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Literature summary for 4.2.2.10 extracted from
- Purification and characterization of pectin lyase produced by Aspergillus terricola and its application in retting of natural fibers (2008), Appl. Biochem. Biotechnol., 159, 270-283.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Sodium arsenate | slightly promotes the activity of PNL | Aspergillus terricola |  |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | the purified pectin lyase shows retting ability for natural fibers viz. Cannabis sativa and Linum usitatissimum | Aspergillus terricola |
General Stability
| General Stability | Organism |
|---|---|
| the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase | Aspergillus terricola |
| the purified enzyme retains its full activity after exposure for 1 h at 60°C in 1.0 M ammonium sulfate, in the absence of ammonium sulfate, the enzyme loses its 77% activity at 60°C, at higher temperatures (70-100°C), ammonium sulfate is not able to stabilize the activity of the pectin lyase | Aspergillus terricola |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Aspergillus terricola | |
| Cu2+ | 10% residual activity at 1 mM | Aspergillus terricola | |
| EDTA | 85% residual activity at 1 mM | Aspergillus terricola | |
| Hg2+ | 24% residual activity at 1 mM | Aspergillus terricola | |
| K+ | 91% residual activity at 1 mM; 92% residual activity at 1 mM | Aspergillus terricola | |
| K4[Fe(CN)6] | complete inhibition at 1 mM | Aspergillus terricola | |
| KMnO4 | complete inhibition at 1 mM | Aspergillus terricola | |
| Mg2+ | 97% residual activity at 1 mM | Aspergillus terricola | |
| Mn2+ | 93% residual activity at 1 mM | Aspergillus terricola | |
| potassium ferrocyanide | complete inhibition at 1 mM | Aspergillus terricola | |
| potassium permanganate | complete inhibition at 1 mM | Aspergillus terricola | |
| Sodium azide | 79% residual activity at 1 mM | Aspergillus terricola | |
| Zn2+ | 69% residual activity at 1 mM | Aspergillus terricola | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slightly promotes the activity of PNL (105% relative activity at 1 mM) | Aspergillus terricola | |
| Ca2+ | 105% relative activity at 1 mM | Aspergillus terricola | |
| Co2+ | slightly promotes the activity of PNL (114% relative activity at 1 mM) | Aspergillus terricola | |
| Co2+ | 114% relative activity at 1 mM | Aspergillus terricola | |
| Na+ | slightly promotes the activity of PNL (108.3% relative activity at 1 mM) | Aspergillus terricola | |
| Sodium arsenate | slightly promotes the activity of PNL (110% relative activity at 1 mM) | Aspergillus terricola | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Aspergillus terricola |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus terricola | - |
- |
- |
| Aspergillus terricola MTCC 7588 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE cellulose column chromatography and Sephadex G-100 gel filtration | Aspergillus terricola |
| DEAE-cellulose column chromatography and Sephadex G100 gel filtration | Aspergillus terricola |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| citrus pectin | - |
Aspergillus terricola | ? | - |
? | |
| citrus pectin | - |
Aspergillus terricola MTCC 7588 | ? | - |
? | |
| additional information | pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum | Aspergillus terricola | ? | - |
? | |
| additional information | pectin lyase shows retting ability for natural fibers of Cannabis sativa and Linum usitatissimum | Aspergillus terricola MTCC 7588 | ? | - |
? | |
| pectin | - |
Aspergillus terricola | ? | - |
? | |
| pectin | - |
Aspergillus terricola MTCC 7588 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNL | - |
Aspergillus terricola |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus terricola |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | the enzyme retains its full activity up to 50°C, and thereafter, activity decreases with increase in temperature, 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h, the half-lives at 60, 70, 80, and 85°C are 7.03, 3.9, 1.8, and 0.89 min, respectively | Aspergillus terricola |
| 50 | 60 | the enzyme retains its full activity up to 50°C, and thereafter, activity decreased with increase in temperature, however 75% of the activity is lost after incubating the purified PNL at 60°C for 1 h | Aspergillus terricola |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 110 | - |
citrus pectin | at 37°C | Aspergillus terricola | |
| 110 | - |
Pectin | purified enzyme, at 37°C | Aspergillus terricola | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Aspergillus terricola |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | - |
Aspergillus terricola |
| 4 | 9 | the enzyme retains approximately 100% of its maximum activity for 24 h in a buffer of pH range 4.0-9.0 | Aspergillus terricola |
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