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3.6.1.23: dUTP diphosphatase

This is an abbreviated version!
For detailed information about dUTP diphosphatase, go to the full flat file.

Word Map on EC 3.6.1.23

Reaction

dUTP
+
H2O
=
dUMP
 +
diphosphate

Synonyms

DCD-DUT, dCTP deaminase-dUTPase, dCTP deaminase:dUTPase, deoxyuridine 5'-triphosphate, deoxyuridine 5'-triphosphate nucleotide hydrolase, deoxyuridine 5'-triphosphate nucleotidohydrolase, deoxyuridine 5'-triphosphate pyrophosphatase, deoxyuridine 50-triphosphate nucleotidohydrolase, deoxyuridine 5’-triphosphate nucleotidohydrolase, deoxyuridine nucleotidohydrolase, deoxyuridine triphosphatase, deoxyuridine triphosphate nucleotidohydrolase, deoxyuridine triphosphate pyrophosphatase, deoxyuridine-5'-triphosphate nucleotidohydrolase, deoxyuridine-triphosphate pyrophosphatase, desoxyuridine 5'-triphosphatase, desoxyuridine 5'-triphosphate nucleotidohydrolase, desoxyuridine-triphosphatase, DURP, DUT, DUT-N, DUT1, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPae, dUTPase, dUTPase-dUDPase, dUTPase-related protein, E165R, More, ORF 54, ORF8, P18, PIP4, Pr95, STSV2_30, Tb927.7.5160, U45, YncF, YosS

ECTree

     3 Hydrolases
         3.6 Acting on acid anhydrides
             3.6.1 In phosphorus-containing anhydrides
                3.6.1.23 dUTP diphosphatase

Cloned

Cloned on EC 3.6.1.23 - dUTP diphosphatase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the dut gene in the runaway-replication plasmid pKN402A and in the lambdapL-promoter plasmid pHUB2
-
DNA and amino acid sequence determination and analysis, His-tagged enzyme expression in Escherichia coli
DNA and amino acid sequence determination and analysis, the gene locates on chromosome 11, overexpression in Escherichia coli strain BL21(DE3)
-
DURP gene of herpesvirinae, localization in the genome and organization, overview
-
enzyme expression
enzyme expression in Escherichia coli strain BL21(DE3)
expressed in different Escherichia coli strains
-
expressed in Escherichia coli
expressed in Escherichia coli (DE3) Rosetta cells
expressed in Escherichia coli BL21
expressed in Escherichia coli BL21 (DE3) Rosetta cells
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) (pLysS) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) Rosetta cells
-
expressed in Escherichia coli BL21(DE3)-pLysS cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21-Gold (DE3) as His-tag fusion protein
expressed in Escherichia coli BL21-Gold(DE3) cells
expressed in Escherichia coli JM103 (DE3) cells
expressed in Escherichia coli strain BW286
-
expressed in HeLa cells, in KB cells, or in RAJI cells
-
expression as C-terminal domain fusion protein with the nucleocapsid protein in Escherichia coli
-
expression in Escherichia coli
expression in Escherichia coli under the control of a bacteriophage T7 promoter
-
expression in Schneider 2 cells
-
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
expression of N-terminally His-tagged wild-type dUTPase and mutant F158W
expression of the core enzyme lacking the 28-residue-long C-terminal fly-specific segment
expression of the His-tagged nuclear isoform and its F158W mutant
expression of the recombinant enzyme
-
gene BLLF3, overexpression of His-tagged enzyme in Escherichia coli
-
gene dut, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene DUT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, temporal expression pattern during the viral infection course, overexpression as EGFP-fusion enzyme in the cytoplasm of EPC cells, no effects on virus replication by enzyme overexpression, overview
-
gene DUT, recombinant expression
-
gene DUT1, DNA and amino acid sequence determination and analysis of DUT1 alleles, genotyping, expression of wild-type and mutant enzymes in yeast strains
gene wdut, DNA and amino acid sequence determination and analysis, expression in Escherichia coli
-
gene yosS encoded by the Bacillus subtilis temperate bacteriophage SPbeta, DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli strain BL21(DE3)
Spbetavirus SPbeta
-
genes yncF and yosS, DNA and amino acid sequence determination and analysis, high level overexpression of N-terminally His6-tagged enzymes in Escherichia coli strain BL21(DE3)
-
isolation of cDNA clones and characterization
-
native and mutant enzyme
overexpression in Escherichia coli
-
overexpression in Escherichia coli strain BL21
-
overexpression in Escherichia coli strain BL21(DE3)
-
recombinant expression
the expression of N-terminal His(6)-tagged Pwo dUTPase is performed in Escherichia coli BL21(DE3)pLysS and Eschwerichia coli Rosetta(DE3)pLysS strain that contains plasmid encoding additional copies of rare Escherichia coli tRNAs. Escherichia coli Rosetta(pLysS) strain is found with two times higher expression yield of His(6)-tagged Pwo dUTPase than Escherichia coli BL21(DE3)pLysS. The His(6)-tagged Pwo dUTPase is purified on Ni(2+)-IDA-Sepharose, dialyzed, and the enzyme activity is investigated. THe His(6)-tag domain has no influence on dUTP hydrolytic activity. dUTP is generated during PCR from dCTP, which inhibits the polymerization of DNA catalyzed by DNA polymerase with 3'-5' exonuclease activity
two isozymes, DNA and amino acid sequence determination and analysis, developmental expression analysis, overview
-
two naturally occuring variants of the enzyme are expressed as recombinant proteins in Escherichia coli
-