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1.3
dITP
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
4.1
dUTP-Co2+
-
pH 7.0, 25°C, recombinant enzyme
13.3
dUTP-Mg2+
-
pH 7.0, 25°C, recombinant enzyme
5.3
dUTP-Mn2+
-
pH 7.0, 25°C, recombinant enzyme
5.3
UTP
mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
1.5
dATP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.7
dCTP
-
pH 7.5, 25°C
1.6
dCTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
2.9
dGTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.1
dTTP
-
pH 7.5, 25°C
1.4
dTTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
0.0003
dUTP
D90N mutant enzyme, pH 7.8, room temperature
0.00089
dUTP
mutant enzyme G143STOP, at pH 7.5 and 20°C
0.0035
dUTP
mutant enzyme T138STOP, at pH 7.5 and 20°C
0.0045
dUTP
mutant enzyme R140K/H145W, at pH 7.5 and 20°C
0.007
dUTP
-
mutant enzyme D49N/F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.024
dUTP
mutant enzyme D28N/H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.04
dUTP
mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.04
dUTP
mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.053
dUTP
mutant enzyme R161K, at pH 7.5 and 20°C
0.06
dUTP
mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.18
dUTP
mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
0.32
dUTP
-
mutant enzyme F158A, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
0.45
dUTP
mutant enzyme R24T, at pH 7.5 and 20°C
0.51
dUTP
mutant enzyme R24T/S115R, at pH 7.5 and 20°C
0.53
dUTP
mutant enzyme D170L, at pH 7.5 and 20°C
0.67
dUTP
mutant enzyme DELTA134-153, at pH 7.5 and 20°C
0.7
dUTP
mutant enzyme H145W, at pH 7.5 and 20°C
0.88
dUTP
DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C
0.92
dUTP
mutant enzyme D34N, at pH 7.5 and 20°C
1.2
dUTP
mutant enzyme H145W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
1.2
dUTP
wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
1.2
dUTP
mutant enzyme F166Y, at pH 7.5 and 20°C
1.22
dUTP
wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C
1.3
dUTP
mutant enzyme Q114A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
1.4
dUTP
wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
1.6
dUTP
wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
1.7
dUTP
mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C
1.7
dUTP
mutant enzyme DELTA170-171, at pH 7.5 and 20°C
2.6
dUTP
mutant enzyme R158E, at pH 7.5 and 20°C
2.6
dUTP
mutant enzyme S115R, at pH 7.5 and 20°C
2.7
dUTP
-
mutant enzyme R166S, at pH 7.5 and 20°C
3
dUTP
wild type enzyme, at pH 7.5 and 20°C
3.1
dUTP
mutant enzyme R158W, at pH 7.5 and 20°C
3.1
dUTP
mutant enzyme R24T/S115R/D170G, at pH 7.5 and 20°C
3.2
dUTP
mutant enzyme R158A, at pH 7.5 and 20°C
3.3
dUTP
-
pH 7.6, 25°C, recombinant enzyme, in presence of 1 mM MgCl2
3.3
dUTP
mutant enzyme R111A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
3.3
dUTP
-
mutant enzyme T22R/R116S, at pH 7.5 and 20°C
3.4
dUTP
-
pH 7.0, 25°C, recombinant enzyme
3.7 - 5.8
dUTP
pH 7.5, 25°C, recombinant wild-type and mutant enzymes
4.5
dUTP
-
pH 7.5, 25°C, in the absence of added metal ions
4.7
dUTP
at pH 7.5 and 20°C
5.5
dUTP
mutant enzyme K150E, at pH 7.5 and 20°C
5.8
dUTP
-
wild type enzyme, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
5.9
dUTP
mutant enzyme K150A, at pH 7.5 and 20°C
6.2
dUTP
mutant enzyme D170G, at pH 7.5 and 20°C
6.3
dUTP
-
mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
6.58
dUTP
pH 7.4, 20°C, recombinant wild-type enzyme
6.8
dUTP
-
pH 8.5, 50°C, recombinant enzyme
6.8
dUTP
-
mutant enzyme F158W, in 1 mM HEPES pH 7.5 containing 100 mM KCl and 5 mM MgCl2, at 20°C
7.4
dUTP
mutant enzyme Y88A, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
7.4
dUTP
wild type enzyme, at pH 7.5 and 20°C
7.4
dUTP
mutant enzyme V160D, at pH 7.5 and 20°C
8
dUTP
recombinant wild-type and mutant enzymes
9.3
dUTP
-
wild type enzyme, at pH 7.5 and 20°C
9.6
dUTP
wild type enzyme, in 50 mM HEPES (pH 7.5), 5 mM MgCl2, 1 mM MnCl2, 0.5 mM NiCl2, at 30°C
9.7
dUTP
-
pH 7.4, 37°C, recombinant enzyme
11
dUTP
wild-type enzyme, pH 7.8, room temperature
11.33
dUTP
mutant enzyme F46A, at pH 8.0 and 25°C
11.67
dUTP
mutant enzyme K96A, at pH 8.0 and 25°C
11.67
dUTP
wild type enzyme, at pH 8.0 and 25°C
12
dUTP
mutant enzyme I117A, at pH 8.0 and 25°C
12.3
dUTP
-
pH 8.0, 25°C, recombinant enzyme
14.7
dUTP
-
pH 7.5, 25°C, in the presence of Mg2+ or Mn2+
18
dUTP
Spbetavirus SPbeta
-
pH 7.5, 25°C, recombinant enzyme
24.2
dUTP
-
pH 7.5, 25°C, in the presence of bis(acetylacetonato)oxovanadium(IV)
27.3
dUTP
-
pH 7.5, 25°C, in the presence of VO2+ or bis(acetylacetonato)oxovanadium(IV)
34
dUTP
-
in the presence of 5 mM Mg2+, at pH 8.2 and 25°C
48
dUTP
-
in the presence of 5 mM Mn2+, at pH 8.2 and 25°C