Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTMP | - |
Homo sapiens | |
dTMP | - |
Plasmodium falciparum | |
fluorodeoxy-UMP | - |
Homo sapiens | |
fluorodeoxy-UMP | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.00173 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.00187 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.0061 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.29 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.438 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.465 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.523 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.54 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.571 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.687 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.761 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
1.13 | - |
dATP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
1.96 | - |
dGTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-5-fluoro-UTP + H2O | most specific substrate | Homo sapiens | 2'-deoxy-5-fluoro-UMP + diphosphate | - |
? | |
2'-deoxy-5-fluoro-UTP + H2O | most specific substrate | Plasmodium falciparum | 2'-deoxy-5-fluoro-UMP + diphosphate | - |
? | |
beta-L-2'-dUTP + H2O | - |
Homo sapiens | beta-L-2'-dUMP + diphosphate | - |
? | |
beta-L-2'-dUTP + H2O | - |
Plasmodium falciparum | beta-L-2'-dUMP + diphosphate | - |
? | |
dATP + H2O | - |
Homo sapiens | dAMP + diphosphate | - |
? | |
dATP + H2O | - |
Plasmodium falciparum | dAMP + diphosphate | - |
? | |
dCTP + H2O | - |
Homo sapiens | dCMP + diphosphate | - |
? | |
dCTP + H2O | - |
Plasmodium falciparum | dCMP + diphosphate | - |
? | |
dGTP + H2O | - |
Homo sapiens | dGMP + diphosphate | - |
? | |
dGTP + H2O | - |
Plasmodium falciparum | dGMP + diphosphate | - |
? | |
dTTP + H2O | - |
Homo sapiens | dTMP + diphosphate | - |
? | |
dTTP + H2O | - |
Plasmodium falciparum | dTMP + diphosphate | - |
? | |
dUTP + H2O | most specific substrate | Homo sapiens | dUMP + diphosphate | - |
? | |
dUTP + H2O | most specific substrate | Plasmodium falciparum | dUMP + diphosphate | - |
? | |
UTP + H2O | - |
Homo sapiens | UMP + diphosphate | - |
? | |
UTP + H2O | - |
Plasmodium falciparum | UMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Homo sapiens |
deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Plasmodium falciparum |
dUTPase | - |
Homo sapiens |
dUTPase | - |
Plasmodium falciparum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
dGTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.00265 | - |
dATP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.00525 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.0061 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.0092 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.01121 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.01278 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.01971 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
0.02172 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
0.1616 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
110 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
600 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
6600 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
7100 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum |