Reference on EC 3.6.1.23 - dUTP diphosphatase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Neale, G.A.M.; Mitchell, A.; Finch, L.R.
Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides
J. Bacteriol.
156
1001-1005
1983
Mycoplasma mycoides
Greenberg, R.G.; Somerville, R.L.
Deoxyuridylate kinase activity and deoxyuridinetriphosphatase in Escherichia coli
Biochemistry
48
247-257
1962
Escherichia coli, Escherichia coli R2
Bertani, L.E.; Haeggmark, A.; Reichard, P.
Enzymatic synthesis of deoxyribonucleotides. II. Formation and interconversion of deoxyuridine phosphates
J. Biol. Chem.
238
3407-3413
1963
Escherichia coli, Escherichia coli B / ATCC 11303
Grindey, G.B.; Nichol, Ch.A.
Mammalian deoxyuridine 5-triphosphate pyrophosphatase
Biochim. Biophys. Acta
240
180-183
1971
Mus musculus
Price, A.R.; Frato, J.
Bacillus subtilis deoxyuridinetriphosphatase and its bacteriophage PBS2-induced inhibitor
J. Biol. Chem.
250
8804-8811
1975
Bacillus subtilis
Shlomai, J.; Kornberg, A.
Deoxyuridine triphosphatase of Escherichia coli. Purification, properties, and use as a reagent to reduce uracil incorporation into DNA
J. Biol. Chem.
253
3305-3312
1978
Escherichia coli
Williams, M.V.; Cheng, Y.
Human deoxyuridine triphosphate nucleotidohydrolase. Purification and characterization of the deoxyuridine triphosphate nucleotidohydrolase from acute lymphocytic leukemia
J. Biol. Chem.
254
2897-2901
1979
Homo sapiens
Wist, E.
Partial purification of deoxyuridine triphosphate nucleotidohydrolase and its effect on DNA synthesis in isolated HeLa cell nuclei
Biochim. Biophys. Acta
565
98-106
1979
Homo sapiens
Taylor, A.F.; Siliciano, P.G.; Weiss, B.
Cloning of the dut (deoxyuridine triphosphatase) gene of Escherichia coli
Gene
9
321-336
1980
Escherichia coli
Hokari, S.; Sakagishi, Y.; Tsukada, K.
Enhanced activity of deoxyuridine 5'-triphosphatase in regenerating rat liver
Biochem. Biophys. Res. Commun.
108
95-101
1982
Rattus norvegicus
Ingraham, H.A.; Goulian, M.
Deoxyuridine triphosphatase: a potential site of interaction with pyrimidine nucleotide analogues
Biochem. Biophys. Res. Commun.
109
746-752
1982
Homo sapiens
Lundberg, L.G.; Karlstroem, O.H.; Nyman, P.O.; Neuhard, J.
Isolation and characterization of the dut gene of Escherichia coli. I. Cloning in thermoinducible plasmids
Gene
22
115-126
1983
Escherichia coli
Caradonna, S.J.; Adamkiewicz, D.M.
Purification and properties of the deoxyuridine triphosphate nucleotidohydrolase enzyme derived from HeLa S3 cells. Comparison to a distinct dUTP nucleotidohydrolase induced in herpes simplex virus-infected HeLa S3 cells
J. Biol. Chem.
259
5459-5464
1984
Human alphaherpesvirus 1, Homo sapiens
Williams, M.V.; Pollack, J.D.
Purification and characterization of a dUTPase from Acholeplasma laidlawii B-PG9
J. Bacteriol.
159
278-282
1984
Acholeplasma laidlawii, Acholeplasma laidlawii B-PG9
Williams, M.V.
Deoxyuridine triphosphate nucleotidohydrolase induced by herpes simplex virus type 1. Purification and characterization of induced enzyme
J. Biol. Chem.
259
10080-10084
1984
Human alphaherpesvirus 1, Homo sapiens
Williams, M.V.; Pollack, J.D.
Pyrimidine deoxyribonucleotide metabolism in Acholeplasma laidlawii B-PG9
J. Bacteriol.
161
1029-1033
1985
Acholeplasma laidlawii, Acholeplasma laidlawii B-PG9
Williams, M.V.; Holliday, J.; Glaser, R.
Induction of a deoxyuridine triphosphate nucleotidohydrolase activity in Epstein-Barr virus-infected cells
Virology
142
326-333
1985
Human gammaherpesvirus 4
Giroir, L.E.; Deutsch, W.A.
Drosophila deoxyuridine triphosphatase. Purification and characterization
J. Biol. Chem.
262
130-134
1987
Drosophila melanogaster
Williams, M.V.; Parris, D.S.
Characterization of a herpes simplex virus type 2 deoxyuridine triphosphate nucleotidohydrolase and mapping of a gene conferring type specificity for the enzyme
Virology
156
282-292
1987
Human alphaherpesvirus 2
Hoffmann, I.; Widstroem, J.; Zeppezauer, M.; Nyman, P.O.
Overproduction and large-scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli
Eur. J. Biochem.
164
45-51
1987
Escherichia coli, Escherichia coli overproducing
Hokari, S.; Sakagishi, Y.
Purification and characterization of deoxyuridine triphosphate nucleotidohydrolase from anemic rat spleen: a trimer composition of the enzyme protein
Arch. Biochem. Biophys.
253
350-356
1987
Rattus norvegicus
El-Hajj, H.H.; Zhang, H.; Weiss, B.
Lethality of a dut (deoxyuridine triphosphatase) mutation in Escherichia coli
J. Bacteriol.
170
1069-1075
1988
Escherichia coli
Dauter, Z.; Persson, R.; Rosengren, A.M.; Nyman, P.O.; Wilson, K.S.; Cedergren-Zeppezauer, E.S.
Crystal structure of UTPase from equine infectious anaemia virus: active site metal binding in a substrate analogue complex
J. Mol. Biol.
15
655-673
1999
equine infectious anemia virus
Prasad, G.S.; McRhee, D.E.; Laco, G.S.; Hasselkus-Light, C.; Elder, J.H.; Stout, C.D.
Crystal structure of dUTP pyrophosphatase from feline immunodeficiency virus
Protein Sci.
5
2429-2437
1996
feline immunodeficiency virus
Larsson, G.; Svensson, L.A.; Nyman, P.O.
Crystal structure of the Escherichia coli dUTP in complex with a substrate analogue (dUDP)
Nature Struct. Biol.
3
532-538
1996
Escherichia coli
Camacho, A.; Hilgado-Zarco, F.; Bernier-Villamor, V.; Ruiz-PeRez, L.M.; Gonzalez-Pacanowska, D.
Properties of Leishmania major dUTP nucleotidohydrolase, a distinct nucleotide-hydrolysing enzyme in kinetoplastids
Biochem. J.
346
163-168
2000
Leishmania major
Larsson, G.; Nyman, P.O.; Kvassman, J.O.
Kinetic characterization of dUTPase from Escherichia coli
J. Biol. Chem.
271
24010-24016
1996
Escherichia coli
Roseman, N.A.; Evans, R.K.; Mayer, E.L.; Rossi, M.A.; Slabaugh, M.B.
Purification and characterization of the vaccinia virus deoxyuridine triphosphatase expressed in Escherichia coli
J. Biol. Chem.
271
23506-23511
1996
Vaccinia virus
Ladner, R.D.; McNulty, D.E.; Carr, S.A.; Roberts, G.D.; Caradonna, S.J.
Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase
J. Biol. Chem.
271
7745-7751
1996
Homo sapiens (P33316), Homo sapiens
Bernier-Villamor, V.; Camacho, A; Gonzalez-Pacanowska, D.; Cedergren-Zeppezauer, E.; Antson, A.; Wilson, K.S.
Crystallization and preliminary X-ray diffraction of Trypanosoma cruzi dUTPase
Acta Crystallogr. Sect. D
55
528-530
1999
Trypanosoma cruzi
Nord, J.; Larsson, G.;Kvassman, J.O.; Rosengren, A.M.; Nyman, P.O.
dUTPase from the retrovirus equine infectious anemia virus: specificity, turnover and inhibition
FEBS Lett.
414
271-274
1997
equine infectious anemia virus
Harris, J.M.; McIntosh, E.M.; Muscat, G.E.O.
Structure/function analysis of a dUTPase: catalytic mechanism of a potential chemotherapeutic target
J. Mol. Biol.
288
275-287
1999
human endogenous retrovirus
McIntosh, E.M.; Ager, D.D.; Gadsen, M.H.; Haynes, R.H.
Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme
Proc. Natl. Acad. Sci. USA
89
8020-8024
1992
Homo sapiens
Shao, H.; Robek, M.D.; Threadgill, D.S.; Mankowski, L.S.; Cameron , C.E.; Fuller, F.J.; Payne, S.L.
Characterization and mutational studies of equine infectious anemia virus dUTPase
Biochim. Biophys. Acta
1339
181-191
1997
equine infectious anemia virus
Wagaman, P.C.; Hasselkus-Light, C.S.; Henson, M.; Lerner, D.L.; Phillips, T.R.; Elder, J.H.
Molecular cloning and characterization of deoxyuridine triphosphatase from feline immunodeficiency virus (FIV)
Virology
196
451-457
1993
feline immunodeficiency virus
Prangishvili, D.; Klenk, H.P.; Jacobs, G.; Schmiechen, A.; Hanselmann, C.; Holz, I.; Zillig, W.
Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRV
J. Biol. Chem.
273
6024-6029
1998
Sulfolobus islandicus rod-shaped virus 1 (O71028), Sulfolobus islandicus rod-shaped virus 1
Bjoernberg, P.; Nyman, P.O.
The dUTPases from herpes simplex virus type 1 and mpouse mammary tumour virus are less specific than the Escherichia coli enzyme
J. Gen. Virol.
77
3107-3111
1996
Human alphaherpesvirus 1, mouse mammary tumor virus
Pardo, E.G.; Gutierrez, C
Cell cycle-and differentiation stage-dependent variation of dUTPase activity in higher plant cells
Exp. Cell Res.
186
90-98
1990
Allium cepa
Bergman, A.C.; Nyman, P.O.; Larsson, G.
Kinetic properties and stereospecificity of the monomeric dUTPase from herpes simplex virus type 1
FEBS Lett.
441
327-330
1998
Human alphaherpesvirus 1
Huffman, J.L.; Li, H.; White, R.H.; Tainer, J.A.
Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii
J. Mol. Biol.
331
885-896
2003
Methanocaldococcus jannaschii (Q57872), Methanocaldococcus jannaschii
Han, B.W.; Lee, J.Y.; Yang, J.K.; Lee, B.I.; Suh, S.W.
Crystallization and preliminary X-ray crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Saccharomyces cerevisiae
Acta Crystallogr. Sect. D
57
1147-1149
2001
Saccharomyces cerevisiae
Persson, R.; Harkiolaki, M.; McGeehan, J.; Wilson, K.S.
Crystallization and preliminary crystallographic analysis of deoxyuridine 5'-triphosphate nucleotidohydrolase from Bacillus subtilis
Acta Crystallogr. Sect. D
57
876-878
2001
Bacillus subtilis
Bernier-Villamor, V.; Camacho, A.; Hidalgo-Zarco, F.; Perez, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
Characterization of deoxyuridine 5'-triphosphate nucleotidohydrolase from Trypanosoma cruzi
FEBS Lett.
526
147-150
2002
Trypanosoma cruzi
Takacs, E.; Grolmusz, V.K.; Vertessy, B.G.
A tradeoff between protein stability and conformational mobility in homotrimeric dUTPases
FEBS Lett.
566
48-54
2004
Drosophila melanogaster, Escherichia coli
Kovari, J.; Barabas, O.; Takacs, E.; Bekesi, A.; Dubrovay, Z.; Pongracz, V.; Zagyva, I.; Imre, T.; Szabo, P.; Vertessy, B.G.
Altered active site flexibility and a structural metal-binding site in eukaryotic dUTPase: kinetic characterization, folding, and crystallographic studies of the homotrimeric Drosophila enzyme
J. Biol. Chem.
279
17932-17944
2004
Drosophila melanogaster
Barabas, O.; Pongracz, V.; Kovari, J.; Wilmanns, M.; Vertessy, B.G.
Structural insights into the catalytic mechanism of phosphate ester hydrolysis by dUTPase
J. Biol. Chem.
279
42907-42915
2004
Escherichia coli (P06968)
Chan, S.; Segelke, B.; Lekin, T.; Krupka, H.; Cho, U.S.; Kim, M.Y.; So, M.; Kim, C.Y.; Naranjo, C.M.; Rogers, Y.C.; Park, M.S.; Waldo, G.S.; Pashkov, I.; Cascio, D.; Perry, J.L.; Sawaya, M.R.
Crystal structure of the Mycobacterium tuberculosis dUTPase: insights into the catalytic mechanism
J. Mol. Biol.
341
503-517
2004
Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WNS5)
Moroz, O.V.; Harkiolaki, M.; Galperin, M.Y.; Vagin, A.A.; Gonzalez-Pacanowska, D.; Wilson, K.S.
The crystal structure of a complex of Campylobacter jejuni dUTPase with substrate analogue sheds light on the mechanism and suggests the "basic module" for dimeric d(C/U)TPases
J. Mol. Biol.
342
1583-1597
2004
Campylobacter jejuni
Mustafi, D.; Bekesi, A.; Vertessy, B.G.; Makinen, M.W.
Catalytic and structural role of the metal ion in dUTP pyrophosphatase
Proc. Natl. Acad. Sci. USA
100
5670-5675
2003
Escherichia coli
Hidalgo-Zarco, F.; Camacho, A.G.; Bernier-Villamor, V.; Nord, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Leishmania major
Protein Sci.
10
1426-1433
2001
Leishmania major
Harkiolaki, M.; Dodson, E.J.; Bernier-Villamor, V.; Turkenburg, J.P.; Gonzalez-Pacanowska, D.; Wilson, K.S.
The crystal structure of Trypanosoma cruzi dUTPase reveals a novel dUTP/dUDP binding fold
Structure
12
41-53
2004
Trypanosoma cruzi
Barabas, O.; Nemeth, V.; Vertessy, B.G.
Crystallization and preliminary X-ray studies of dUTPase from Mason-Pfizer monkey retrovirus
Acta Crystallogr. Sect. F
F62
399-401
2006
Mason-Pfizer monkey virus
Studebaker, A.W.; Lafuse, W.P.; Kloesel, R.; Williams, M.V.
Modulation of human dUTPase using small interfering RNA
Biochem. Biophys. Res. Commun.
327
306-310
2005
Homo sapiens (P33316), Homo sapiens
Dominguez-Perez, I.; Tellez-Sanz, R.; Leal, I.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
Calorimetric determination of thermodynamic parameters of 2-dUMP binding to Leishmania major dUTPase
Biochim. Biophys. Acta
1702
33-40
2004
Leishmania major
Bekesi, A.; Zagyva, I.; Hunyadi-Gulyas, E.; Pongracz, V.; Kovari, J.; Nagy, A.O.; Erdei, A.; Medzihradszky, K.F.; Vertessy, B.G.
Developmental regulation of dUTPase in Drosophila melanogaster
J. Biol. Chem.
279
22362-22370
2004
Drosophila melanogaster
Nguyen, C.; Kasinathan, G.; Leal-Cortijo, I.; Musso-Buendia, A.; Kaiser, M.; Brun, R.; Ruiz-Perez, L.M.; Johansson, N.G.; Gonzalez-Pacanowska, D.; Gilbert, I.H.
Deoxyuridine triphosphate nucleotidohydrolase as a potential antiparasitic drug target
J. Med. Chem.
48
5942-5954
2005
Homo sapiens, Leishmania donovani, Leishmania major, Plasmodium falciparum
Nguyen, C.; Ruda, G.F.; Schipani, A.; Kasinathan, G.; Leal, I.; Musso-Buendia, A.; Kaiser, M.; Brun, R.; Ruiz-Perez, L.M.; Sahlberg, B.L.; Johansson, N.G.; Gonzalez-Pacanowska, D.; Gilbert, I.H.
Acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase
J. Med. Chem.
49
4183-4195
2006
Homo sapiens, Plasmodium falciparum
Davison, A.J.; Stow, N.D.
New genes from old: redeployment of dUTPase by herpesviruses
J. Virol.
79
12880-12892
2005
Herpesviridae sp.
Zhang, Y.; Moriyama, H.; Homma, K.; Van Etten, J.L.
Chlorella virus-encoded deoxyuridine triphosphatases exhibit different temperature optima
J. Virol.
79
9945-9953
2005
Paramecium bursaria Chlorella virus 1
Galperin, M.Y.; Moroz, O.V.; Wilson, K.S.; Murzin, A.G.
House cleaning, a part of good housekeeping
Mol. Microbiol.
59
5-19
2006
Saccharomyces cerevisiae, Campylobacter jejuni, Escherichia coli, Leishmania major, Staphylococcus aureus, Trypanosoma cruzi, Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis H37Rv (P9WNS5)
Kovari, J.; Imre, T.; Szabo, P.; Vertessy, B.G.
Mechanistic studies of dUTPases
Nucleosides Nucleotides Nucleic Acids
23
1475-1479
2004
Drosophila melanogaster, Escherichia coli
Romeike, B.F.; Boeckeler, A.; Kremmer, E.; Sommer, P.; Krick, C.; Graesser, F.
Immunohistochemical detection of dUTPase in intracranial tumors
Pathol. Res. Pract.
201
727-732
2005
Homo sapiens
Persson, R.; McGeehan, J.; Wilson, K.S.
Cloning, expression, purification, and characterisation of the dUTPase encoded by the integrated Bacillus subtilis temperate bacteriophage SPbeta
Protein Expr. Purif.
42
92-99
2005
Spbetavirus SPbeta
Wu, M.; Shen, J.; Zhan, J.; Yu, Y.
dUTP pyrophosphatase, its appearance in extracellular compartment may serve as a potential biomarker for N-methyl-N-nitro-N-nitrosoguanidine exposure in mammalian cells
Proteomics
6
3001-3007
2006
Homo sapiens
Stout, C.D.
Induced fit, drug design, and dUTPase
Structure
12
2-3
2004
Trypanosoma cruzi
Whittingham, J.L.; Leal, I.; Nguyen, C.; Kasinathan, G.; Bell, E.; Jones, A.F.; Berry, C.; Benito, A.; Turkenburg, J.P.; Dodson, E.J.; Perez, L.M.; Wilkinson, A.J.; Johansson, N.G.; Brun, R.; Gilbert, I.H.; Pacanowska, D.G.; Wilson, K.S.
dUTPase as a platform for antimalarial drug design: structural basis for the selectivity of a class of nucleoside inhibitors
Structure
13
329-338
2005
Plasmodium falciparum
Tarbouriech, N.; Buisson, M.; Seigneurin, J.; Cusack, S.; Burmeister, W.P.
The monomeric dUTPase from Epstein-Barr virus mimics trimeric dUTPases
Structure
14
623
2006
Human gammaherpesvirus 4
-
Liu, X.; Yang, F.
Identification and function of a shrimp white spot syndrome virus (WSSV) gene that encodes a dUTPase
Virus Res.
110
21-30
2005
Shrimp white spot syndrome virus
Zhao, Z.; Ke, F.; Gui, J.; Zhang, Q.
Characterization of an early gene encoding for dUTPase in Rana grylio virus
Virus Res.
123
128-137
2007
Rana grylio iridovirus
Bajaj, M.; Moriyama, H.
Purification, crystallization and preliminary crystallographic analysis of deoxyuridine triphosphate nucleotidohydrolase from Arabidopsis thaliana
Acta Crystallogr. Sect. F
63
409-411
2007
Arabidopsis thaliana (Q9STG6), Arabidopsis thaliana
Lankat-Buttgereit, B.; Lenschen, B.; Schmidt, H.; Goeke, R.
The action of Pdcd4 may be cell type specific: evidence that reduction of dUTPase levels might contribute to its tumor suppressor activity in Bon-1 cells
Apoptosis
13
157-164
2008
Homo sapiens
Varga, B.; Barabas, O.; Takacs, E.; Nagy, N.; Nagy, P.; Vertessy, B.G.
Active site of mycobacterial dUTPase: structural characteristics and a built-in sensor
Biochem. Biophys. Res. Commun.
373
8-13
2008
Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WNS5)
Kajimoto, S.; Horie, M.; Manabe, H.; Masuda, Y.; Shibayama-Imazu, T.; Nakajo, S.; Gong, X.F.; Obama, T.; Itabe, H.; Nakaya, K.
A tyrosine kinase inhibitor, beta-hydroxyisovalerylshikonin, induced apoptosis in human lung cancer DMS114 cells through reduction of dUTP nucleotidohydrolase activity
Biochim. Biophys. Acta
1782
41-50
2008
Homo sapiens (P33316), Homo sapiens
Jiang, Y.L.; Chung, S.; Krosky, D.J.; Stivers, J.T.
Synthesis and high-throughput evaluation of triskelion uracil libraries for inhibition of human dUTPase and UNG2
Bioorg. Med. Chem.
14
5666-5672
2006
Homo sapiens
McCarthy, O.K.; Schipani, A.; Buendia, A.M.; Ruiz-Perez, L.M.; Kaiser, M.; Brun, R.; Pacanowska, D.G.; Gilbert, I.H.
Design, synthesis and evaluation of novel uracil amino acid conjugates for the inhibition of Trypanosoma cruzi dUTPase
Bioorg. Med. Chem. Lett.
16
3809-3812
2006
Trypanosoma cruzi
Varga, B.; Barabas, O.; Kovari, J.; Toth, J.; Hunyadi-Gulyas, E.; Klement, E.; Medzihradszky, K.F.; Toelgyesi, F.; Fidy, J.; Vertessy, B.G.
Active site closure facilitates juxtaposition of reactant atoms for initiation of catalysis by human dUTPase
FEBS Lett.
581
4783-4788
2007
Homo sapiens (P33316), Homo sapiens
Toth, J.; Varga, B.; Kovacs, M.; Malnasi-Csizmadia, A.; Vertessy, B.G.
Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme
J. Biol. Chem.
282
33572-33582
2007
Homo sapiens (P33316), Homo sapiens
Helt, S.S.; Thymark, M.; Harris, P.; Aagaard, C.; Dietrich, J.; Larsen, S.; Willemoes, M.
Mechanism of dTTP inhibition of the bifunctional dCTP deaminase:dUTPase encoded by Mycobacterium tuberculosis
J. Mol. Biol.
376
554-569
2008
Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis H37Rv (P9WNS5)
Cho, Y.; Lee, H.S.; Kim, Y.J.; Kang, S.G.; Kim, S.J.; Lee, J.H.
Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification
Mar. Biotechnol.
9
450-458
2007
Thermococcus onnurineus (A7J1X3)
Guillet, M.; Van Der Kemp, P.A.; Boiteux, S.
dUTPase activity is critical to maintain genetic stability in Saccharomyces cerevisiae
Nucleic Acids Res.
34
2056-2066
2006
Saccharomyces cerevisiae (P33317), Saccharomyces cerevisiae
Kovari, J.; Barabas, O.; Varga, B.; Bekesi, A.; Toelgyesi, F.; Fidy, J.; Nagy, J.; Vertessy, B.G.
Methylene substitution at the alpha-beta bridging position within the phosphate chain of dUDP profoundly perturbs ligand accommodation into the dUTPase active site
Proteins
71
308-319
2008
Escherichia coli (P06968), Escherichia coli
Vertessy, B.G.; Toth, J.
Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases
Acc. Chem. Res.
42
97-106
2009
Homo sapiens (P33316), Homo sapiens
Li, G.L.; Wang, J.; Li, L.F.; Su, X.D.
Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria
Acta Crystallogr. Sect. F
65
339-342
2009
Bacillus subtilis, Streptococcus mutans
Muha, V.; Zagyva, I.; Venkei, Z.; Szabad, J.; Vertessy, B.G.
Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage
Biochem. Biophys. Res. Commun.
381
271-275
2009
Drosophila melanogaster
Palmen, L.G.; Becker, K.; Buelow, L.; Kvassman, J.O.
A double role for a strictly conserved serine: further insights into the dUTPase catalytic mechanism
Biochemistry
47
7863-7874
2008
Escherichia coli (P06968), Escherichia coli
Quesada-Soriano, I.; Leal, I.; Casas-Solvas, J.M.; Vargas-Berenguel, A.; Baron, C.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
Kinetic and thermodynamic characterization of dUTP hydrolysis by Plasmodium falciparum dUTPase
Biochim. Biophys. Acta
1784
1347-1355
2008
Plasmodium falciparum
McCarthy, O.; Musso-Buendia, A.; Kaiser, M.; Brun, R.; Ruiz-Perez, L.M.; Johansson, N.G.; Pacanowska, D.G.; Gilbert, I.H.
Design, synthesis and evaluation of novel uracil acetamide derivatives as potential inhibitors of Plasmodium falciparum dUTP nucleotidohydrolase
Eur. J. Med. Chem.
44
678-688
2009
Plasmodium falciparum
Takacs, E.; Barabas, O.; Petoukhov, M.V.; Svergun, D.I.; Vertessy, B.G.
Molecular shape and prominent role of beta-strand swapping in organization of dUTPase oligomers
FEBS Lett.
583
865-871
2009
Escherichia coli (P06968), Escherichia coli, Homo sapiens (P33316), Homo sapiens, Drosophila melanogaster (Q9V3I1)
Castillo-Acosta, V.M.; Estevez, A.M.; Vidal, A.E.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
Depletion of dimeric all-alpha dUTPase induces DNA strand breaks and impairs cell cycle progression in Trypanosoma brucei
Int. J. Biochem. Cell Biol.
40
2901-2913
2008
Trypanosoma brucei, Trypanosoma brucei 427
Kawahara, A.; Akagi, Y.; Hattori, S.; Mizobe, T.; Shirouzu, K.; Ono, M.; Yanagawa, T.; Kuwano, M.; Kage, M.
Higher expression of deoxyuridine triphosphatase (dUTPase) may predict the metastasis potential of colorectal cancer
J. Clin. Pathol.
62
364-369
2009
Homo sapiens
Musso-Buendia, J.A.; Vidal, A.E.; Kasinthan, G.; Nguyen, C.; Carrero-Lerida, J.; Ruiz-Perez, L.M.; Wilson, K.; Johansson, N.G.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni
J. Enzyme Inhib. Med. Chem.
24
111-116
2009
Campylobacter jejuni, Homo sapiens
Ariza, M.E.; Glaser, R.; Kaumaya, P.T.; Jones, C.; Williams, M.V.
The EBV-encoded dUTPase activates NF-kappa B through the TLR2 and MyD88-dependent signaling pathway
J. Immunol.
182
851-859
2009
Human gammaherpesvirus 4
Homma, K.; Moriyama, H.
Crystallization and crystal-packing studies of Chlorella virus deoxyuridine triphosphatase
Acta Crystallogr. Sect. F
65
1030-1034
2009
Chlorella virus, Chlorella virus IL-3A
Siggaard, J.H.; Johansson, E.; Vognsen, T.; Helt, S.S.; Harris, P.; Larsen, S.; Willemoes, M.
Concerted bifunctionality of the dCTP deaminase-dUTPase from Methanocaldococcus jannaschii: a structural and pre-steady state kinetic analysis
Arch. Biochem. Biophys.
490
42-49
2009
Methanocaldococcus jannaschii
Quesada-Soriano, I.; Casas-Solvas, J.M.; Recio, E.; Ruiz-Perez, L.M.; Vargas-Berenguel, A.; Gonzalez-Pacanowska, D.; Garcia-Fuentes, L.
Kinetic properties and specificity of trimeric Plasmodium falciparum and human dUTPases
Biochimie
92
178-186
2010
Homo sapiens, Plasmodium falciparum
Merenyi, G.; Konya, E.; Vertessy, B.G.
Drosophila proteins involved in metabolism of uracil-DNA possess different types of nuclear localization signals
FEBS J.
277
2142-2156
2010
Drosophila melanogaster (Q9V3I1)
Takacs, E.; Nagy, G.; Leveles, I.; Harmat, V.; Lopata, A.; Toth, J.; Vertessy, B.G.
Direct contacts between conserved motifs of different subunits provide major contribution to active site organization in human and mycobacterial dUTPases
FEBS Lett.
584
3047-3054
2010
Homo sapiens, Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis H37Rv (P9WNS5)
Freeman, L.; Buisson, M.; Tarbouriech, N.; Van der Heyden, A.; Labbe, P.; Burmeister, W.P.
The flexible motif V of Epstein-Barr virus deoxyuridine 5'-triphosphate pyrophosphatase is essential for catalysis
J. Biol. Chem.
284
25280-25289
2009
Human gammaherpesvirus 4 (P03195), Human gammaherpesvirus 4
Palmen, L.G.; Kvassman, J.O.
Differential inhibition of homotrimeric dUTPases by the 3-azido derivative of dideoxy-UTP
J. Enzyme Inhib. Med. Chem.
25
146-150
2010
Escherichia coli, equine infectious anemia virus, Homo sapiens
Pecsi, I.; Leveles, I.; Harmat, V.; Vertessy, B.G.; Toth, J.
Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase
Nucleic Acids Res.
38
7179-7186
2010
Homo sapiens, Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis H37Rv (P9WNS5)
Reina, R.; Bertolotti, L.; Dei Giudici, S.; Puggioni, G.; Ponti, N.; Profiti, M.; Patta, C.; Rosati, S.
Small ruminant lentivirus genotype E is widespread in Sarda goat
Vet. Microbiol.
144
24-31
2010
no activity in caprine lentivirus genotype E
Tchigvintsev, A.; Singer, A.U.; Flick, R.; Petit, P.; Brown, G.; Evdokimova, E.; Savchenko, A.; Yakunin, A.F.
Structure and activity of the Saccharomyces cerevisiae dUTP pyrophosphatase DUT1, an essential housekeeping enzyme
Biochem. J.
437
243-253
2011
Saccharomyces cerevisiae (P33317), Saccharomyces cerevisiae
Baragana, B.; McCarthy, O.; Sanchez, P.; Bosch-Navarrete, C.; Kaiser, M.; Brun, R.; Whittingham, J.L.; Roberts, S.M.; Zhou, X.X.; Wilson, K.S.; Johansson, N.G.; Gonzalez-Pacanowska, D.; Gilbert, I.H.
beta-Branched acyclic nucleoside analogues as inhibitors of Plasmodium falciparum dUTPase
Bioorg. Med. Chem.
19
2378-2391
2011
Plasmodium falciparum (Q8II92), Plasmodium falciparum
Recio, E.; Musso-Buendia, A.; Vidal, A.E.; Ruda, G.F.; Kasinathan, G.; Nguyen, C.; Ruiz-Perez, L.M.; Gilbert, I.H.; Gonzalez-Pacanowska, D.
Site-directed mutagenesis provides insights into the selective binding of trityl derivatives to Plasmodium falciparum dUTPase
Eur. J. Med. Chem.
46
3309-3314
2011
Plasmodium falciparum (Q8II92), Plasmodium falciparum
Ramalho, T.C.; Caetano, M.S.; Josa, D.; Luz, G.P.; Freitas, E.A.; da Cunha, E.F.
Molecular modeling of Mycobacterium tuberculosis dUTpase: docking and catalytic mechanism studies
J. Biomol. Struct. Dyn.
28
907-917
2011
Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WNS5)
Miyakoshi, H.; Miyahara, S.; Yokogawa, T.; Chong, K.T.; Taguchi, J.; Endoh, K.; Yano, W.; Wakasa, T.; Ueno, H.; Takao, Y.; Nomura, M.; Shuto, S.; Nagasawa, H.; Fukuoka, M.
Synthesis and discovery of N-carbonylpyrrolidine- or N-sulfonylpyrrolidine-containing uracil derivatives as potent human deoxyuridine triphosphatase inhibitors
J. Med. Chem.
55
2960-2969
2012
Homo sapiens (P33316), Homo sapiens
Miyahara, S.; Miyakoshi, H.; Yokogawa, T.; Chong, K.T.; Taguchi, J.; Muto, T.; Endoh, K.; Yano, W.; Wakasa, T.; Ueno, H.; Takao, Y.; Fujioka, A.; Hashimoto, A.; Itou, K.; Yamamura, K.; Nomura, M.; Nagasawa, H.; Shuto, S.; Fukuoka, M.
Discovery of highly potent human deoxyuridine triphosphatase inhibitors based on the conformation restriction strategy
J. Med. Chem.
55
5483-5496
2012
Homo sapiens (P33316), Homo sapiens
Pecsi, I.; Hirmondo, R.; Brown, A.C.; Lopata, A.; Parish, T.; Vertessy, B.G.; Toth, J.
The dUTPase enzyme is essential in Mycobacterium smegmatis
PLoS ONE
7
e37461
2012
Mycolicibacterium smegmatis (A0QW08), Mycolicibacterium smegmatis, Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 (A0QW08), Mycobacterium tuberculosis H37Rv (P9WNS5)
Dabrowski, S.; Kiaer Ahring, B.
Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR
Protein Expr. Purif.
31
72-78
2003
Pyrococcus woesei (Q6T180), Pyrococcus woesei, Pyrococcus woesei DSM 3773 (Q6T180)
Garca-Nafra, J.; Timm, J.; Harrison, C.; Turkenburg, J.P.; Wilson, K.S.
Tying down the arm in Bacillus dUTPase: structure and mechanism
Acta Crystallogr. Sect. D
69
1367-1380
2013
Bacillus subtilis (O31801), Bacillus subtilis (O34919), Bacillus subtilis
Leveles, I.; Nmeth, V.; Szab, J.E.; Harmat, V.; Nyri, K.; Bendes, ..; Papp-Kdr, V.; Zagyva, I.; Rna, G.; Ozohanics, O.; Vkey, K.; Tth, J., Vrtessy, B.G.
Structure and enzymatic mechanism of a moonlighting dUTPase
Acta Crystallogr. Sect. D
69
2298-2308
2013
Dubowvirus dv11 (Q8SDV3)
Hemsworth, G.R.; Gonzalez-Pacanowska, D.; Wilson, K.S.
On the catalytic mechanism of dimeric dUTPases
Biochem. J.
456
81-88
2013
Trypanosoma brucei (Q57ZH3), Trypanosoma brucei 927/4 (Q57ZH3)
Hampton, S.E.; Schipani, A.; Bosch-Navarrete, C.; Recio, E.; Kaiser, M.; Kahnberg, P.; Gonzalez-Pacanowska, D.; Johansson, N.G.; Gilbert, I.H.
Investigation of acyclic uridine amide and 5-amido nucleoside analogues as potential inhibitors of the Plasmodium falciparum dUTPase
Bioorg. Med. Chem.
21
5876-5885
2013
Plasmodium falciparum
Zhu, G.; Wei, Y.; Zhang, Q.; Ji, X.; Lin, L.
Expression and enzyme activity of Sulfolobus virus dUTPase gene
Chin. J. Appl. Environ. Biol.
20
712-716
2014
Sulfolobus virus STSV2 (K9MDK5)
-
Horvath, A.; Batki, J.; Henn, L.; Lukacsovich, T.; Rona, G.; Erdelyi, M.; Vertessy, B.G.
dUTPase expression correlates with cell division potential in Drosophila melanogaster
FEBS J.
282
1998-2013
2015
Drosophila melanogaster (Q9V3I1), Drosophila melanogaster
Ariza, M.; Glaser, R.; Williams, M.
Human herpesviruses-1 encoded dUTPases: A family of proteins that modulate dendritic cell function and innate immunity
Front. Microbiol.
5
504
2014
Human alphaherpesvirus 2, Human alphaherpesvirus 3, Human betaherpesvirus 6A (P30007), Human gammaherpesvirus 8 (P88942), Human gammaherpesvirus 8, Human gammaherpesvirus 8 type M (P88942), Human betaherpesvirus 6A GS (P30007)
Tormo-Mas, M.A.; Donderis, J.; Garcia-Caballer, M.; Alt, A.; Mir-Sanchis, I.; Marina, A.; Penades, J.R.
Phage dUTPases control transfer of virulence genes by a proto-oncogenic G protein-like mechanism
Mol. Cell
49
947-958
2013
Dubowvirus dv80alpha (A4ZF98)
Barabas, O.; Nemeth, V.; Bodor, A.; Perczel, A.; Rosta, E.; Kele, Z.; Zagyva, I.; Szabadka, Z.; Grolmusz, V.I.; Wilmanns, M.; Vertessy, B.G.
Catalytic mechanism of ?-phosphate attack in dUTPase is revealed by X-ray crystallographic snapshots of distinct intermediates, 31P-NMR spectroscopy and reaction path modelling
Nucleic Acids Res.
41
10542-10555
2013
Mason-Pfizer monkey virus (O92810)
Szabo, J.E.; Nemeth, V.; Papp-Kadar, V.; Nyiri, K.; Leveles, I.; Bendes, A.A.; Zagyva, I.; Rona, G.; Palinkas, H.L.; Besztercei, B.; Ozohanics, O.; Vekey, K.; Liliom, K.; Toth, J.; Vertessy, B.G.
Highly potent dUTPase inhibition by a bacterial repressor protein reveals a novel mechanism for gene expression control
Nucleic Acids Res.
42
11912-11920
2014
Staphylococcus phage phi11 (Q8SDV3)
Guiomar, P.; Andras, H.; Beata, G.; Luis, M.; Dolores, G.; Requena, C.; Vidal, A.
The nucleotidohydrolases DCTPP1 and dUTPase are involved in the cellular response to decitabine
Biochem. J.
473
2635-2643
2016
Homo sapiens, Homo sapiens (P33316)
Benedek, A.; Temesvary-Kis, F.; Khatanbaatar, T.; Leveles, I.; Suranyi, E.V.; Szabo, J.E.; Wunderlich, L.; Vertessy, B.G.
The role of a key amino acid position in species-specific proteinaceous dUTPase inhibition
Biomolecules
9
221
2019
Escherichia coli (P06968), Escherichia coli
Alam, M.S.; Moriyama, H.; Matsumoto, M.
Enzyme kinetics of dUTPase from the planarian Dugesia ryukyuensis
BMC Res. Notes
12
163
2019
Dugesia ryukyuensis
Inoguchi, N.; Chaiseeda, K.; Yamanishi, M.; Kim, M.K.; Jang, Y.; Bajaj, M.; Chia, C.P.; Becker, D.F.; Moriyama, H.
Structural insights into the mechanism defining substrate affinity in Arabidopsis thaliana dUTPase the role of tryptophan 93 in ligand orientation
BMC Res. Notes
8
784
2015
Arabidopsis thaliana (Q9STG6), Arabidopsis thaliana
Kumar, H.; Kehrer, J.; Singer, M.; Reinig, M.; Santos, J.M.; Mair, G.R.; Frischknecht, F.
Functional genetic evaluation of DNA house-cleaning enzymes in the malaria parasite dUTPase and Ap4AH are essential in Plasmodium berghei but ITPase and NDH are dispensable
Expert Opin. Ther. Targets
23
251-261
2019
Plasmodium berghei (A0A509AI86), Plasmodium berghei, Plasmodium berghei ANKA (A0A509AI86)
Benedek, A.; Horvath, A.; Hirmondo, R.; Ozohanics, O.; Bekesi, A.; Modos, K.; Revesz, A.; Vekey, K.; Nagy, G.N.; Vertessy, B.G.
Potential steps in the evolution of a fused trimeric all-beta dUTPase involve a catalytically competent fused dimeric intermediate
FEBS J.
283
3268-3286
2016
Drosophila virilis (B4LTG5), Drosophila virilis
Benedek, A.; Poeloeskei, I.; Ozohanics, O.; Vekey, K.; Vertessy, B.G.
The Stl repressor from Staphylococcus aureus is an efficient inhibitor of the eukaryotic fruitfly dUTPase
FEBS Open Bio
8
158-167
2018
Drosophila melanogaster
Kerepesi, C.; Szabo, J.E.; Papp-Kadar, V.; Dobay, O.; Szabo, D.; Grolmusz, V.; Vertessy, B.G.
Life without dUTPase
Front. Microbiol.
7
1768
2016
Aeromonas hydrophila, Campylobacter jejuni, Escherichia coli, no activity in Staphylococcus aureus strain RN450, Aeromonas hydrophila ATCC 7966, Escherichia coli ATCC 25922
Nagy, G.N.; Suardiaz, R.; Lopata, A.; Ozohanics, O.; Vekey, K.; Brooks, B.R.; Leveles, I.; Toth, J.; Vertessy, B.G.; Rosta, E.
Structural characterization of arginine fingers identification of an arginine finger for the pyrophosphatase dUTPases
J. Am. Chem. Soc.
138
15035-15045
2016
Mycobacterium tuberculosis (P9WNS5), Mycobacterium tuberculosis H37Rv (P9WNS5)
Zang, K.; Li, F.; Ma, Q.
The dUTPase of white spot syndrome virus assembles its active sites in a noncanonical manner
J. Biol. Chem.
293
1088-1099
2018
Escherichia coli, white spot syndrome virus (Q77J78), white spot syndrome virus
Li, G.; Wang, C.; Yang, M.; Cao, L.; Fu, D.; Liu, X.; Sun, D.; Chen, C.; Wang, Y.; Jia, Z.; Yang, C.; Guo, Y.; Rao, Z.
Structural insight into the African swine fever virus dUTPase reveals a novel folding pattern in the dUTPase family
J. Virol.
94
e01698-19
2019
African swine fever virus (A0A2X0SE53), African swine fever virus
Li, C.; Chai, Y.; Song, H.; Weng, C.; Qi, J.; Sun, Y.; Gao, G.F.
Crystal structure of African swine fever virus dUTPase reveals a potential drug target
mBio
10
e02483-19
2019
African swine fever virus (A0A2X0SE53), African swine fever virus
Rotoli, S.M.; Jones, J.L.; Caradonna, S.J.
Cysteine residues contribute to the dimerization and enzymatic activity of human nuclear dUTP nucleotidohydrolase (nDut)
Protein Sci.
27
1797-1809
2018
Homo sapiens (P33316), Homo sapiens
Hizi, A.; Herzig, E.
dUTPase the frequently overlooked enzyme encoded by many retroviruses
Retrovirology
12
70
2015
bovine immunodeficiency virus, Caenorhabditis elegans, Caprine arthritis encephalitis virus, equine infectious anemia virus, Escherichia coli (P06968), feline immunodeficiency virus, Homo sapiens (P33316), Homo sapiens, Human endogenous retrovirus K, Human gammaherpesvirus 4, Human gammaherpesvirus 8, Jembrana disease virus, Mason-Pfizer monkey virus, mouse mammary tumor virus, Murid gammaherpesvirus 4, Mycobacterium tuberculosis, Plasmodium falciparum, Saccharomyces cerevisiae, Visna-maedi virus
Nyiri, K.; Mertens, H.D.T.; Tihanyi, B.; Nagy, G.N.; Kohegyi, B.; Matejka, J.; Harris, M.J.; Szabo, J.E.; Papp-Kadar, V.; Nemeth-Pongracz, V.; Ozohanics, O.; Vekey, K.; Svergun, D.I.; Borysik, A.J.; Vertessy, B.G.
Structural model of human dUTPase in complex with a novel proteinaceous inhibitor
Sci. Rep.
8
4326
2018
Homo sapiens
Deng, L.; Griffin, B.D.; Pei, Y.; Leishman, D.; McBey, B.A.; Sharif, S.; Nagy, E.
Fowl aviadenovirus 9 dUTPase plays a role in regulation of the host immune response
Viral Immunol.
30
662-670
2017
Fowl adenovirus 9
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