BRENDA - Enzyme Database
show all sequences of 4.2.1.78

Cloning, expression, crystallization and preliminary X-ray data analysis of norcoclaurine synthase from Thalictrum flavum

Pasquo, A.; Bonamore, A.; Franceschini, S.; Macone, A.; Boffi, A.; Ilari, A.; Acta Crystallogr. Sect. F 64, 281-283 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates
Thalictrum flavum
Crystallization (Commentary)
Crystallization
Organism
at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 A resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3121, with unit-cell parameters a = b = 86.31, c = 118.36 A. A selenomethionine derivate is crystallized in the same space group, 2.7 A resolution
Thalictrum flavum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
Thalictrum flavum
-
(S)-Norcoclaurine + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thalictrum flavum
Q67A25
-
-
Purification (Commentary)
Commentary
Organism
HisTrap Fast Flow column
Thalictrum flavum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
-
690258
Thalictrum flavum
(S)-Norcoclaurine + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates
Thalictrum flavum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
at 294 K by the hanging-drop vapour-diffusion method using ammonium sulfate and sodium chloride as precipitant agents and diffract to better than 3.0 A resolution using a synchrotron-radiation source. The crystals belong to the trigonal space group P3121, with unit-cell parameters a = b = 86.31, c = 118.36 A. A selenomethionine derivate is crystallized in the same space group, 2.7 A resolution
Thalictrum flavum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
Thalictrum flavum
-
(S)-Norcoclaurine + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
HisTrap Fast Flow column
Thalictrum flavum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde
-
690258
Thalictrum flavum
(S)-Norcoclaurine + H2O
-
-
-
?
Other publictions for EC 4.2.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747372
Lechner
Library of norcoclaurine synt ...
Argemone mexicana, Corydalis saxicola, Papaver bracteatum
Biotechnol. J.
13
e1700542
2018
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3
3
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8
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3
4
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747130
Lichman
Structural evidence for the d ...
Thalictrum flavum
Biochemistry
56
5274-5277
2017
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1
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748745
Lichman
Enzyme catalysed Pictet-Speng ...
Thalictrum flavum
Nat. Commun.
8
14883
2017
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1
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2
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749319
Vimolmangkang
Evolutionary origin of the NC ...
Nelumbo nucifera
Sci. Rep.
6
26323
2016
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2
2
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749327
Li
Genes encoding norcoclaurine ...
Corydalis chinensis, Nandina domestica, Papaver somniferum, Thalictrum flavum
Sci. Rep.
6
39256
2016
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-
4
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4
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5
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10
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10
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4
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4
5
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747310
Nishihachijo
Asymmetric synthesis of tetra ...
Coptis japonica
Biosci. Biotechnol. Biochem.
78
701-707
2014
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1
1
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1
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1
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1
1
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728793
De la Cruz-Chacon
-
Activities of enzymes catalyzi ...
Annona macroprophyllata
Russ. J. Plant Physiol.
60
791-799
2013
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1
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729604
Zhang
-
Molecular cloning and heterolo ...
Arabidopsis thaliana
Chin. J. Appl. Environ. Biol.
19
61-68
2013
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1
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730988
Ruff
Biocatalytic production of tet ...
Thalictrum flavum
Tetrahedron Lett.
53
1071-1074
2012
-
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1
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1
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1
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1
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16
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16
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705406
Michalska
Crystal structure of Hyp-1, a ...
Hypericum perforatum
J. Struct. Biol.
169
161-171
2010
-
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715141
Bonamore
-
An enzymatic, stereoselective ...
Thalictrum flavum
Green Chem.
12
1623-1627
2010
-
1
1
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1
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1
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716518
Lee
Norcoclaurine synthase is a me ...
Coptis japonica, Papaver somniferum, Thalictrum flavum
Plant Cell
22
3489-3503
2010
-
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1
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1
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1
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3
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3
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6
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10
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4
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10
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4
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1
6
6
1
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693259
Ilari
Structural basis of enzymatic ...
Thalictrum flavum
J. Biol. Chem.
284
897-904
2009
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1
1
4
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3
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1
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2
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2
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3
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690258
Pasquo
Cloning, expression, crystalli ...
Thalictrum flavum
Acta Crystallogr. Sect. F
64
281-283
2008
-
-
1
1
-
-
-
-
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1
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2
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1
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690826
Berkner
Conformation, catalytic site, ...
Thalictrum flavum
Biochem. J.
413
281-290
2008
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1
1
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4
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678219
Luk
Mechanistic studies on norcocl ...
Thalictrum flavum
Biochemistry
46
10153-10161
2007
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2
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680879
Minami
Functional analysis of norcocl ...
Coptis japonica
J. Biol. Chem.
282
6274-6282
2007
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1
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682682
Berkner
High-yield expression and puri ...
Thalictrum flavum
Protein Expr. Purif.
56
197-204
2007
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652296
Samanani
Purification and characterizat ...
Thalictrum flavum ssp. glaucum
J. Biol. Chem.
277
33878-33883
2002
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1
2
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1
2
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1
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1
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1
1
1
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1
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4
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653569
Samanani
Isolation and partial characte ...
Eschscholzia californica, no activity in Catharanthus roseus, no activity in Nicotiana tabacum, Papaver somniferum, Thalictrum flavum ssp. glaucum
Planta
213
898-906
2001
2
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1
6
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3
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11
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3
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8
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6
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1
1
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1
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1
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6
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8
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2
6
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3
1
1
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3
1
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5895
Schumacher
Partial purification and prope ...
Eschscholtzia tenuifolia
J. Med. Plant Res.
48
212-220
1983
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1
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1
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1
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2
1
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5894
Rueffer
-
(S)-Norlaudanosoline synthase: ...
Berberis regeliana, Capnoides sempervirens, Eschscholtzia tenuifolia, Eschscholzia pulchella, Fumaria officinalis, Glaucium rubrum, Lamprocapnos spectabilis, Meconopsis cambrica, Papaver somniferum, Ranunculus flammula, Thalictrum minus, Thalictrum tuberosum
FEBS Lett.
129
5-9
1981
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2
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12
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12
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12
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25
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1
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12
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12
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25
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1
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