pH 8.1 | Kluyveromyces marxianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-UMP | strong, competitive | Kluyveromyces marxianus | |
L-arabinose | on treatment with L-arabinose, 2 mM, in presence of UMP, 0.5 mM, both the native enzyme and the reconstituted enzyme are inactivated at an indistinguishable rate of inactivation | Kluyveromyces marxianus | |
PCMB | dissociates the native epimerase into inactive mercurated monomers, reconstitution of the functional holoenzyme is done by reduction with dithiothreitol and addition of extra NAD+, reactivation is most effective at pH 8.1 | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
UDP-galactose | 25°C, pH 8.8 | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000 | Kluyveromyces marxianus |
158000 | - |
gel filtration | Kluyveromyces marxianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose | - |
Kluyveromyces marxianus | UDP-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000 | Kluyveromyces marxianus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | one mol firmly bound per dimer | Kluyveromyces marxianus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
5'-UMP | 25°C, pH 8.8 | Kluyveromyces marxianus |