Literature summary for 4.2.1.78 extracted from

Berkner, H.; Schweimer, K.; Matecko, I.; Roesch, P.
Conformation, catalytic site, and enzymatic mechanism of the PR10 allergen-related enzyme norcoclaurine synthase (2008), Biochem. J., 413, 281-290.

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Cloned(Commentary)

Cloned (Comment)	Organism
unlabelled and 15N-labelled delta 29NCS is expressed using the expression system Escherichia coli Rosetta(DE3)/pET29b-delta 29NCS	Thalictrum flavum

Crystallization (Commentary)

Crystallization (Comment)	Organism
combining experimental NMR spectroscopic data with homology modelling using the homologous major birch pollen allergen Bet v 1 (PDB code 1BV1) as template structure a reliable model structure of delta 29NCS is built	Thalictrum flavum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde	Thalictrum flavum	-	(S)-Norcoclaurine + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Thalictrum flavum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thalictrum flavum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde	-	Thalictrum flavum	(S)-Norcoclaurine + H2O	-	?

Subunits

Subunits	Comment	Organism
More	size-exclusion chromatography (SEC) and NMR relaxation measurements, concentration-dependent oligomerization of delta 29NCS with an equilibrium of monomeric and oligomeric protein over a broad concentration range, at low protein concentrations in the range of 10 microM the enzyme is predominantly monomeric, no indication that substrate binding induces dimerization of delta 29NCS	Thalictrum flavum

Synonyms

Synonyms	Comment	Organism
(S)-norcoclaurine synthase	-	Thalictrum flavum
NCS	-	Thalictrum flavum

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Release 2023.1 (January 2023)