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Literature summary for 3.6.1.23 extracted from

  • Pecsi, I.; Leveles, I.; Harmat, V.; Vertessy, B.G.; Toth, J.
    Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase (2010), Nucleic Acids Res., 38, 7179-7186.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzymes H145W and H145A in complex with alpha,beta-imido-dUTP, hanging drop vapor diffusion method, using 50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 1.20-1.75 M ammonium sulfate and 10% (v/v) glycerol in a 1:1 ratio Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
F158A the mutant shows severely decreased catalytic activity compared to the wild type enzyme Homo sapiens
F158W the mutant shows decreased catalytic activity compared to the wild type enzyme Homo sapiens
H145A the mutant shows decreased catalytic activity compared to the wild type enzyme Mycobacterium tuberculosis
H145W the mutant shows decreased catalytic activity compared to the wild type enzyme Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
alpha,beta-imido-dUTP
-
Homo sapiens
alpha,beta-imido-dUTP
-
Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
0.0036
-
dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
0.0052
-
dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mycobacterium tuberculosis P9WNS5
-
-
Mycobacterium tuberculosis H37Rv P9WNS5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dUTP + H2O
-
Homo sapiens dUMP + diphosphate
-
?
dUTP + H2O
-
Mycobacterium tuberculosis dUMP + diphosphate
-
?
dUTP + H2O
-
Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
?

Synonyms

Synonyms Comment Organism
DUT
-
Homo sapiens
DUT
-
Mycobacterium tuberculosis
dUTPase
-
Homo sapiens
dUTPase
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.32
-
dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
5.8
-
dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
6.8
-
dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
62
-
dUTP mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
1900
-
dUTP mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens
5800
-
dUTP wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C Homo sapiens