Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli as a recombinant His-tagged enzyme | Thalictrum flavum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.288 | - |
4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | |
335 | - |
(3,4-dihydroxyphenyl)acetaldehyde | - |
Thalictrum flavum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalictrum flavum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme | Thalictrum flavum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2-aminoethyl)phenol + 4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | ? | - |
? | |
4-(2-aminoethyl)benzene-1,2-diol + (3,4-dihydroxyphenyl)acetaldehyde | - |
Thalictrum flavum | ? | - |
? | |
4-(2-Aminoethyl)benzene-1,2-diol + 4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | (S)-Norcoclaurine + H2O | - |
? | |
5-(2-aminoethyl)-2-methoxyphenol + 4-hydroxyphenylacetaldehyde | - |
Thalictrum flavum | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
4-hydroxyphenylacetaldehyde | cosubstrate: 3-(2-aminoethyl)phenol | Thalictrum flavum | |
6 | - |
3-(2-aminoethyl)phenol | cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum | |
6 | - |
5-(2-aminoethyl)-2-methoxyphenol | cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum | |
6 | - |
4-hydroxyphenylacetaldehyde | cosubstrate: 5-(2-aminoethyl)-2-methoxyphenol | Thalictrum flavum | |
6.4 | - |
4-hydroxyphenylacetaldehyde | cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum | |
6.4 | - |
4-(2-Aminoethyl)benzene-1,2-diol | cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum | |
7 | - |
4-(2-Aminoethyl)benzene-1,2-diol | cosubstrate: (3,4-dihydroxyphenyl)acetaldehyde | Thalictrum flavum | |
7 | - |
(3,4-dihydroxyphenyl)acetaldehyde | cosubstrate: 4-(2-aminoethyl)benzene-1,2-diol | Thalictrum flavum |