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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
 +
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Cloned

Cloned on EC 4.2.2.10 - pectin lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Escherichia coli
expression in Kluyveromces lactis
expression in Pichia pastoris
expression of mutant enzymes in Aspergillus niger strain NW188, subcloning in Escherichia coli DH5alpha
gene pel2, DNA and amino acid sequence determination and analysis, enzyme contains a signal peptide of 19 amino acids, overexpression under control of the promotor of the Aspergillus oryzae TEF1 gene
-
gene pnl, functional overexpression of the soluble His-tagged enzyme in Escherichia coli BL21(DE3)
-