Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% increase of activity at 1 mM | Aspergillus flavus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Aspergillus flavus | |
Ca2+ | 88.3% residual activity at 1 mM | Aspergillus flavus | |
Cu2+ | complete inhibition at 1 mM | Aspergillus flavus | |
EDTA | 54.2% residual activity at 1 mM | Aspergillus flavus | |
Hg2+ | complete inhibition at 1 mM | Aspergillus flavus | |
K+ | 83.3% residual activity at 1 mM | Aspergillus flavus | |
Mg2+ | 87.5% residual activity at 1 mM | Aspergillus flavus | |
additional information | not influenced by Mn2+ | Aspergillus flavus | |
Na+ | 91.6% residual activity at 1 mM | Aspergillus flavus | |
potassium ferrocyanide | complete inhibition at 1 mM | Aspergillus flavus | |
potassium permanganate | complete inhibition at 1 mM | Aspergillus flavus | |
Sodium arsenate | 91.7% residual activity at 1 mM | Aspergillus flavus | |
Sodium azide | 75.0% residual activity at 1 mM | Aspergillus flavus | |
Zn2+ | 33.3% residual activity at 1 mM | Aspergillus flavus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
SDS-PAGE | Aspergillus flavus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus flavus | - |
- |
- |
Aspergillus flavus MTCC 7589 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, DEAE cellulose chromatography and Sephadex G-100 gel filtration | Aspergillus flavus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.316 | - |
crude enzyme, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C | Aspergillus flavus |
18.33 | - |
after 58.01fold purification, in 100 mM sodium phosphate buffer (pH 8.0), at 37°C | Aspergillus flavus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the purified enzyme shows efficacy in retting of Crotalaria juncea fibers | Aspergillus flavus | ? | - |
? | |
additional information | the purified enzyme shows efficacy in retting of Crotalaria juncea fibers | Aspergillus flavus MTCC 7589 | ? | - |
? | |
pectin | - |
Aspergillus flavus | 4,5-unsaturated oligogalacturonates | - |
? | |
pectin | - |
Aspergillus flavus MTCC 7589 | 4,5-unsaturated oligogalacturonates | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNL | - |
Aspergillus flavus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus flavus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme does not loose activity up to 50°C if exposed for 1 h, addition of ammonium sulfate in the range of 0.1-2.0 M increases the thermostability of the enzyme with 0.6 and 1.8 M of ammonium sulfate providing complete stability at 60 and 70°C. respectively, 40% of activity is recovered after incubating the purified enzyme at 60°C for 1 h, while there is 12% of activity recovered after incubation at 70°C for 1 h | Aspergillus flavus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.2 | - |
Pectin | - |
Aspergillus flavus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Aspergillus flavus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Aspergillus flavus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzyme is stable for 24 h in the pH range 4.0-10.0 | Aspergillus flavus |