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4.2.1.78: (S)-norcoclaurine synthase

This is an abbreviated version!
For detailed information about (S)-norcoclaurine synthase, go to the full flat file.

Word Map on EC 4.2.1.78

Reaction

4-hydroxyphenylacetaldehyde
+
dopamine
=
(S)-norcoclaurine
 +
H2O

Synonyms

(S)-norcoclaurine synthase, (S)-Norlaudanosoline synthase, AmNCS1, AmNCS2, CCHNCS2, CjNCS1, CjPR10A, CsNCS, More, NCS, NCS1, NCS2, NDONCS3, norcoclaurine synthase, PbNCS, PR10A, PSONCS1, PSONCS2, S-norcoclaurine synthase, Synthase, (S)-norlaudanosoline, TFLNCSDELTA25, TfNCS

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.78 (S)-norcoclaurine synthase

Cloned

Cloned on EC 4.2.1.78 - (S)-norcoclaurine synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli and in Saccharomyces cereviseae
expression in Escherichia coli BL21 (DE3)
expression in Escherichia coli BL21 (DE3). Under certain conditions lyophilized whole Escherichia coli cells containing the overexpressed enzyme turn out to a suitable catalysts
expression in Escherichia coli is optimized
-
expression of CjNCS1 in Escherichia coli
-
expression of CjPR10A in Escherichia coli
-
N-terminally truncated enzyme (CjNCS-DELTA29, with a deletion of the first 29 amino acid residues) is expressed in Escherichia coli. The cell-free extract of Escherichia coli expressing CjNCS-DELTA29 exhibits 40fold higher activity than that expressing intact enzyme
NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole
-
overexpressed in Escherichia coli as a recombinant His-tagged enzyme
-
recombinant expression of C-terminally His-tagged NCS in Eschericha coli strain BL21(DE3)
-
the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates
the NCS protein truncated at the first 19 amino acids, with a N-terminal MTGS sequence and a His tag at the C terminus is subcloned into the NdeI and XhoI restriction site of the vector pET22-b. Transformation of chemically competent Escherichia coli strain BL21DE3
unlabelled and 15N-labelled delta 29NCS is expressed using the expression system Escherichia coli Rosetta(DE3)/pET29b-delta 29NCS
-