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Literature summary for 5.1.3.2 extracted from

  • McCorvie, T.J.; Wasilenko, J.; Liu, Y.; Fridovich-Keil, J.L.; Timson, D.J.
    In vivo and in vitro function of human UDP-galactose 4-epimerase variants (2011), Biochimie, 93, 1747-1754.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta cells and in a gal10-null strain of Saccharomyces cerevisiae strain JFy3835 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D103G the mutant demonstrates 82.1% residual activity Homo sapiens
G90E the mutant demonstrates 1% residual activity Homo sapiens
L183P the mutant demonstrates 3.3% residual activity Homo sapiens
M284K the mutant is active in vivo, but not in vitro and shows reduced enzymatic activity (1.1% residual activity) and reduced stability towards denaturants in vitro Homo sapiens
N268D the mutant demonstrates 63% residual activity Homo sapiens
N34S the mutant demonstrates above 65.1% residual activity Homo sapiens
V94M the mutant demonstrates 2.6% residual activity Homo sapiens
Y105C the mutant demonstrates 13% residual activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 UDP-galactose wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.2
-
 UDP-galactose mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C Homo sapiens
0.25
-
 UDP-galactose mutant enzyme M284K, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 37°C Homo sapiens
0.38
-
 UDP-galactose wild type enzyme, in 20 mM HEPES-KOH, pH 7.5, 1 mM dithiohreitol, and 0.3 mg/ml bovine serum albumin, at 24°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel agarose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose
-
 Homo sapiens UDP-glucose
-
 r

Synonyms

Synonyms Comment Organism
GalE
-
 Homo sapiens
UDP-galactose 4'-epimerase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens