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3.4.24.27: thermolysin

This is an abbreviated version!
For detailed information about thermolysin, go to the full flat file.

Word Map on EC 3.4.24.27

Reaction

preferential cleavage: -/-Leu > -/-Phe =

Synonyms

Bacillus thermoproteolyticus neutral proteinase, EC 3.4.24.4, hspA, LIC13322, Neutral metalloproteinase, NprM, protease type X, proteinase type X, Proteinase, Bacillus thermoproteolyticus neutral, protex 14L, Thermoase, thermoase PC10F, Thermoase Y10, thermolysin, thermolysin-like protease, Thermostable neutral proteinase, TL, TLN, TLP, TLP-ste

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.27 thermolysin

KM Value

KM Value on EC 3.4.24.27 - thermolysin

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
(7-methoxycoumarin-4yl) acetyl-L-Pro-L-Leu-Gly-L-Leu-[N3-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH2
-
pH and temperature not specified in the publication
0.00449
(europium(III) complex of a modified terpyridine)-K1K2K2GFSAK2K-black hole quencher 2
-
pH and temperature not specified in the publication
-
0.0533
5,6-carboxyfluorescein-PAGLAC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
0.0024
5,6-carboxyfluorescein-PAGLAC(Cy5)-NH2
-
pH 7.4, 30°C
0.0776
5,6-carboxyfluorescein-PSVAGLAGGC(Alexa Fluor 647)-NH2
-
pH 7.4, 30°C
-
0.0176
5,6-carboxyfluorescein-PSVAGLAGGC(Cy5)-NH2
-
pH 7.4, 30°C
0.0116
5,6-carboxyfluorescein-PVAGLAGC(Cy5)-NH2
-
pH 7.4, 30°C
0.65 - 0.72
Benzyloxycarbonyl-(4-nitro)Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
2.8
Benzyloxycarbonyl-Asp
-
pH 5.0 or pH 6.0
0.033 - 0.076
Benzyloxycarbonyl-Asp-Phe methyl ester
0.98 - 1.39
Benzyloxycarbonyl-Gly-Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
0.55 - 1.52
Benzyloxycarbonyl-Phe-Leu-Ala
-
depending on mode of preparation of the form of enzyme
0.2
dansyl-Ala-Ala-Phe-Ala
-
-
2
dansyl-Ala-Leu-Ala
-
-
0.91
dansyl-Ala-Phe-Ala
-
-
13
dansyl-Gly-Gly-Leu-Gly
-
-
5
dansyl-Gly-Leu-Gly
-
-
0.69
dansyl-Gly-Leu-Phe
-
-
0.08 - 0.09
Dansyl-Gly-Phe-Ala
-
depending on assay method
0.77
dansyl-Gly-Phe-Gly
-
-
0.3
dansyl-Gly-Phe-Phe
-
-
0.3 - 0.6
GFA
0.4 - 2.5
GFL
1.3 - 3.5
GFS
0.7 - 12
GFSA
250 - 300
L-Phe methyl ester
5.8 - 12.9
L-phenylalanine methyl ester
0.2 - 1.985
leucine enkephalin
0.00395
N,N'-diBoc-dityrosyl-(Ile-isoniazid)2
-
pH 7.5, 37°C
0.00191
N,N'-diBoc-dityrosyl-(Phe-isoniazid)2
-
pH 7.5, 37°C
20 - 39.2
N-(benzyloxycarbonyl)-L-Phe
12 - 20
N-benzyloxycarbonyl-Gly-L-Leu amide
0.42 - 0.57
N-benzyloxycarbonyl-L-Asp-L-Phe-methyl ester
10 - 20
N-carbobenzoxy-Gly-L-Leu-NH2
0.06 - 1.29
N-carbobenzoxy-L-Asp-L-Phe methyl ester
0.7 - 2.1
N-carbobenzoxy-L-Asp-L-Phe-methyl ester
49 - 105
N-carbobenzoxy-L-aspartic acid
0.38 - 0.49
N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester
0.77
N-[3-(2-furyl)acryloyl]-Gly-L-Leu amide
-
mutant enzyme S53D, in 40 mM HEPES-NaOH buffer at pH 7.5 containing 10 mM CaCl2, at 25°C
0.62
Phe-Leu-Ala-NH(CH2)2NH-dansyl
-
-
0.2
[3H]Tyr-Gly-Gly-Phe-Leu
pH 8.0, 37°C
additional information
additional information
-