Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells and in a gal10-null strain of Saccharomyces cerevisiae strain JFy3835 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D103G | the mutant demonstrates 82.1% residual activity | Homo sapiens |
G90E | the mutant demonstrates 1% residual activity | Homo sapiens |
L183P | the mutant demonstrates 3.3% residual activity | Homo sapiens |
M284K | the mutant is active in vivo, but not in vitro and shows reduced enzymatic activity (1.1% residual activity) and reduced stability towards denaturants in vitro | Homo sapiens |
N268D | the mutant demonstrates 63% residual activity | Homo sapiens |
N34S | the mutant demonstrates above 65.1% residual activity | Homo sapiens |
V94M | the mutant demonstrates 2.6% residual activity | Homo sapiens |
Y105C | the mutant demonstrates 13% residual activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
UDP-galactose | wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.2 | - |
UDP-galactose | mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C | Homo sapiens | |
0.25 | - |
UDP-galactose | mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.38 | - |
UDP-galactose | wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel agarose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose | - |
Homo sapiens | UDP-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GalE | - |
Homo sapiens |
UDP-galactose 4'-epimerase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |