BRENDA - Enzyme Database
show all sequences of 4.2.1.78

Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family

Lee, E.J.; Facchini, P.; Plant Cell 22, 3489-3503 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole
Papaver somniferum
Engineering
Amino acid exchange
Commentary
Organism
additional information
virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS
Papaver somniferum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
immunoprecipitation of NCS from total protein extracts of Thalictrum flavum cells results in a nearly complete attenuation of NCS activity
Thalictrum flavum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
immunolocalization study of NCS, overview
Papaver somniferum
-
-
soluble
-
Thalictrum flavum
-
-
vacuole
NCS2 has an N-terminal signal peptide and sorts to the vacuole
Papaver somniferum
5773
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Papaver somniferum
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Coptis japonica
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Thalictrum flavum
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Coptis japonica
-
-
-
Papaver somniferum
-
isozymes NCS1 and NCS2
-
Thalictrum flavum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
carpel
low level
Papaver somniferum
-
cell culture
-
Thalictrum flavum
-
flower bud
very low expression level
Thalictrum flavum
-
leaf
low level
Papaver somniferum
-
additional information
not in leaves
Thalictrum flavum
-
phloem
NCS2 shows cell type-specific occurrence in phloem sieve elements, colocalization with all other known benzylisoquinoline alkaloid biosynthetic enzymes
Papaver somniferum
-
rhizome
-
Thalictrum flavum
-
root
abundant
Papaver somniferum
-
root
-
Thalictrum flavum
-
stem
abundant
Papaver somniferum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Papaver somniferum
(S)-norcoclaurine + H2O
-
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Coptis japonica
(S)-norcoclaurine + H2O
-
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Thalictrum flavum
(S)-norcoclaurine + H2O
-
-
-
?
additional information
isozyme NCS1 of Coptis japonica does not catalyze the formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde
716518
Coptis japonica
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole
Papaver somniferum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
virus-induced gene silencing of NCS in opium poppy profoundly reduces alkaloid levels compared to controls, a mixed culture of Agrobacterium tumefaciens harboring either pTRV2-PsNCS or pTRV1 is infiltrated into opium poppy seedlings tow weeks after germination. Levels of the five major alkaloids in opium poppy latex, i.e. morphine, codeine, thebaine, noscapine, and papaveine, are reduced by 75-82% in plants infiltrated with Agrobacterium tumefaciens harboring pTRV2-PsNCS
Papaver somniferum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
immunoprecipitation of NCS from total protein extracts of Thalictrum flavum cells results in a nearly complete attenuation of NCS activity
Thalictrum flavum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
additional information
immunolocalization study of NCS, overview
Papaver somniferum
-
-
soluble
-
Thalictrum flavum
-
-
vacuole
NCS2 has an N-terminal signal peptide and sorts to the vacuole
Papaver somniferum
5773
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Papaver somniferum
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Coptis japonica
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
Thalictrum flavum
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
(S)-norcoclaurine + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
carpel
low level
Papaver somniferum
-
cell culture
-
Thalictrum flavum
-
flower bud
very low expression level
Thalictrum flavum
-
leaf
low level
Papaver somniferum
-
additional information
not in leaves
Thalictrum flavum
-
phloem
NCS2 shows cell type-specific occurrence in phloem sieve elements, colocalization with all other known benzylisoquinoline alkaloid biosynthetic enzymes
Papaver somniferum
-
rhizome
-
Thalictrum flavum
-
root
abundant
Papaver somniferum
-
root
-
Thalictrum flavum
-
stem
abundant
Papaver somniferum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Papaver somniferum
(S)-norcoclaurine + H2O
-
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Coptis japonica
(S)-norcoclaurine + H2O
-
-
-
?
4-hydroxyphenylacetaldehyde + 4-(2-aminoethyl)benzene-1,2-diol
4-(2-aminoethyl)benzene-1,2-diol, i.e. dopamine
716518
Thalictrum flavum
(S)-norcoclaurine + H2O
-
-
-
?
additional information
isozyme NCS1 of Coptis japonica does not catalyze the formation of (S)-norcoclaurine from dopamine and 4-hydroxyphenylacetaldehyde
716518
Coptis japonica
?
-
-
-
-
Expression
Organism
Commentary
Expression
Papaver somniferum
elicitor-induced cell cultures show increasing expression of NCS after 2 to 100 hours
up
General Information
General Information
Commentary
Organism
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Coptis japonica
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Papaver somniferum
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Thalictrum flavum
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Coptis japonica
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Papaver somniferum
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Thalictrum flavum
General Information (protein specific)
General Information
Commentary
Organism
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Coptis japonica
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Papaver somniferum
metabolism
norcoclaurine synthase, NCS, catalyzes the first committed step in the biosynthesis of benzylisoquinoline alkaloids, exclusive role of PR10/Bet v1-type NCS enzymes, overview
Thalictrum flavum
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Coptis japonica
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Papaver somniferum
additional information
amino acid sequence comparisons of NCSs from different species, phylogenetic tree, overview
Thalictrum flavum
Expression (protein specific)
Organism
Commentary
Expression
Papaver somniferum
elicitor-induced cell cultures show increasing expression of NCS after 2 to 100 hours
up
Other publictions for EC 4.2.1.78
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
747372
Lechner
Library of norcoclaurine synt ...
Argemone mexicana, Corydalis saxicola, Papaver bracteatum
Biotechnol. J.
13
e1700542
2018
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3
3
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8
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3
4
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747130
Lichman
Structural evidence for the d ...
Thalictrum flavum
Biochemistry
56
5274-5277
2017
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1
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1
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3
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1
1
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748745
Lichman
Enzyme catalysed Pictet-Speng ...
Thalictrum flavum
Nat. Commun.
8
14883
2017
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1
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2
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2
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1
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749319
Vimolmangkang
Evolutionary origin of the NC ...
Nelumbo nucifera
Sci. Rep.
6
26323
2016
-
-
-
-
-
-
-
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3
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2
2
-
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749327
Li
Genes encoding norcoclaurine ...
Corydalis chinensis, Nandina domestica, Papaver somniferum, Thalictrum flavum
Sci. Rep.
6
39256
2016
-
-
4
-
-
-
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4
-
-
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5
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10
-
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10
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4
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5
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5
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10
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4
-
-
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4
5
-
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747310
Nishihachijo
Asymmetric synthesis of tetra ...
Coptis japonica
Biosci. Biotechnol. Biochem.
78
701-707
2014
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1
1
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-
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1
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3
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1
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1
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1
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1
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4
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1
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1
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1
1
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728793
De la Cruz-Chacon
-
Activities of enzymes catalyzi ...
Annona macroprophyllata
Russ. J. Plant Physiol.
60
791-799
2013
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1
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1
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729604
Zhang
-
Molecular cloning and heterolo ...
Arabidopsis thaliana
Chin. J. Appl. Environ. Biol.
19
61-68
2013
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1
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730988
Ruff
Biocatalytic production of tet ...
Thalictrum flavum
Tetrahedron Lett.
53
1071-1074
2012
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16
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705406
Michalska
Crystal structure of Hyp-1, a ...
Hypericum perforatum
J. Struct. Biol.
169
161-171
2010
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715141
Bonamore
-
An enzymatic, stereoselective ...
Thalictrum flavum
Green Chem.
12
1623-1627
2010
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1
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1
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1
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716518
Lee
Norcoclaurine synthase is a me ...
Coptis japonica, Papaver somniferum, Thalictrum flavum
Plant Cell
22
3489-3503
2010
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1
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1
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1
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3
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6
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10
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10
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4
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1
6
6
1
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693259
Ilari
Structural basis of enzymatic ...
Thalictrum flavum
J. Biol. Chem.
284
897-904
2009
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4
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3
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690258
Pasquo
Cloning, expression, crystalli ...
Thalictrum flavum
Acta Crystallogr. Sect. F
64
281-283
2008
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1
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690826
Berkner
Conformation, catalytic site, ...
Thalictrum flavum
Biochem. J.
413
281-290
2008
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1
1
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1
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4
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678219
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Thalictrum flavum
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4
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680879
Minami
Functional analysis of norcocl ...
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4
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1
1
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4
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653569
Samanani
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Eschscholzia californica, no activity in Catharanthus roseus, no activity in Nicotiana tabacum, Papaver somniferum, Thalictrum flavum ssp. glaucum
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6
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8
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6
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1
1
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3
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Schumacher
Partial purification and prope ...
Eschscholtzia tenuifolia
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1983
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1
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5894
Rueffer
-
(S)-Norlaudanosoline synthase: ...
Berberis regeliana, Capnoides sempervirens, Eschscholtzia tenuifolia, Eschscholzia pulchella, Fumaria officinalis, Glaucium rubrum, Lamprocapnos spectabilis, Meconopsis cambrica, Papaver somniferum, Ranunculus flammula, Thalictrum minus, Thalictrum tuberosum
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