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4.2.2.10: pectin lyase

This is an abbreviated version!
For detailed information about pectin lyase, go to the full flat file.

Word Map on EC 4.2.2.10

Reaction

[6-O-methyl-alpha-D-GalpA]n
=
[6-O-methyl-alpha-D-GalpA]m
 +
4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosy-[6-O-methyl-alpha-D-GalpA]n-m-1

Synonyms

commercial pectic enzyme, CPE, enda-PL, endo PNL, endo-pectin lyase, family 1 pectin lyase A, Forylase PA, lyase, pectin, Macerin G 10X, pectin lyase, pectin lyase 1, pectin lyase A, pectin methyltranseliminase, pectin transeliminase, pectinase, pectinliase, pectinlyase, pectolyase Y23, Pel1, PGL, phytolyase, PL, PL1A, Pla, PLB, PLD, plg1, plg2, PLI, PLII, PNL, pnl-zj5a, Pnl1, Pnl2, PnlH, poly(methoxygalacturonide) lyase, polymethylgalacturonic transeliminase, Rohapect PTE, Ultrazym-100, Ultrazym-20, Ultrazym-40

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.10 pectin lyase

Temperature Stability

Temperature Stability on EC 4.2.2.10 - pectin lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 50
-
stable for 30 min
20
-
room temperature, 5 min, 30% loss of activity
22
-
pH-range 6.0-10, 24 h, more than 85% of initial activity
25
-
t1/2 for soluble enzyme: 6 h, t1/2 for the immobilized enzyme: more than 30 days
29
-
minimum deactivation rate constant at pH 3.9 and 29°C, deactivation process is modelled as first-order kinetics
35
-
t1/2 for soluble enzyme: 1 h, t1/2 for the immobilized enzyme: more than 22 days
36
-
10 min, 30% loss of activity
42
-
5 min, 30% loss of activity
43
-
1 h, pH 5.0, 77% residual activity
45 - 50
-
the pectin lyase activity shows thermal stability, with half-lives of 30 and 27 min when incubated at 45 and 50°C, respectively
50 - 60
50 - 70
-
the enzyme does not loose activity up to 50°C if exposed for 1 h, addition of ammonium sulfate in the range of 0.1-2.0 M increases the thermostability of the enzyme with 0.6 and 1.8 M of ammonium sulfate providing complete stability at 60 and 70°C. respectively, 40% of activity is recovered after incubating the purified enzyme at 60°C for 1 h, while there is 12% of activity recovered after incubation at 70°C for 1 h
55
-
half-life 5.71 and 80 h for pectin lyase produced in synthetic and agro-industrial medium, respectively
68
-
pH 7.8, 5 min, 97% loss of activity
8
-
stable for 1 h
additional information