3.6.1.23: dUTP diphosphatase
This is an abbreviated version!
For detailed information about dUTP diphosphatase, go to the full flat file.
Word Map on EC 3.6.1.23
-
3.6.1.23
-
uracil
-
dump
-
trimer
-
thymidylate
-
misincorporation
-
glycosylase
-
herpes
-
uracil-dna
-
simplex
-
drug development
-
homotrimeric
-
herpesviruses
-
integrase
-
fluoropyrimidines
-
uracil-containing
-
lentiviruses
-
pyrophosphatases
-
sapis
-
ebv-encoded
-
herv-k
-
2'-deoxyuridine
-
varicella-zoster
-
fiv
-
diagnostics
-
medicine
-
pharmacology
-
synthesis
- 3.6.1.23
- uracil
- dump
- trimer
- thymidylate
-
misincorporation
- glycosylase
-
herpes
-
uracil-dna
- simplex
- drug development
-
homotrimeric
- herpesviruses
-
integrase
-
fluoropyrimidines
-
uracil-containing
- lentiviruses
- pyrophosphatases
-
sapis
-
ebv-encoded
- herv-k
- 2'-deoxyuridine
-
varicella-zoster
- fiv
- diagnostics
- medicine
- pharmacology
- synthesis
Reaction
Synonyms
DCD-DUT, dCTP deaminase-dUTPase, dCTP deaminase:dUTPase, deoxyuridine 5'-triphosphate, deoxyuridine 5'-triphosphate nucleotide hydrolase, deoxyuridine 5'-triphosphate nucleotidohydrolase, deoxyuridine 5'-triphosphate pyrophosphatase, deoxyuridine 50-triphosphate nucleotidohydrolase, deoxyuridine 5’-triphosphate nucleotidohydrolase, deoxyuridine nucleotidohydrolase, deoxyuridine triphosphatase, deoxyuridine triphosphate nucleotidohydrolase, deoxyuridine triphosphate pyrophosphatase, deoxyuridine-5'-triphosphate nucleotidohydrolase, deoxyuridine-triphosphate pyrophosphatase, desoxyuridine 5'-triphosphatase, desoxyuridine 5'-triphosphate nucleotidohydrolase, desoxyuridine-triphosphatase, DURP, DUT, DUT-N, DUT1, dUTP nucleotidohydrolase, dUTP pyrophosphatase, dUTPae, dUTPase, dUTPase-dUDPase, dUTPase-related protein, E165R, More, ORF 54, ORF8, P18, PIP4, Pr95, STSV2_30, Tb927.7.5160, U45, YncF, YosS
ECTree
3.6.1.23 dUTP diphosphataseAdvanced search results
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results (Substrates Products
Substrates Products on EC 3.6.1.23 - dUTP diphosphatase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2',3'-dideoxyUTP + H2O
2',3'-dideoxy-UMP + diphosphate
-
76% of the activity with dUTP
-
-
?
alpha,beta-imido-dUTP + H2O
dUMP + amino-diphosphate
O92810
-
-
-
?
dITP + H2O
dIMP + diphosphate
shows also significant activity against dITP
-
-
?
2'-deoxy-5-fluoro-UTP + H2O
2'-deoxy-5-fluoro-UMP + diphosphate
-
most specific substrate
-
-
?
2'-deoxy-5-fluoro-UTP + H2O
2'-deoxy-5-fluoro-UMP + diphosphate
-
most specific substrate
-
-
?
dCTP + H2O
dCMP + diphosphate
about 10fold lower activity with dCTP compared to dUTP
-
-
?
dUDP + H2O
deoxyuridine + diphosphate
-
the enzyme is highly specific for deoxyuridine nucleotides
-
-
?
dUDP + H2O
deoxyuridine + diphosphate
-
the enzyme is highly specific for deoxyuridine nucleotides
-
-
?
dUTP + H2O
?
-
the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation
-
-
?
dUTP + H2O
?
-
the enzyme is important in preventing dUTP from being incorporated into DNA and may have a significant role in both the synthesis of thymidine- and diphosphate-dependent phosphorylation
-
-
?
dUTP + H2O
?
-
the enzyme prevents a deleterious incorporation of uracil into DNA
-
-
?
dUTP + H2O
?
-
lethality of a mutation in the dut gen, deoxyuridine triphosphatase. The dut gene product might have an essential function apart from its deoxyuridine triphosphatase activity
-
-
?
dUTP + H2O
?
-
the enzyme may generally perform an essential role in DNA replication and therefore can serve as a target enzyme for the development of chemotherapeutic compounds
-
-
?
dUTP + H2O
?
-
enzyme suppresses incorporation of uracil into DNA and provides a pool of dUMP, the precursor of dTTP
-
-
?
dUTP + H2O
?
-
elimination of dUTP, by dUTPase, is vital since its misincorporation into DNA by DNA polymerases can initiate a damaging iterative repair and misincorporation cycle, resulting in DNA fragmentation and cell death, the anti-tumour activity of folate agonists and thymidylate synthase inhibitors is thought to rely on UTP misincorporation. dUTPase activity is an ideal point of intervention in both chemotherapy and anti-retroviral therapy
-
-
?
dUTP + H2O
?
-
enzyme is involved in controlling intracellular dUTP levels
-
-
?
dUTP + H2O
?
-
enzyme suppresses incorporation of uracil into DNA and provides a pool of dUMP, the precursor of dTTP
-
-
?
dUTP + H2O
dUMP + diphosphate
the enzyme is responsible for maintaining a low dUTP level in order to avoid the incorporation of uracil into DNA, and for providing dUMP as a substrate for deoxythymidine triphosphate biosynthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for deoxyuridine nucleotides
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides substrate for dTTP synthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme prevents incorporation of uracil into DNA by strict regulation of the cellular dUTP to dTTP ratio, developmental regulation of the isozymes at mRNA and protein levels
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
an efficient preventive repair pathway, avoiding uracil in DNA, is generally present that keeps cellular dUTP/dTTP levels at a much decreased value to prevent use of dUTP as a building block by DNA polymerases. The enzyme responsible for this sanitizing role is dUTPase, a ubiquitous protein
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the essential enzyme is responsible for preventive DNA repair via exclusion of uracil, overview, lack of negative enzyme regulation leads to thymine-less cell death in cells performing active DNA synthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
a preventive DNA repair enzyme, contributes to maintain the appropriate cellular dUTP/dTTP ratio by catalyzing dUTP hydrolysis, dUTPase is essential for viability
-
-
?
dUTP + H2O
dUMP + diphosphate
substrate binding structure, the strictly conserved residue Ser72 is involved in phosphate chain binding, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
biological roles and functions of dUTPase-related proteins and domains, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
-
?
dUTP + H2O
dUMP + diphosphate
dUTPase is essential for DNA integrity and is an important survival factor for cancer cells
-
-
?
dUTP + H2O
dUMP + diphosphate
dUTPase is essential in controlling relative cellular levels of dTTP/dUTP, both of which can be incorporated into DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
enzyme inhibition is involved in apoptosis in cancer cells and decreases the sensitivity of cells for 5-fluorouracil, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is one of the primary enzymes that prevent the incorporation and accumulation of deoxyuridine in genomic DNA, and is essential for viability
-
-
?
dUTP + H2O
dUMP + diphosphate
substrate synthesis, overview, the enzymatic cycle is limited by a single rate-limiting step that occurs before or is concomitant with proton release
-
-
?
dUTP + H2O
dUMP + diphosphate
dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase enzyme family eliminate excess dUTP as one moethod to avoid uracil nucleotides incorporation into DNA, besides exchanging dUTP for dTTP as a second method, overview
dUMP is required for de novo biosynthesis of thymine
-
?
dUTP + H2O
dUMP + diphosphate
-
dUTPase catalyzes the hydrolysis of dUTP to dUMP and diphosphate controlling the incorporation of uracil into DNA genomes
-
-
?
dUTP + H2O
dUMP + diphosphate
catalysis proceeds by way of an SN2 mechanism, a water molecule initiates in-line nucleophilic attack, conformational changes during the reaction take place in the C-terminus. The dUTPase binding pocket is highly specific for uracil, structural basis, overview. Phosphate chain coordination involves Mg2+ and is analogous to that of DNA polymerases. Catalytically competent and noncompetent conformation of the bound substrate, nodelling, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for deoxyuridine nucleotides
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is essential in the lytic cycle of the virus
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, essential for replication and cell survival
-
-
?
dUTP + H2O
dUMP + diphosphate
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
?
dUTP + H2O
dUMP + diphosphate
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
dUTPase is essential to eliminate dUTP for DNA integrity and provide dUMP for thymidylate biosynthesis
-
-
?
dUTP + H2O
dUMP + diphosphate
development of a robust and continuous dUTPase enzyme activity assay method using phenolred indicator, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
?
dUTP + H2O
dUMP + diphosphate
-
dUMP is required for biosynthesis of dTTP, overview
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
-
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme has two major roles in maintaining the correct free nucleotide balance in the cell. It provides dUMP, a major cellular source for dTMP which is in turn needed for dTTP formation. It also maintains the concentration of dUTP lower than that of dTTP thereby minimizing mistaken incorporation of dUTP into DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
dUTPase catalyzes the hydrolysis of the alpha,beta-diphosphate bond of dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme regulates the levels of dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the enzyme is highly specific for dUTP
-
-
?
dUTP + H2O
dUMP + diphosphate
the enzyme plays an essential role in nucleotide biosynthesis, dUMP is required for de novo synthesis of dTTP. The enzyme maintains low cellular ratios of dUTP:dTTP, thus preventing the misincorporation of uracil into chromosomal DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
dUTPase has a dual role in pyrimidine nucleotide metabolism, it provides the substrate for the thymidylate synthase allowing the formation of dTTP. Secondly, the enzyme is responsible for keeping dUTP intracellular levels low, avoiding its misincorporation into nascent DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
the reaction produces the primary source of substrate for synthesis of dTTP and reduces the intracellular dUTP concentration, thus limiting the misincorporation of uracil into DNA
-
-
?
dUTP + H2O
dUMP + diphosphate
-
substrate binding structure analysis, overview
-
-
?
UTP + H2O
UMP + diphosphate
substrate for mutant enzyme Y88A
-
-
?
additional information
?
-
the enzyme does not hydrolyze dATP, dTTP, dCTP, or dGTP
-
-
-
additional information
?
-
-
the enzyme does not hydrolyze dATP, dTTP, dCTP, or dGTP
-
-
-
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
-
comparison of the Drosophila melanogaster and the Escherichia coli enzymes
-
-
?
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
comparison of the Drosophila melanogaster and the Escherichia coli enzymes
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
-
overexpression of dUTPase is associated with resistance to chemotherapeutic agents targeting thymidilate biosynthesis
-
-
?
additional information
?
-
-
the enzyme limits the activation or degradation of 5-fluorouracil, overview. The expression of dUTPase is significantly different between primary tumours and their corresponding metastatic tumour
-
-
?
additional information
?
-
-
the enzyme has immunomodulatory function in pathogenesis, treatment of human monocyte-derived macrophages with dUTPase from Ebstein-Barr virus induces the expression of proinflammatory cytokines, e.g. interleukin-6, through activation of NF-kappaB via TLR2 receptor, but not TLR3 or TLR4, requiring the adaptor molecule MyD88, but not CD14, overview
-
-
?
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
-
the enzyme binds non-cooperatively to 2'-dUMP
-
-
?
additional information
?
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
the bifunctional dCTP deaminase:dUTPase shows very similar affinities for dCTP and deoxyuridine triphosphate as substrates, overview
-
-
?
additional information
?
-
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
-
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
the bifunctional dCTP deaminase:dUTPase shows very similar affinities for dCTP and deoxyuridine triphosphate as substrates, overview
-
-
?
additional information
?
-
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
-
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
the enzyme cannot hydrolyze alpha-beta-imido-dUTP
-
-
?
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
alpha,beta-imido dUTP is a non-hydrolysable substrate
-
-
?
additional information
?
-
-
alpha,beta-imido dUTP is a non-hydrolysable substrate
-
-
?
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
additional information
?
-
the enzyme is essential for survival
-
-
?
additional information
?
-
-
procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation
-
-
?
additional information
?
-
-
procyclic cells with reduced dUTPase activity exhibit an altered cell cycle and DNA fragmentation
-
-
?
additional information
?
-
-
biological functions in housekeeping, overview
-
-
?
additional information
?
-
-
substrate specificity, no hydrolysis of canonical NTPs, overview
-
-
?
