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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
+
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Activating Compound

Activating Compound on EC 4.2.2.10 - pectin lyase

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
10 mM, 124% of initial activity
alpha-expansin
-
alpha-expansin synergistically enhances the degradation activity of recombinant pectinase Pel1
-
ammonium sulfate
-
1 mM, 203% of initial activity
ascorbate
-
up to 3fold activation
azide
-
1 mM, 125% of initial activity
cAMP
-
enzyme induction at 5-10 mM
Co2+
-
25% increase of activity at 1 mM
EDTA
-
1 mM, 123% of initial activity
iodoacetate
-
10 mM, stimulates
L-arginine
-
up to 1.2fold activation
L-cystine
-
5 mM, 142% of initial activity
L-tryptophan
-
up to 1.3fold activation
mitomycin C
-
temperature-dependent induction of enzyme production, optimal at 30°C
Sodium arsenate
yeast extract components
-
induction of enzyme production by at least one organic component of yeast extract from sucrose containing culture medium, component is soluble in the nucleotide fraction, compound is thermostable, polar, dialyzable at 2000 Da, hydrolyzable by HCl, activated by boiling for up to 60 min
-
additional information
-