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3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

Purification

Purification on EC 3.4.24.27 - thermolysin

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by affinity chromatography
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by Gly-D-Phe affinity chromatography, coupling to the resin by epichlorohydrin, 1,4-butandiol diglycidyl ether, or 1,6-hexanediol diglycidyl ether, method optimization and evaluation, overview
-
commercial enzyme powder further purified to remove salts
commercial preparation, used without further purification
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evaluation of Gly-D-Phe, Gly-L-Leu, and D-Phe as affinity ligands for thermolysin, each of the ligands is immobilized to a resin. The optimum pH for adsorption of thermolysin is pH 5.0 to pH 6.0 for each of the ligands, affinity chromatography method development, adsorption isotherms, overview
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expression as single polypeptide pre-proenzyme in Escherichia coli, secretion into medium as mature enzyme
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incubation of impure enzyme at 37°C causes proteolysis of impurities, whereas the protease remains active and uncleaved
-
mobile phase effects in the high-performance affinity purification
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native enzyme to homogeneity from the culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, gel filtration
recombinant enzyme, different methods, e.g. by Gly-D-Phe or bacitracin affinity, ion exchange, and hydrophobic interaction chromatography, gel filtration and ammonium sulfate fractionation, detailed overview
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recombinant extracellular wild-type and mutant enzymes from Bacillus subtilis culture medium by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration
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recombinant mature wild-type and mutant enzymes 7.2-11fold from Escherichia coli by hydrophobic interaction and affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli K12 strain JM109 by hydrophobic interaction and Gly-D-Phe affinity chromatography
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recombinant wild-type and mutant enzymes from Escherichia coli K12 strain JM109 to homogeneity by Gly-D-Phe heat treatment at 60°C for 20 min, affinity chromatography and gel filtration
recombinant wild-type and mutated thermolysin
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wild-type and mutated thermolysin
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