3.6.1.23: dUTP diphosphatase

This is an abbreviated version!
For detailed information about dUTP diphosphatase, go to the full flat file.

Reaction

Synonyms

DCD-DUT, dCTP deaminase-dUTPase, dCTP deaminase:dUTPase, deoxyuridine 5'-triphosphate, deoxyuridine 5'-triphosphate nucleotide hydrolase, deoxyuridine 5'-triphosphate nucleotidohydrolase, deoxyuridine 5'-triphosphate pyrophosphatase, deoxyuridine 50-triphosphate nucleotidohydrolase, deoxyuridine 5�-triphosphate nucleotidohydrolase, deoxyuridine nucleotidohydrolase, deoxyuridine triphosphatase, deoxyuridine triphosphate nucleotidohydrolase, deoxyuridine triphosphate pyrophosphatase, deoxyuridine-5'-triphosphate nucleotidohydrolase, deoxyuridine-triphosphate pyrophosphatase, desoxyuridine 5'-triphosphatase, desoxyuridine 5'-triphosphate nucleotidohydrolase, desoxyuridine-triphosphatase, DURP, DUT, DUT-N, DUT1, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPae, dUTPase, dUTPase-dUDPase, dUTPase-related protein, E165R, More, ORF 54, ORF8, P18, PIP4, Pr95, STSV2_30, Tb927.7.5160, U45, YncF, YosS

ECTree

     3 Hydrolases

         3.6 Acting on acid anhydrides

             3.6.1 In phosphorus-containing anhydrides

                3.6.1.23 dUTP diphosphatase

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Results	in table
2	Activating Compound
33833	AA Sequence
32	Application
1	CAS Registry Number
73	Cloned(Commentary)
44	Crystallization (Commentary)
670	Disease/ Diagnostics
2	Expression
22	General Information
2	General Stability
88	IC50 Value
352	Inhibitors
62	kcat/KM [mM/s]
173	Ki Value [mM]
139	KM Value [mM]
26	Localization
89	Metals/Ions
65	Molecular Weight [Da]
135	Natural Substrates/ Products (Substrates)
487	Organism
10	Pathway
409	PDB ID
23	pH Optimum
12	pH Range
1	pH Stability
5	pI Value
1	Posttranslational Modification
99	Protein Variants
71	Purification (Commentary)
19	Reaction
1	Reaction Type
210	Reference
50	Source Tissue
20	Specific Activity [micromol/min/mg]
8	Storage Stability
329	Substrates and Products (Substrate)
92	Subunits
261	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
5	Temperature Range [°C]
19	Temperature Stability [°C]
95	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 3.6.1.23 - dUTP diphosphatase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

110 - 600

2'-deoxy-5-fluoro-UTP

2 entries

0.00525 - 0.0061

beta-L-2'-dUTP

2 entries

0.00265 - 16

dATP

2 entries

0.02172 - 21

dCTP

3 entries

0.00013 - 14

dGTP

2 entries

29

dITP

Saccharomyces cerevisiae

P33317

wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787

0.0092 - 13

dTTP

3 entries

6 - 10000

dUTP

44 entries

0.01121 - 150

UTP

3 entries

110

2'-deoxy-5-fluoro-UTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

600

2'-deoxy-5-fluoro-UTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.00525

beta-L-2'-dUTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.0061

beta-L-2'-dUTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.00265

dATP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

16

dATP

Dugesia ryukyuensis

-

in the presence of 5 mM Mg2+, at pH 8.2 and 25°C

756316

0.02172

dCTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.1616

dCTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

21

dCTP

Dugesia ryukyuensis

-

in the presence of 5 mM Mg2+, at pH 8.2 and 25°C

756316

0.00013

dGTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

14

dGTP

Dugesia ryukyuensis

-

in the presence of 5 mM Mg2+, at pH 8.2 and 25°C

756316

0.0092

dTTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.01971

dTTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

13

dTTP

Dugesia ryukyuensis

-

in the presence of 5 mM Mg2+, at pH 8.2 and 25°C

756316

6

dUTP

Human gammaherpesvirus 4

P03195

mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

8

dUTP

Human gammaherpesvirus 4

P03195

mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

8

dUTP

Human gammaherpesvirus 4

P03195

mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

9.8

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R161K, at pH 7.5 and 20°C

757196

20

dUTP

Human gammaherpesvirus 4

P03195

mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

32

dUTP

white spot syndrome virus

Q77J78

mutant enzyme DELTA134-153, at pH 7.5 and 20°C

757196

62

dUTP

Homo sapiens

-

mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C

713147

71

dUTP

white spot syndrome virus

Q77J78

mutant enzyme D34N, at pH 7.5 and 20°C

757196

74

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C

757196

75

dUTP

white spot syndrome virus

Q77J78

mutant enzyme D170L, at pH 7.5 and 20°C

757196

76

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R24T/S115R, at pH 7.5 and 20°C

757196

90

dUTP

Saccharomyces cerevisiae

P33317

mutant enzyme Q114A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787

100

dUTP

Saccharomyces cerevisiae

P33317

mutant enzyme R111A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787

160

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R24T, at pH 7.5 and 20°C

757196

180

dUTP

Saccharomyces cerevisiae

P33317

mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787

200

dUTP

Human gammaherpesvirus 4

P03195

wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

240

dUTP

white spot syndrome virus

Q77J78

mutant enzyme F166Y, at pH 7.5 and 20°C

757196

250

dUTP

Escherichia coli

-

mutant enzyme T22R/R116S, at pH 7.5 and 20°C

757196

520

dUTP

Escherichia coli

-

mutant enzyme R166S, at pH 7.5 and 20°C

757196

580

dUTP

white spot syndrome virus

Q77J78

mutant enzyme S115R, at pH 7.5 and 20°C

757196

610

dUTP

white spot syndrome virus

Q77J78

mutant enzyme DELTA170-171, at pH 7.5 and 20°C

757196

740

dUTP

Saccharomyces cerevisiae

P33317

wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787

970

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R158W, at pH 7.5 and 20°C

757196

1000

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R158E, at pH 7.5 and 20°C

757196

1200

dUTP

white spot syndrome virus

Q77J78

mutant enzyme R158A, at pH 7.5 and 20°C

757196

1800

dUTP

Human gammaherpesvirus 4

P03195

wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

1900

dUTP

Homo sapiens

-

mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C

713147

2100

dUTP

white spot syndrome virus

Q77J78

mutant enzyme K150E, at pH 7.5 and 20°C

757196

2300

dUTP

Human gammaherpesvirus 4

P03195

wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

2400

dUTP

white spot syndrome virus

Q77J78

mutant enzyme K150A, at pH 7.5 and 20°C

757196

2800

dUTP

Human gammaherpesvirus 4

P03195

mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C

712381

3000

dUTP

white spot syndrome virus

Q77J78

mutant enzyme D170G, at pH 7.5 and 20°C

757196

3700

dUTP

white spot syndrome virus

Q77J78

mutant enzyme V160D, at pH 7.5 and 20°C

757196

4400

dUTP

white spot syndrome virus

Q77J78

wild type enzyme, at pH 7.5 and 20°C

757196

5800

dUTP

Homo sapiens

-

wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C

713147

6100

dUTP

Plasmodium falciparum

Q8II92

mutant enzyme K96A, at pH 8.0 and 25°C

719408

6300

dUTP

Plasmodium falciparum

Q8II92

mutant enzyme F46A, at pH 8.0 and 25°C

719408

6500

dUTP

Dugesia ryukyuensis

-

in the presence of 5 mM Mn2+, at pH 8.2 and 25°C

756316

6600

dUTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

7100

dUTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

7100

dUTP

Plasmodium falciparum

Q8II92

mutant enzyme I117A, at pH 8.0 and 25°C

719408

7300

dUTP

Plasmodium falciparum

Q8II92

wild type enzyme, at pH 8.0 and 25°C

719408

8500

dUTP

Dugesia ryukyuensis

-

in the presence of 5 mM Mg2+, at pH 8.2 and 25°C

756316

10000

dUTP

Escherichia coli

-

wild type enzyme, at pH 7.5 and 20°C

757196

0.01121

UTP

Plasmodium falciparum

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

0.01278

UTP

Homo sapiens

-

at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0

711327

150

UTP

Saccharomyces cerevisiae

P33317

mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C

718787