Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

Cloned

Cloned on EC 3.4.24.27 - thermolysin

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an efficient method of production of enzymatically active thermolysin by refolding the protein expressed as inclusion bodies in Escherichia coli. Association of high pressure and alkaline pH is very efficient for solubilization
cloned in Bacillus subtilis DB117, plasmids subcloned in Escherichia coli
-
expressed in Escherichia coli strain K12 JM109
-
expression of wild-type and mutant enzymes in Bacillus subtilis by vector pTB51, the enzymes are secreted to the culture medium
-
expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109
expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109 and secretion to the cell culture medium
-
expression of wild-type and mutant enzymes in Escherichia coli strain T5KC991
-
gene HSPA, DNA and amino acid sequence determination and analysis, sequence comparison
gene npr, DNA and amino acid sequence determination, expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109
-
gene npr, expression of wild-type and mutant enzymes in Escherichia coli K12 strain JM109
-
genes npr and nprT, DNA and amino acid sequence determination and analysis, expression in Escherichia coli and Bacillus subtilis
-
genes npr and nprT, DNA and amino acid sequence determination and analysis, expression in Escherichia coli and Bacillus subtilis, different methods, expression of tagged mature enzyme, proenzyme, or mutant enzyme in inclusion bodies or in the cytosol or medium as soluble protein, overview
-
recombinant overexpression of wild-type and mutant enzymes in Escherichia coli