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Literature summary for 4.2.2.10 extracted from

  • Nadaroglu, H.; Taskin, E.; Adiguezel, A.; Guelluece, M.; Demir, N.
    Production of a novel pectin lyase from Bacillus pumilus (P9), purification and characterisation and fruit juice application (2010), Rom. Biotechnol. Lett., 15, 5167-5176.
No PubMed abstract available
Search for more literature for 4.2.2.10

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, 124% of initial activity Bacillus pumilus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 10 mM, 11% of initial activity Bacillus pumilus
EDTA 10 mM, complete loss of activity Bacillus pumilus
Fe2+ 10 mM, 67% of initial activity Bacillus pumilus
Hg2+ 10 mM, complete loss of activity Bacillus pumilus
Mn2+ 10 mM, complete loss ofactivity Bacillus pumilus
additional information not inhibitory: L-cysteine, ascorbic acid Bacillus pumilus
Zn2+ 10 mM, 88% of initial activity Bacillus pumilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Bacillus pumilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, 132% of initial activity Bacillus pumilus
Mg2+ 10 mM, 119% of initial activity Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
-
-
Bacillus pumilus P9
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus pumilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
175
-
 pH 8.0,temperature not specified in the publication Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin
-
 Bacillus pumilus ?
-
 ?
pectin
-
 Bacillus pumilus P9 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 4 h, 40% loss of activity Bacillus pumilus
60
-
 1 h, 50% loss of activity Bacillus pumilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus pumilus