Crystallization (Comment) | Organism |
---|---|
mutant enzymes H145W and H145A in complex with alpha,beta-imido-dUTP, hanging drop vapor diffusion method, using 50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 1.20-1.75 M ammonium sulfate and 10% (v/v) glycerol in a 1:1 ratio | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
F158A | the mutant shows severely decreased catalytic activity compared to the wild type enzyme | Homo sapiens |
F158W | the mutant shows decreased catalytic activity compared to the wild type enzyme | Homo sapiens |
H145A | the mutant shows decreased catalytic activity compared to the wild type enzyme | Mycobacterium tuberculosis |
H145W | the mutant shows decreased catalytic activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,beta-imido-dUTP | - |
Homo sapiens | |
alpha,beta-imido-dUTP | - |
Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
dUTP | wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
0.0036 | - |
dUTP | mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
0.0052 | - |
dUTP | mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mycobacterium tuberculosis | P9WNS5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WNS5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dUTP + H2O | - |
Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mycobacterium tuberculosis | dUMP + diphosphate | - |
? | |
dUTP + H2O | - |
Mycobacterium tuberculosis H37Rv | dUMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DUT | - |
Homo sapiens |
DUT | - |
Mycobacterium tuberculosis |
dUTPase | - |
Homo sapiens |
dUTPase | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
dUTP | mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
5.8 | - |
dUTP | wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
6.8 | - |
dUTP | mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62 | - |
dUTP | mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
1900 | - |
dUTP | mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens | |
5800 | - |
dUTP | wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C | Homo sapiens |