Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Saccharomyces cerevisiae | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibited by combination of 100 microM NADH and 10 microM NAD+ | Arabidopsis thaliana | |
NaCl | - |
Arabidopsis thaliana | |
NADH | slightly inhibiting | Arabidopsis thaliana | |
NADPH | very weak inhibitory effect | Arabidopsis thaliana | |
UDP | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
UDP-glucose | values are indirectly determined from the Haldane relationship | Arabidopsis thaliana | |
0.1 | 0.13 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
0.13 | - |
UDP-glucose | values are indirectly determined from the Haldane relationship | Arabidopsis thaliana | |
0.17 | 0.22 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
0.19 | - |
UDP-glucose | values are indirectly determined from the Haldane relationship | Arabidopsis thaliana | |
0.19 | 0.22 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
0.23 | 0.3 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
0.56 | - |
UDP-glucose | values are indirectly determined from the Haldane relationship | Arabidopsis thaliana | |
0.76 | - |
UDP-glucose | values are indirectly determined from the Haldane relationship | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
Golgi apparatus | - |
Arabidopsis thaliana | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | increases the activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Arabidopsis thaliana |
40200 | - |
gel filtration | Arabidopsis thaliana |
40300 | - |
gel filtration | Arabidopsis thaliana |
40800 | - |
gel filtration | Arabidopsis thaliana |
41100 | - |
gel filtration | Arabidopsis thaliana |
84000 | - |
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model. | Arabidopsis thaliana |
91000 | - |
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model. | Arabidopsis thaliana |
98000 | - |
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model. | Arabidopsis thaliana |
103000 | - |
Dynamic Light Scattering, calculated from the hydrodynamic radius using the default volume shape hydration model. | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose | Arabidopsis thaliana | carbohydrate biosynthesis | UDP-D-galactose | - |
r | |
UDP-D-glucose | Arabidopsis thaliana | carbohydrate catabolism | UDP-D-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose | carbohydrate biosynthesis | Arabidopsis thaliana | UDP-D-galactose | - |
r | |
UDP-D-glucose | carbohydrate catabolism | Arabidopsis thaliana | UDP-D-galactose | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
UDP-D-glucose/UDP-D-galactose 4-epimerase | - |
Arabidopsis thaliana |
UGE1 | - |
Arabidopsis thaliana |
UGE2 | - |
Arabidopsis thaliana |
UGE3 | - |
Arabidopsis thaliana |
UGE4 | - |
Arabidopsis thaliana |
UGE5 | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Arabidopsis thaliana |
35 | - |
- |
Arabidopsis thaliana |
40 | - |
- |
Arabidopsis thaliana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | UDP-glucose | The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM). | Arabidopsis thaliana | |
19 | - |
UDP-glucose | The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM). | Arabidopsis thaliana | |
23 | 24 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
24 | - |
UDP-glucose | The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM). | Arabidopsis thaliana | |
28 | 34 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
32 | - |
UDP-glucose | The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM). | Arabidopsis thaliana | |
33 | - |
UDP-glucose | The approximate value is estimated from reaction velocities at saturating substrate concentrations (59 mM). | Arabidopsis thaliana | |
42 | 66 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
89 | 101 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana | |
115 | 128 | UDP-galactose | Different enzyme concentrations give slight variations in activity. | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | MES buffer | Arabidopsis thaliana |
6.5 | 9.5 | Tris/HCl buffer | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NAD+ | is particularly important | Arabidopsis thaliana |