Cloned (Comment) | Organism |
---|---|
gene HSPA, DNA and amino acid sequence determination and analysis, sequence comparison | Halobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 85% inhibition at 1 mM, 93% at 5 mM | Halobacillus sp. | |
Cu2+ | 39% inhibition at 1 mM, 52% at 5 mM | Halobacillus sp. | |
EDTA | 77% inhibition at 1 mM, 86% at 5 mM | Halobacillus sp. | |
EGTA | 59% inhibition at 1 mM, 98% at 5 mM | Halobacillus sp. | |
additional information | no inhibition by PMSF | Halobacillus sp. | |
Ni2+ | 39% inhibition at 1 mM, 87% at 5 mM | Halobacillus sp. | |
Zn2+ | zinc metalloprotease, no effect on activity by exogenous Zn2+ at 1 mM, inhibitory by 69% at 5 mM | Halobacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Halobacillus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates 8% at 1 mM, 15% at 5 mM | Halobacillus sp. | |
Ca2+ | activates 36% at 1 mM, 48% at 5 mM | Halobacillus sp. | |
Mg2+ | activates 12% at 1 mM, 37% at 5 mM | Halobacillus sp. | |
Mn2+ | activates 12% at 1 mM, 25% at 5 mM | Halobacillus sp. | |
Zn2+ | zinc metalloprotease, no efect on activity by exogenous Zn2+ at 1 mM, inhibitory at 5 mM | Halobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Halobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacillus sp. | K0EFJ2 | isolated from a marine sediment of South China Sea, gene HSPA | - |
Halobacillus sp. SCSIO 20089 | K0EFJ2 | isolated from a marine sediment of South China Sea, gene HSPA | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity from the culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, gel filtration | Halobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
373 | - |
purified native enzyme, pH 8.0, 30°C, substrate feather | Halobacillus sp. |
489 | - |
purified native enzyme, pH 8.0, 30°C, substrate elastin | Halobacillus sp. |
697 | - |
purified native enzyme, pH 8.0, 30°C, substrate hemoglobin | Halobacillus sp. |
855 | - |
purified native enzyme, pH 8.0, 30°C, substrate collagen | Halobacillus sp. |
1468 | - |
purified native enzyme, pH 8.0, 30°C, substrate bovine serum albumin | Halobacillus sp. |
2899 | - |
purified native enzyme, pH 8.0, 30°C, substrate gelatin | Halobacillus sp. |
3077 | - |
purified native enzyme, pH 8.0, 30°C, substrate casein | Halobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bovine serum albumin + H2O | - |
Halobacillus sp. | ? | - |
? | |
Bovine serum albumin + H2O | - |
Halobacillus sp. SCSIO 20089 | ? | - |
? | |
casein + H2O | - |
Halobacillus sp. | L-tyrosine + ? | - |
? | |
Collagen + H2O | - |
Halobacillus sp. | ? | - |
? | |
Collagen + H2O | - |
Halobacillus sp. SCSIO 20089 | ? | - |
? | |
Elastin + H2O | - |
Halobacillus sp. | ? | - |
? | |
feather + H2O | - |
Halobacillus sp. | ? | - |
? | |
Gelatin + H2O | - |
Halobacillus sp. | ? | - |
? | |
Gelatin + H2O | - |
Halobacillus sp. SCSIO 20089 | ? | - |
? | |
Hemoglobin + H2O | - |
Halobacillus sp. | ? | - |
? | |
Hemoglobin + H2O | - |
Halobacillus sp. SCSIO 20089 | ? | - |
? | |
additional information | the enzyme has low affinity for alkaline or acid P1' residues, poor activity with N-[3-(2-furyl)acryloyl]-Glu-Glu-OH , no activity with N-[3-(2-furyl)acryloyl]-Ala-Arg-OH | Halobacillus sp. | ? | - |
? | |
additional information | the enzyme has low affinity for alkaline or acid P1' residues, poor activity with N-[3-(2-furyl)acryloyl]-Glu-Glu-OH , no activity with N-[3-(2-furyl)acryloyl]-Ala-Arg-OH | Halobacillus sp. SCSIO 20089 | ? | - |
? | |
N-[3-(2-furyl)acryloyl]-Gly-Phe-NH2 + H2O | - |
Halobacillus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Halobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
hspA | - |
Halobacillus sp. |
thermolysin-like protease | - |
Halobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Halobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
cold-adapted thermolysin-like protease, 20% activity remains | Halobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Halobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | active at broad pH intervals | Halobacillus sp. |