4.2.2.10: pectin lyase
This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.
Word Map on EC 4.2.2.10
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4.2.2.10
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cellulase
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xylanase
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aspergillus
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polysaccharide
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polygalacturonase
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juice
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niger
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pectinolytic
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amylase
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pulp
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peel
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pectate
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penicillium
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citrus
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erwinia
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saccharification
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hemicellulase
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polygalacturonic
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methylesterase
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lyases
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xylan
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bran
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endo-polygalacturonase
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rhamnogalacturonan
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wall-degrading
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glucanase
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enzyme-assisted
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cellulolytic
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carotovora
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pectinesterase
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cellobiase
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tannase
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homogalacturonans
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chrysanthemi
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cmcase
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box-behnken
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3.2.1.15
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nutrition
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synthesis
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fibrobacter
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pomace
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scouring
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industry
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mannanase
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oligogalacturonides
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bagasse
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flavefaciens
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agriculture
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d-galacturonic
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degumming
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food industry
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carbohydrases
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agro-industrial
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ruminicola
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cazymes
- 4.2.2.10
- cellulase
- xylanase
- aspergillus
- polysaccharide
- polygalacturonase
- juice
- niger
-
pectinolytic
- amylase
- pulp
- peel
- pectate
- penicillium
- citrus
- erwinia
-
saccharification
-
hemicellulase
- polygalacturonic
-
methylesterase
- lyases
- xylan
- bran
- endo-polygalacturonase
- rhamnogalacturonan
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wall-degrading
- glucanase
-
enzyme-assisted
-
cellulolytic
- carotovora
- pectinesterase
- cellobiase
- tannase
- homogalacturonans
- chrysanthemi
- cmcase
-
box-behnken
-
3.2.1.15
- nutrition
- synthesis
- fibrobacter
- pomace
-
scouring
- industry
- mannanase
- oligogalacturonides
- bagasse
- flavefaciens
- agriculture
-
d-galacturonic
-
degumming
- food industry
-
carbohydrases
-
agro-industrial
- ruminicola
-
cazymes
Reaction
Synonyms
commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40
ECTree
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Purification
Purification on EC 4.2.2.10 - pectin lyase
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ammonium sulfate fractionation, DEAE cellulose chromatography and Sephadex G-100 gel filtration
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DEAE cellulose column chromatography and Sephadex G-100 gel filtration
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DEAE-cellulose column chromatography and Sephadex G100 gel filtration
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highly methoxylated cross-linked alcohol-insoluble solid column is a simple, inexpensive, convenient, and effective method for pectin lyase purification
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native wild-type enzyme and recombinant mutant enzymes to homogeneity
partial
partial purification of native enzyme, recombinant His-tagged enzyme from Escherichia coli, 113fold to near homogeneity via solubilization and renaturation of inclusion bodies, large DNA fragments prove to be contaminating by binding to the enzyme
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partially
purification by ammonium sulfate fractionation, cation-exchange chromatography on carboxymethyl cellulose followed by gel filtration chromatography
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purification by ammonium sulfate fractionation, gel filtration and ion-exchange chromatography
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recombinant protein, single step purification using His-Trap affinity column chromatography
Sephadex G 100 gel filtration and DEAE-cellulose column chromatography
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