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Literature summary for 5.1.3.2 extracted from

  • Majumdar, S.; Bhattacharjee, H.; Bhattacharyya, D.; Bhaduri, A.
    UDP-galactose 4-epimerase from Kluyveromyces fragilis: reconstitution of holoenzyme structure after dissociation with parachloromercuribenzoate (1998), Eur. J. Biochem., 257, 427-433.
    View publication on PubMed

General Stability

General Stability Organism
PCMB dissociates the native epimerase into inactive mercurated monomers, reconstitution of the functional holoenzyme is done by reduction with dithiothreitol and addition of extra NAD+, reactivation is most effective at pH 8.1 Kluyveromyces marxianus

Inhibitors

Inhibitors Comment Organism Structure
5'-UMP strong, competitive Kluyveromyces marxianus
L-arabinose on treatment with L-arabinose, 2 mM, in presence of UMP, 0.5 mM, both the native enzyme and the reconstituted enzyme are inactivated at an indistinguishable rate of inactivation Kluyveromyces marxianus
PCMB dissociates the native epimerase into inactive mercurated monomers, reconstitution of the functional holoenzyme is done by reduction with dithiothreitol and addition of extra NAD+, reactivation is most effective at pH 8.1 Kluyveromyces marxianus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
UDP-galactose 25°C, pH 8.8 Kluyveromyces marxianus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
2 * 75000 Kluyveromyces marxianus
158000
-
gel filtration Kluyveromyces marxianus

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose
-
Kluyveromyces marxianus UDP-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 75000 Kluyveromyces marxianus

Cofactor

Cofactor Comment Organism Structure
NAD+ one mol firmly bound per dimer Kluyveromyces marxianus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
5'-UMP 25°C, pH 8.8 Kluyveromyces marxianus