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Literature summary for 5.1.3.2 extracted from
- Crystal structure of UDP-galactose 4-epimerase from the hyperthermophilic archaeon Pyrobaculum calidifontis (2011), Arch. Biochem. Biophys., 51, 126-134.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pyrobaculum calidifontis |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapor diffusion method, using 29% (v/v) 2-ethoxyethanol and 0.1 M cacodylate buffer (pH 6.6), at 20°C | Pyrobaculum calidifontis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a GalE loop deletion mutant (mut1), in which residues 32-43 (NLSSGRREFVNP) of the NAD-binding loop are replaced with residues 33-40 (IVQRDTGG) of the corresponding loop from Thermoplasma volcanium binds NAD+ in a loose, reversible manner | Pyrobaculum calidifontis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.9 | - |
UDP-galactose | in 100 mM MES-NaOH (pH 5.5), at 70°C | Pyrobaculum calidifontis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
2 * 32000, SDS-PAGE | Pyrobaculum calidifontis |
| 51000 | - |
gel filtration | Pyrobaculum calidifontis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | Pyrobaculum calidifontis | - |
UDP-alpha-D-galactose | - |
? | |
| UDP-galactose | Pyrobaculum calidifontis | - |
UDP-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum calidifontis | A3MUJ4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Toyopearl column chromatography and Superdex 200 gel filtration | Pyrobaculum calidifontis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | - |
Pyrobaculum calidifontis | UDP-alpha-D-galactose | - |
? | |
| UDP-galactose | - |
Pyrobaculum calidifontis | UDP-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 32000, SDS-PAGE | Pyrobaculum calidifontis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GalE | - |
Pyrobaculum calidifontis |
| UDP-galactose-4-epimerase | - |
Pyrobaculum calidifontis |
| UGE | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Pyrobaculum calidifontis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.84 | - |
UDP-galactose | in 100 mM MES-NaOH (pH 5.5), at 70°C | Pyrobaculum calidifontis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Pyrobaculum calidifontis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pyrobaculum calidifontis | |
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Release 2023.1 (January 2023)
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